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Method for preparing enteromorpha extract for inhibiting red tide algae and its use

A technology for extracting liquid and red tide algae, which is applied in the field of preparation of Enteromorpha extract, can solve the problems of aquaculture loss, reduce seawater dissolved oxygen, aquatic animals and human health hazards, and achieve good ecological security and easy operation and control , the effect of inhibiting growth

Inactive Publication Date: 2006-10-25
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eutrophication leads to the explosive growth of red tide algae, which will reduce the dissolved oxygen in seawater and bring huge losses to the aquaculture industry
Some algae can also produce algae toxins, which are harmful to aquatic animals and human health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Soak 0.1 kg of Enteromorpha edulis in 3 liters of sterilized seawater for 2 days, then filter, and concentrate the filtrate to 100 ml. Take concentrated solution 1, 2, 3, 4, 5 milliliters and add respectively to 200 milliliters of Skeletalum costa culture medium, after 5 days, inhibition rate is respectively 42%, 78%, 94%, 97%, 99%.

Embodiment 2

[0014] After extracting 1 kg of Enteromorpha spp. with 2 liters of ethanol for 12 hours, 5 ml of the obtained extract was applied to 200 ml of culture medium of Skeletalum costanum. After 5 days, the algae density was only 3% of that of the control group, that is, the inhibition rate was 97%. %.

[0015] After extracting 1 kg of Enteromorpha spp. with 2 liters of ethanol for 48 hours, 5 ml of the obtained extract was applied to 200 ml of culture medium of Skeletalum costanum. After 5 days, the algae density was only 2.5% of that of the control group, that is, the inhibition rate was 97.5%. %.

Embodiment 3

[0017] After extracting 1 kg of Enteromorpha edulis with 2 liters of acetone for 12 hours, 5 milliliters of the obtained extract was applied to 200 milliliters of Skeletalum costa culture medium. After 5 days, the algae density was only 5% of that of the control group, that is, the inhibition rate was 95%. %.

[0018] After extracting 1 kg of Enteromorpha edulis with 2 liters of acetone for 24 hours, 5 milliliters of the obtained extract was applied to 200 milliliters of Skeletalum costa culture medium. After 5 days, the algae density was only 2% of that of the control group, that is, the inhibition rate was 98%. %.

[0019] After extracting 1 kg of Enteromorpha spp. with 2 liters of acetone for 48 hours, 5 ml of the obtained extract was applied to 200 ml of culture medium of Sclerotina costarum. After 5 days, the algae density was only 1.5% of that of the control group, that is, the inhibition rate was 98.5% %.

[0020] After extracting 1 kg of Enteromorpha spp. with 5 lite...

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PUM

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Abstract

The invention relates to a method for preparing Enteromorpha prolifera extract for prohibiting red tide alga and the application thereof. And the preparing method: leaching Enteromorpha linza plants or their crushed matters with water, alcohol or acetone, aether, and chloroform or methanol, and concentrating the obtained extract by rotary evaporation, and throwing the concentrated extract into a to-be-processed water body to prohibit growth of red tide alga in the water body. And the extract of the Enteromorpha linza extract can be used to prohibit the red tide alga growing. Ordinarily, per kg Enteromorpha linza is leached with 30-50 L sterilized sea water for 1-3 days and the obtained Enteromorpha linza extract is applied to the to-be-processed water body, and 10-100 ml extract is thrown into per L water body and the prohibition rate is above 98%. And the invention can effectively prohibit harmful marine red tide alga.

Description

technical field [0001] The invention belongs to the field of water treatment, in particular to a preparation method and application of Enteromorpha extract for inhibiting red tide algae. Background technique [0002] Nutrients from land sources and pollutants discharged and leaked from marine operations enter the ocean water body, leading to eutrophication of seawater, which is the main reason for the deterioration of the marine environment. Eutrophication leads to the explosive growth of red tide algae, which will reduce the dissolved oxygen in seawater and bring huge losses to the aquaculture industry. Some algae can also produce algae toxins, which are harmful to aquatic animals and human health. S. costata is a common and main red tide algae in the east coast of China, so S. costata is usually used as a representative of red tide algae. [0003] Existing red tide inhibitors are mainly copper ion preparations and synthetic organic herbicides. Copper ions (Cu 2+ ) Kill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/00C02F3/00C02F3/32
Inventor 李锋民王震宇安蓁
Owner OCEAN UNIV OF CHINA
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