Method for extracting allelopathy refinement component having algae inhibition activity from barley by using ethyl acetate

A technology of algae-inhibiting activity and ethyl acetate, applied in botany equipment and methods, chemicals for biological control, applications, etc. Unable to inhibit the growth of algae and other problems, achieve the effect of good algae inhibition, low price, and good ecological security

Active Publication Date: 2013-02-13
ZHEJIANG UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the patent applied by the applicant in 2011 (patent publication number: CN102415416A, titled: "A method for preparing a new type of algal bloom inhibitor from barley"), it is pointed out that the chloroform and ethyl acetate extracts of barley straw have obvious algae-inhibiting activity, but the patent did not further separate the active components of the extract, and failed to clearly explain the main algae-inhibiting substances. Since the proportion of the main algae-inhibiting components in the extract is unknown, it cannot be used in practical applications. Quantitatively determine the appropriate amount of use, which may cause waste of chemicals or insufficient use to inhibit the growth of algae
It will cause certain difficulties to develop corresponding algae inhibitors in the future

Method used

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  • Method for extracting allelopathy refinement component having algae inhibition activity from barley by using ethyl acetate
  • Method for extracting allelopathy refinement component having algae inhibition activity from barley by using ethyl acetate
  • Method for extracting allelopathy refinement component having algae inhibition activity from barley by using ethyl acetate

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Experimental program
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Effect test

Embodiment 1

[0025] Firstly, the barley straw was dried at 50° C. for 96 hours, and the dried barley was crushed to 20 meshes. Soak 400 g of dry barley with 2 L of aqueous ethanol with an alcohol content of 75%, extract 5 times at 20 °C for 2 hours each time, and combine to obtain barley alcohol extract; In the rotary evaporator, the solvent is distilled off under reduced pressure to obtain extract A; the extract A is extracted twice with 1-1.5 times of ethyl acetate with a volume of water successively, and extract B is obtained after concentrating the ethyl acetate phase extract. The HPLC spectrum of ointment B sees figure 2 ;

[0026] Secondly, the extract B was subjected to silica gel column chromatography, and the eluents were dichloromethane and methanol, and the volume ratios were 100:0, 49:1, 19:1, 10:1, 3:2, 4:1 and The gradient of 0:100 was eluted, the eluent was collected and the solvent was removed, and the anti-algae activity of each eluted component was tested. The results ...

Embodiment 2

[0031] Firstly, the barley straw was dried at 70° C. for 48 hours, and the dried barley was crushed to 50 mesh. Soak 1 Kg of dry barley with 4.5 L of ethanol containing 40% alcohol, extract 2 times at 80°C for 6 hours each time, and combine to obtain the barley alcohol extract; put the barley alcohol extract at 50°C under reduced pressure in a rotary evaporator to remove the solvent to obtain extract A; sequentially extract extract A with an appropriate amount of ethyl acetate four times, and concentrate the ethyl acetate phase extract to obtain extract B;

[0032] Secondly, the extract B was subjected to silica gel column chromatography, and the eluent was dichloromethane: methanol with a gradient of 100:0, 49:1, 19:1, 10:1, 3:2 to 0:100. The eluate was collected respectively and the solvent was removed, and the algae-inhibiting activity was tested for each eluted component, and the results are shown in Table 2:

[0033] eluent component ratio Algae inhibition ...

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Abstract

The present invention discloses a method for extracting an allelopathy refinement component having algae inhibition activity from barley by using ethyl acetate. According to the method, barley straws are subjected to a pretreatment and extraction separation based on polarity to obtain an ethyl acetate phase having algae inhibition activity, and the obtained extraction solution is completely dried and separated by using a positive direction silica gel column to obtain the refinement component having strong algae inhibition ability, wherein an eluant is prepared by mixing dichloromethane and methanol according to a volume ratio of 49:1. The method has characteristics of simpleness, easy performing, low cost, wide and easily-available materials and the like. With the method, the high purity barley allelopathy component having the algae inhibition activity can be obtained, and can be widely applicable for control of algae water blooms in lakes and reservoirs.

Description

technical field [0001] The invention belongs to the field of water body pollution control, and particularly relates to a method for extracting algae-inhibiting subdivided components of ethyl acetate phase from barley. Background technique [0002] In recent years, China's environmental problems have become increasingly serious, one of which is the eutrophication of water bodies. The eutrophication of the water body will lead to the massive growth of algal blooms, reduce the transparency of the water body, reduce the dissolved oxygen, lead to the death and corruption of a large number of aquatic organisms, and eventually cause the rapid collapse of the aquatic system, causing serious ecological damage and huge economic losses. [0003] At present, algae control technology can be roughly divided into physical technology, chemical technology and biological technology, the most commonly used is chemical technology. A large number of studies have shown that the reproduction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/44A01P13/00
Inventor 肖溪陈英旭黄皓旻
Owner ZHEJIANG UNIV
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