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Mixed strain culture for the disposal of food waste, and food waste disposal method using same

A food waste, mixed strain technology, applied in the direction of using bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of energy consumption, can not be used normally to remove the sprinkler system, etc., achieve high degradation activity, improve The effect of degradation rate

Inactive Publication Date: 2012-12-19
AHN GOOK PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, heating is required to reduce the moisture content, which leads to a large amount of energy consumption. In order to maintain a low moisture content, there is a disadvantage that the sprinkler system required for the removal of degradation products cannot be used normally.

Method used

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  • Mixed strain culture for the disposal of food waste, and food waste disposal method using same
  • Mixed strain culture for the disposal of food waste, and food waste disposal method using same
  • Mixed strain culture for the disposal of food waste, and food waste disposal method using same

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0059] Preparation of mixed strains

[0060] The stock solution (stock) of Brevibacillus Potsdam and Bacillus licheniformis was added to the screening medium (nutrient broth) at a concentration of 1%, and cultivated in a 45°C incubator for 24 hours while stirring, and the Trullis wine The stock solution of yeast was added to the screening medium (YM broth) at a concentration of 1%, and cultured in a 37°C incubator for 24 hours while stirring. Each 400 mL of bacterial culture liquid was mixed with 200 mL of yeast culture liquid to prepare 1000 mL of mixed strains.

[0061] The obtained mixed strain was deposited in the Korean Type Culture Collection on November 10, 2009 with the accession number KTCT 11585BP. (Hereafter, the above-mentioned mixed strain is named "nATS-AG".)

experiment example 1

[0062] Experimental example 1: Determination of growth rate according to temperature, initial pH value and salinity

[0063] experimental method

[0064] The growth curve of nATS-AG was determined according to different temperature, initial pH value and salinity. The medium is composed of 5g / L peptone, 10g / L gel, 2.5g / L yeast extract, 5g / L soluble starch, 3g / L malt extract, 3g / L cellulose, 2g / L L of beef extract, 5g / L of NaCl composition. The increase in the number of individuals is expressed as a change in absorbance at a wavelength of 600 nm in a spectrophotometer.

[0065] The growth rate of the mixed strain ATS-1 (hereinafter referred to as "control strain") disclosed in Korean Patent No. 0580857 of Bacillus smithii and thermophilic yeast was measured by the same method as above, and compared with nATS-AG Compare.

experiment example 1-1

[0066] Experimental example 1-1: Growth rate according to temperature

[0067] The growth rates of nATS-AG and control strains at 37°C, 45°C, 60°C and 70°C were measured by the above experimental method, and the results were displayed in Figure 7 with Figure 8 middle.

[0068] Unlike the control strain, nATS-AG can still grow fully 24 hours after the initiation of culture, even at 45°C. Saccharomyces cerevisiae (Kazachstania telluris) grows vigorously at 37°C, showing quite high OD values ​​compared to other temperatures.

[0069] It can be seen from the above results that the growth rate of nATS-AG at high temperature far exceeds that of the control strain.

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Abstract

The present invention relates to a mixed strain culture for the disposal of food waste, and more particularly, to a mixed strain culture for the disposal of food waste which has high degradation activity on cellulose, amylose, protein, and fat at a wide range of temperatures, pH levels, and salinities, and which can degrade food waste having a high moisture content and therefore can degrade food waste in an efficient manner. The present invention also relates to a food waste disposal method using the mixed strain culture.

Description

technical field [0001] The invention relates to a mixed bacterial strain for treating food waste, in particular to a mixed bacterial strain for treating food waste and a method for using the bacterial strain to treat food waste. The mixed bacterial strain has high degrading activity on cellulose, starch, protein and fat in a wide range of temperature, pH and salinity ranges, and can degrade food waste with high water content, thereby effectively degrading food waste. Background technique [0002] South Korea produces 50,346 tons of domestic waste every day (based on 2007). Over the past 20 years, with population growth, urban industrialization, and urban concentration, waste production has increased dramatically. Judging from the status quo of waste disposal, in 1991, 89.2% of domestic waste was landfilled, and only 7.9% was reused. While the landfill disposal rate decreased to 47.0%, the reuse rate increased to 41.3%. [0003] Among them, most of the treatment of food was...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00
CPCC12N1/20C12R1/01C12P39/00C12R1/10C12R2001/10C12N1/205C12R2001/01
Inventor 郑有婷安泰爽郑多运赵安娜李恩莹李明善
Owner AHN GOOK PHARMA CO LTD
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