Composite micro-organism agent based on bacillus subtilis BS1 and application thereof

A technology of Bacillus subtilis and composite bacterial agent, which is applied in the field of composite bacterial agent and application field based on Bacillus subtilis BS1, to achieve the effects of shortening time, increasing composting temperature, and significantly reducing the effect

Active Publication Date: 2019-08-30
HUAZHONG AGRI UNIV
View PDF9 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus is the dominant flora in the compost. The Bacillus subtilis isolated from the compost in the present invention has strong protease, amylase and cellulase activities, is sensitive to various antibiotics, and has a strong effect on ammonium nitrogen. The ability to use it, screen it for the development of composting compound bacteria agent, and obtain good application effect, especially have a certain reduction effect on antibiotic resistance genes and pathogenic bacteria in composting. Currently, there is no report on this aspect of research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite micro-organism agent based on bacillus subtilis BS1 and application thereof
  • Composite micro-organism agent based on bacillus subtilis BS1 and application thereof
  • Composite micro-organism agent based on bacillus subtilis BS1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Obtaining of Bacillus subtilis BS1:

[0041] It is isolated and purified from pig manure compost, and identified as Bacillus subtilis according to its physiological and biochemical indicators and sequence analysis. This strain has amylase, protease and cellulase activities; it is sensitive to many different types of antibiotics, sensitive antibiotics include: tetracycline , chloramphenicol, erythromycin, norfloxacin, kanamycin, co-trimoxazole; and can use a variety of nitrogen sources, including (NH 4 ) 2 SO 4 , CO(NH 2 ) 2 , NH 4 Cl, KNO 3 、NaNO 2 , NH 3 ·H 2 O.

[0042] (1) Colony morphological characteristics:

[0043] Round or irregular shape, rough and opaque surface, dirty white ( figure 1 ).

[0044] (2) Culture characteristics

[0045] Bacillus subtilis can survive in basal medium LB (sodium chloride, tryptone, yeast extract, adjust pH to 7.0 with 5mol / L NaOH, 121°C, sterilize for 20min), and its optimum growth temperature is 30°C- 37°C. Colony gr...

Embodiment 2

[0050] Biological characteristics of Bacillus subtilis BS1:

[0051] Using three methods (disc, single colony spot, punching) to explore the antibacterial test of BS1 against Salmonella, Pasteurella, and Streptococcus in vitro, no antibacterial zone was found, BS1 has a fast propagation speed and large colonies, and it may pass Competing for nutrients and ecological sites and other means to win development space for itself, squeeze the living environment of pathogenic microorganisms, so as to achieve the purpose of reducing pathogenic bacteria. The sensitivity test of the BS1 strain to antibiotics showed that the diameter of the inhibition zone was greater than 15mm, and it was sensitive to multiple antibiotics, indicating that the strain did not carry antibiotic resistance genes and would not increase the horizontal transfer of resistance genes.

[0052] Through enzyme activity test, it was found that BS1 had higher protease activity, amylase activity and cellulase activity. ...

Embodiment 3

[0055] A compound microbial agent, after mixing Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium and Saccharomyces cerevisiae at a volume ratio of 1L:1L:1L:2L, centrifuge and resuspend in 0.5L sterilized LB liquid medium Prepared; Used in the following examples;

[0056] Before mixing, the effective bacterium concentration of described Bacillus subtilis is 8x10 8 CFU / mL, the effective bacterial concentration of Bacillus licheniformis is 1.7x10 8 CFU / mL; effective bacterial concentration of Bacillus megaterium 1.18x10 8 CFU / mL; the effective bacterial concentration of Saccharomyces cerevisiae is 6.2x10 7 CFU / mL;

[0057] The Bacillus subtilis is Bacillus subtilis BS1, and the preservation number is: CCTCC NO: M 2019185; the Bacillus licheniformis is Bacillus licheniformis L3; the Bacillus megaterium is Bacillus megaterium C2; Yeast Y2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The present invention belongs to the fields of microorganisms and applications thereof, and specifically discloses a composite micro-organism agent based on bacillus subtilis BS1 and an application thereof. The bacillus subtilis is preserved in China Center for Type Culture Collection and has a preservation number of CCCCC NO:M 2019185. The composite micro-organism agent prepared from the bacillussubtilis strain and saccharomyces cerevisiae, bacillus megaterium and bacillus licheniformis can be used for manure composting of livestock and poultry. The composite micro-organism agent can increase composting temperature in compost, shortens time to reach a high temperature, conducts deodorization, fixes nitrogen, and has significant reduction effects on various antibiotic resistance genes andpathogenic micro-organism in the compost.

Description

technical field [0001] The invention belongs to the field of microbes and their applications, and in particular relates to a composite bacterial agent based on bacillus subtilis BS1 and its application; the composite bacterial agent can be used for livestock and poultry manure composting. Background technique [0002] With the rapid development of my country's breeding industry, livestock and poultry manure pollution has become one of the sources of agricultural non-point source pollution. The large amount of livestock and poultry manure discharged and the difficulty of treatment not only seriously affected the health and production performance of livestock and poultry, but also caused huge pressure on the ecological environment such as the surrounding water body and soil. At the same time, antibiotic resistance genes (ARGs) carried by livestock and poultry manure, as a new type of pollutant, can spread in the environment and are difficult to control and eliminate. These fa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C05F15/00C05F11/08C05F17/00C05G3/00C12R1/125C12R1/10C12R1/11C12R1/865
CPCC05F3/00C05F11/08C05F17/00C05G3/00C12N1/18C12N1/20Y02W30/40
Inventor 吴健曹蕊李珂莫尚昆任竹青
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap