A kind of medicament composition and application thereof that can be used for preventing and treating tobacco root black rot
A technology of root black rot and composition, which is applied in the field of pesticides to achieve the effects of slowing down drug resistance and reducing application doses
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Embodiment 1
[0015] Embodiment 1 sodium selenite is to the mycelia inhibitory action of tobacco root black rot fungus
[0016] Test method: using the toxic medium method, the tobacco root black rot fungus was activated and inoculated on PDA, and cultivated for 10 days until the mycelia covered the surface of the medium for later use. Accurately weigh the medicament, prepare sodium selenite into concentrations of 1ug / mL, 10ug / mL, 100ug / mL, 1000ug / ml and 10000ug / ml respectively, and use the commonly used antifungal agents carbendazim, prochloraz, pyrazole Azoxystrobin was configured so that the above concentration was used as a positive control, and sterile water was used as a negative control, and it was sterilized under an ultraviolet lamp for later use. Prepare the culture medium according to the ratio of medicine to culture medium 1:10, take out 1m L of medicine solution in a petri dish with a pipette gun, then take 9mL of melted potato agar medium and mix evenly, so that sodium selenite...
Embodiment 2
[0020] Example 2 Sodium Selenite Compound Composition Effects on Mycelia Growth of Tobacco Root Black Rot Fungus
[0021] According to the antibacterial effect of the above agents, sodium selenite, carbendazim, prochloraz and pyraclostrobin were respectively mixed according to the ratio of 1:1, 2:3, 1:4, 1:10, 1:20 For compounding. The growth rate method was used for the test, referring to the method of fungicide single agent, the toxicity regression equation was drawn by DPS data processing, and the EC was calculated. 50 . Then calculate the co-toxicity coefficient (CTC) according to Sun Yunpei's method. When CTC≤80, the composition exhibits antagonistic effect; when 80<CTC<120, the composition exhibits additive effect; when CTC≥120, the composition exhibits synergistic effect.
[0022] Actual Toxicity Index (ATI) = (standard drug EC50 / test drug EC50) × 100
[0023] Theoretical toxicity index (TTI) = Toxicity index of agent A × percentage of A in the mixture + toxicity in...
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