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Bacillus subtilis and application thereof in manure compost deodorization

A Bacillus subtilis, compost deodorization technology, applied in the application, preparation of organic fertilizers, bacteria, etc., can solve the problems of unsatisfactory deodorization effect of deodorizing microorganisms, reduce the emission of odorous gases, and have broad application prospects. The effect of increasing the maturity

Pending Publication Date: 2022-04-29
山东蔚蓝生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people have been trying to improve and optimize new methods and approaches for screening deodorizing microorganisms. Due to the limitations of the screening method and the specificity of microorganisms, the deodorizing effect of the deodorizing microorganisms screened is not ideal. Therefore, screening for efficient deodorizing microbes are important

Method used

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  • Bacillus subtilis and application thereof in manure compost deodorization
  • Bacillus subtilis and application thereof in manure compost deodorization
  • Bacillus subtilis and application thereof in manure compost deodorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening of deodorizing bacterial strains

[0034] 1. Sample

[0035] Collected from broiler waste in Penglai, Yantai.

[0036] 2. Enrichment culture and purification of bacterial strains

[0037] Take 1g of chicken manure and add it to a culture bottle containing 99mL of sterile water, and add a few glass beads to make a suspension, then take 1mL from the suspension and put it into a culture bottle containing 50mL of enriched medium. Seal the seal and place it in a biochemical incubator at 30°C for static culture for 3-7 days. The enriched culture produces a lot of gas and has high turbidity, spread it on the separation medium, culture at 30°C for 2 days, pick out a single colony on the nutrient agar medium, and continue to purify 2 to 3 times. The purified single colonies were inoculated into 50 mL LB liquid medium respectively, and cultured at 37°C and 220 r / min for 14 hours. The number of effective viable bacteria in the fermentation broth of each str...

Embodiment 2

[0052] Identification of embodiment 2 YBS01 bacterial strain

[0053] 2.1 Identification of colony morphology

[0054] The colony morphology of YBS01 strain is as follows figure 1 As shown, the colony is light yellow, with a diameter of 5-57 mm, the colony has rough and opaque edges, and is flat in the middle. The spores are oval, partially round, and the sporangia do not expand. Cells exist alone, in pairs, or in short chains.

[0055] 2.2 16S rDNA molecular identification

[0056] The genome of YBS01 strain was extracted using a kit. Then using the genome as a template, the 16S rDNA was amplified using specific primers. The amplified PCR product was detected by 1% agarose gel electrophoresis and sent to a sequencing company for sequencing.

[0057] Sequencing results showed that the sequence of the PCR amplification product was SEQ ID NO:1. By comparing the sequence with BLAST in the NCBI database, it was found that it has the highest similarity with Bacillus subtilis...

Embodiment 3

[0064] Example 3 Evaluation of Bacillus subtilis YBS01 Enzyme Production Ability

[0065] 1. Preparation of bacteria solution

[0066] The activated Bacillus subtilis YBS01 was inoculated in LB liquid medium, cultured at 37°C and 220r / min for 14h, and the viable count was 10 8 -10 9 CFU / ml of bacterial liquid.

[0067] 2. Evaluation of Protease Production Ability

[0068] Spot the Bacillus subtilis YBS01 bacterial liquid on the agar medium containing skimmed milk, and cultivate it at 37°C for 48 hours. It can be seen that a transparent circle is formed around the Bacillus subtilis YBS01, and the diameter of the transparent circle is 25mm. This shows that Bacillus subtilis YBS01 can produce certain proteases.

[0069] Further, the Bacillus subtilis YBS01 bacterial liquid was centrifuged at 4° C. and 12000 rpm for 5 minutes, and the supernatant was obtained; the enzyme activity of protease in the fermentation supernatant of Bacillus subtilis YBS01 was detected by the following...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a novel bacillus subtilis strain and an application of the novel bacillus subtilis strain in manure compost deodorization. The bacillus subtilis is screened from showerhead broiler chicken breeding waste in Yitai City of Shandong Province, has a fixing effect on organic nitrogen and sulfur elements in compost raw materials, can effectively reduce release of odor such as ammonia gas and hydrogen sulfide, accelerates the manure composting process, improves the quality of organic fertilizer, and is suitable for large-scale production. The strain is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on January 13, 2021, and the preservation number is CCTCC NO: M2021053.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a novel strain of Bacillus subtilis and its application in manure composting and deodorization. Background technique [0002] With the continuous improvement of people's living standards, the demand for livestock and poultry products continues to increase. According to the data of the United Nations Food and Agriculture Organization (FAO), the world's demand for meat will increase by 73% by 2050. To meet the demand for meat , the animal husbandry needs to develop rapidly from the traditional free-range breeding to the direction of intensification and scale, followed by a large amount of livestock and poultry manure that has become an important source of environmental pollution. While a large amount of livestock and poultry manure is piled up and occupies land, the harmful gas produced pollutes the environment, seriously endangers human health, and affec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F3/00C05F17/20C05F17/80C12R1/125
CPCC12N1/20C12N1/16C12N1/14C05F3/00C05F17/20C05F17/80Y02A40/20Y02W30/40
Inventor 周英俊焦绪勇凌红丽苑伟伟陈琳孙文丽赵凯张春玉吴松涛
Owner 山东蔚蓝生物科技有限公司
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