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Trichoderma virens with functions of preventing diseases and promoting growth and application of trichoderma virens

A kind of Trichoderma viridans, functional technology, applied in the field of biological control, can solve the problem of less biological control strains, etc., to achieve the effect of environmental friendliness

Active Publication Date: 2020-05-12
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The currently reported biocontrol strains related to tobacco root rot mainly include Bacillus, actinomycetes and Trichoderma and other strains, while there are few studies on tobacco root rot biocontrol bacteria, which can simultaneously control tobacco root black rot and There are fewer biocontrol strains of root rot, so it is of great theoretical and practical significance to screen Trichoderma that can effectively control these two diseases and have a growth-promoting effect on tobacco

Method used

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  • Trichoderma virens with functions of preventing diseases and promoting growth and application of trichoderma virens
  • Trichoderma virens with functions of preventing diseases and promoting growth and application of trichoderma virens
  • Trichoderma virens with functions of preventing diseases and promoting growth and application of trichoderma virens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the screening of biocontrol bacteria (plate antagonism test)

[0043] The soil was collected in the sesame field of Pingyu County, Zhumadian City, Henan Province. After grinding and sieving, 5g of the soil sample was taken, added to a triangular flask filled with 45mL of sterile water, shaken on a shaking table at 160r / min for 30min, left to stand for 30s, and the supernatant was taken. The stock solution was diluted 10 times to 10 -4g / mL. Take out 100 μL of the diluted solution and spread it on the Bengal red medium plate, incubate at 28°C, isolate and purify the colony after it grows out, pick the edge of the colony and purify it on a new PDA plate, and store the purified strain in 25% glycerol , stored at -70°C for later use.

[0044] The purified fungi were screened by the plate confrontation method and the drug-containing plate method with the target fungus of Trichomonas rhizogenes and Fusarium oxysporum.

[0045] Plate confrontation method Inocul...

Embodiment 2

[0053] Embodiment 2, the antibacterial spectrum determination of biocontrol bacteria

[0054] The antibacterial spectrum of NKSF2019001F was determined by plate confrontation and drug-containing plate method, respectively.

[0055] Concrete method is by the method of embodiment 1. The results are shown in Table 3 and figure 2 , image 3 .

[0056] From Table 3 and figure 2 , image 3 It can be seen that in the plate confrontation test, bacterial strain NKSF2019001F has good antibacterial activity to 12 kinds of pathogenic bacteria tested, and the average bacteriostatic rate of 12 kinds of pathogenic bacteria reaches 61.67%-98.99%. the highest antibacterial rate (98.99%), followed by Rhizoctonia graminearum (98.40%); Except for Botrytis and Botrytis, the bacteriostatic rate to other 7 kinds of pathogenic bacteria was more than 50%, and the bacteriostatic rate to Helminthosporium soxhleti was the highest (76.47%). It shows that the isolate NKSF2019001F has good broad-sp...

Embodiment 3

[0059] Embodiment 3, identification of biocontrol bacteria

[0060] The results of morphological observation showed that the NKSF2019001F colony was round on the PDA plate, and the mycelium was white in the initial stage of growth, with aerial hyphae. After 2 days of cultivation at 25°C, amorphous cotton-like white aerial mycelia were produced, and meristems began to form. Spores, in the later stage, obvious piles of conidia appeared, converging into wide gray-green concentric ring patterns ( Figure 4 ). Observed under a microscope, the hyphae are colorless and slender, separated, and multi-branched; conidiophores grow from the lateral branches of the mycelium, generally with 2-3 whorled branches, bottle-shaped ampoule-shaped, solitary or opposite , verticillate arrangement ( Figure 5 A). Most conidia are oval or subspherical with smooth walls ( Figure 5 B).

[0061] Using the genome of the biocontrol Trichoderma strain NKSF2019001F as a template, its ITS fragment was ...

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Abstract

The invention relates to trichoderma virens capable of preventing both tobacco black root rot and tobacco root rot and promoting tobacco growth, and belongs to the technical field of biological prevention and control. The trichoderma virens NKSF2019001F has been collected in China Center for Type Culture Collection, and the collection number of the trichoderma virens is CCTCC NO: M2019767; and theinvention further provides a screening method for the trichoderma virens and a biocontrol bacterium solution of the trichoderma virens. The strain can control both the tobacco black root rot and thetobacco root rot, and the control effect of the tobacco black root rot and the control effect of the tobacco root rot both reach 65% or more; the strain can effectively reduce the incidence rate of tobacco root and stem diseases, and is equivalent to existing common chemical pesticides in control effect; meanwhile, the strain further has the function of promoting the tobacco growth; and after being used, the trichoderma virens cannot generate resistance, cannot cause the problems of pesticide residues, environmental pollution and the like, is safe and environmental-friendly, and meets the requirement of ecological agriculture.

Description

technical field [0001] The invention relates to a Trichoderma viride capable of simultaneously preventing and controlling tobacco root black rot and root rot and promoting tobacco growth, and belongs to the technical field of biological control. Background technique [0002] Tobacco pests and diseases are one of the main factors restricting the high-quality and stable production of tobacco, especially tobacco rhizome diseases are complex and harmful. For a long time, tobacco rhizome diseases have caused tobacco production reduction, and caused devastating losses when serious. In recent years, tobacco root black rot and tobacco root rot often occur together in Henan smoking areas, causing huge losses to tobacco farmers. [0003] Tobacco root black rot is a common soil-borne fungal disease, and its pathogen is Thielaviopsis basicola. Root black rot can occur in tobacco from the seedling stage to the adult plant stage. It mainly infects the roots of tobacco. When the disease ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12Q1/18C12Q1/04A01N63/38A01P21/00A01P3/00C12R1/885
CPCC12N1/14C12N1/02C12Q1/18C12Q1/04C12R2001/885C12N1/145
Inventor 刘红彦千慧敏赵辉刘新涛倪云霞文艺李小杰李淑君赵新贝何碧珀
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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