A kind of bacillus subtilis, composite microbial bacterial agent, organic matrix and its preparation and application

A compound microbial inoculant, Bacillus subtilis technology, applied in the preparation of organic fertilizers, biological organic part treatment, microorganisms and other directions, can solve the problem of insignificant effect of microorganisms in starting composting, shorten the composting time, high water content, The effect of raising the temperature of compost fermentation

Active Publication Date: 2022-03-15
上海成土养木环保科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It aims to solve the problem of insignificant composting effect initiated by microorganisms in the existing high-temperature aerobic composting technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of bacillus subtilis, composite microbial bacterial agent, organic matrix and its preparation and application
  • A kind of bacillus subtilis, composite microbial bacterial agent, organic matrix and its preparation and application
  • A kind of bacillus subtilis, composite microbial bacterial agent, organic matrix and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Obtaining and identification, preservation of embodiment 1 Bacillus subtilis

[0071] The present invention obtains a strain of Bacillus subtilis BS-W1 from livestock and poultry feces high-temperature composting raw materials. The strain has been preserved in the China Center for Type Culture Collection on October 19, 2017, and the preservation number is CCTCC NO: M 2017589.

[0072] The bacillus subtilis isolated in the invention can withstand high temperature composting environment (70°C). And it can secrete a variety of enzymes to efficiently degrade compost materials such as manure and straw.

[0073] After morphological identification and 16S rRNA, ITS-2 and rbcL amplified sequence developmental tree analysis, it was determined that the strain was Bacillus subtilis (according to the international naming rules: genus name + species name + strain name to name the strain, genus name, The species name and strain name are respectively Bacillus, subtilis and BS-W1), na...

Embodiment 2

[0074] Embodiment 2 High-temperature aerobic composting composite microbial bacterial agent

[0075] The Bacillus subtilis BS-W1 of the invention can be used for high-temperature aerobic composting. Generally, it is formulated with other microorganisms into a composite microbial agent for high-temperature aerobic composting.

[0076] The range of parts by weight between the components in the composite microbial agent 1 is: 1 part by weight of Bacillus subtilis, 5 parts by weight of Aspergillus niger, 1 part by weight of Bacillus amyloliquefaciens, 5 parts by weight of Candida tropicalis, 1 part by weight of Trichoderma, 3 parts by weight of Streptomyces grisea, 1 part by weight of Phanerochaete chrysosporium, 3 parts by weight of Pseudomonas fluorescens, 1 part by weight of Lactobacillus brevis, and 3 parts by weight of Bacillus longum. .

[0077] The range of parts by weight between the components in the composite microbial agent 2 is: 5 parts by weight of Bacillus subtilis...

Embodiment 3

[0087] Embodiment 3 Utilizes cattle raising waste and apple pomace to prepare organic matrix

[0088] Organic matrix formula in the present embodiment:

[0089] The parts by weight of each raw material are: 30 parts by weight of waste cattle breeding fermented bedding material, 30 parts by weight of cow solid manure, 20 parts by weight of apple pomace, 8 parts by weight of biochar, 6 parts by weight of rice bran, 3 parts by weight of peat, pearl 3 parts by weight of rock, and 1 part by weight of composite microbial bacterial agent is added to ferment. The composition and parts by weight of the composite microbial bacterial agent in the present embodiment are: 3 parts of Bacillus subtilis, 5 parts of Aspergillus niger, 3 parts of Bacillus amyloliquefaciens, 3 parts of Candida tropicalis, 3 parts of Trichoderma reesei, 1 part of Streptomyces rubrum, 3 parts of Phanerochaete chrysosporium, 1 part of Pseudomonas fluorescens, 1 part of Lactobacillus brevis, and 1 part of Bacillus ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of organic substrates, and in particular relates to a bacillus subtilis, a composite microbial agent, an organic substrate and its preparation and application. The Bacillus subtilis BS‑W1 of the present invention has a preservation number of: CCTCC NO: M 2017589. The bacillus subtilis can be prepared into a composite microbial bacterial agent, which can be composted at high temperature and then prepared into an organic matrix. Since the Bacillus subtilis strain has strong tolerance under 70°C fermentation conditions, and can secrete a variety of hydrolytic enzymes to efficiently degrade macromolecular organic substances such as protein, starch, and cellulose in raw materials, the compost center temperature can Rise to 70°C. Therefore, compared with the conventional composting technology, the composting fermentation temperature is increased, thereby significantly increasing the organic matter and total nutrient content, and can more effectively kill pathogenic bacteria, parasite eggs, weed seeds, etc.

Description

technical field [0001] The invention belongs to the field of organic substrates, and in particular relates to a bacillus subtilis, a composite microbial agent, an organic substrate and its preparation and application. Background technique [0002] Organic substrate cultivation refers to the use of organic matter such as livestock and poultry manure, crop straw, mushroom residue, peat, sawdust, etc., after high-temperature fermentation treatment, mixed in a certain proportion to form a relatively stable and buffering full-nutrient cultivation substrate raw material. The cultivation substrate made entirely of organic matter has poor air permeability and water retention. Therefore, in order to improve the physical and chemical properties of the cultivation substrate, vermiculite, perlite, river sand, cinder and other inorganic substances can be mixed with it in a certain proportion to form an organic-inorganic substrate. Model cultivation substrate. [0003] At present, the so...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05G3/80C05F11/08C05F17/20C05F17/50C12R1/125C12R1/685C12R1/07C12R1/74C12R1/885C12R1/465C12R1/645C12R1/39C12R1/24
CPCC12N1/14C12N1/16C12N1/20C05D9/00C05F11/08C05F17/00C05G3/80C12R2001/125C12N1/205C05F3/00C05F5/002C05F9/04C05F11/02Y02W30/40
Inventor 张久琴张文高潘家庆
Owner 上海成土养木环保科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap