CsALKBH8 and its coding gene are applied to regulating postharvest disease resistance of citrus fruits

By transiently overexpressing the CsALKBH8 gene in citrus fruits to regulate their disease resistance, the problem of postharvest rot in citrus fruits was solved, achieving green and efficient disease control and providing important gene resources.

CN122405583APending Publication Date: 2026-07-17SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Patent Information

Application Number
CN202610673483.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-15
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies, citrus fruits are susceptible to green mold disease caused by Penicillium finger infection after harvest, leading to rot. Traditional chemical control methods have problems such as environmental pollution and pathogen resistance, and lack green and efficient disease control strategies.

Method used

By screening and identifying the CsALKBH8 gene and its encoded protein, the gene was transiently overexpressed in citrus fruits using an Agrobacterium-mediated method to regulate the disease resistance of the fruits and enhance their resistance to Penicillium digitatum infection.

Benefits of technology

It significantly reduced the incidence and diameter of lesions on the fruit, increased the expression level of disease resistance-related genes, provided a green and efficient means of disease control, and provided genetic resources for the breeding of superior citrus fruit varieties.

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Abstract

本发明公开了CsALKBH8及其编码基因在调控柑橘果实采后抗病性中的应用。柑橘RNA去甲基化酶CsALKBH8的氨基酸序列如SEQ ID NO.2所示,其基因的核苷酸序列如SEQ ID NO.1所示。本发明首次鉴定了CsALKBH8的功能,该基因在柑橘果皮中能够快速响应指状青霉的侵染,且表达水平明显增加,与果实抗病性正相关。CsALKBH8定位于细胞核和细胞质膜,将该基因在柑橘果皮上瞬时过表达,降低柑橘果实绿霉病的发病率和病斑直径,表明CsALKBH8基因及其编码的蛋白质可以调控果实的抗病能力。该基因的发掘与利用为抗病果实的培育研究提供了基因资源,对柑橘果实基因育种和病害防治具有重要意义和应用前景。
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