Method and application for extracting alexandrium tamarense inhibitor from enteromorpha
A technology of inhibitors and red tide algae, applied in the field of seawater treatment, can solve the problems of marine ecological environment loss, seawater eutrophication, aquatic animals and human health hazards, and achieve good ecological security, growth inhibition, and easy operation and control Effect
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Example Embodiment
[0016] Example 1
[0017] Soak 0.1 kg of Enteromorpha in 0.3 liters of distilled water for 24 hours, filter, and concentrate the filtrate.
[0018] Dissolve the concentrated paste with 40 ml of a 1:1 mixture of petroleum ether and 90% ethanol. Shake well, let stand for stratification, and remove the lower layer (ethanol phase). The ethanol phase was concentrated by evaporation under reduced pressure at 40°C to obtain a paste, which was extracted and separated with 20 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (the amount obtained is about 10 ml).
[0019] 0.1, 0.2, 0.3, 0.4 and 0.5 ml of inhibitor were added to 200 ml of Gymnodon brevis culture solution respectively. After 3 days, the inhibition rates were 65%, 86%, 96%, 98% and 99.5%, respectively.
Example Embodiment
[0020] Example 2
[0021] 1 kg of prolifera was soaked in 2 liters of distilled water for 48 hours, filtered, and the filtrate was concentrated.
[0022] Dissolve the concentrated cream with 100 ml of a 1:1 mixture of petroleum ether and 90% ethanol. Shake well, let stand for stratification, and remove the lower layer (ethanol phase). The ethanol phase was concentrated by evaporation under reduced pressure at 40°C to obtain a paste, which was extracted and separated with 100 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (the amount obtained is about 50 ml).
[0023] 0.2, 0.3, 0.4, 0.5 and 1 ml of inhibitor were added to 100 ml of Gymnodon brevis culture solution respectively. After 3 days, the inhibition rates were 81%, 92%, 97%, 99% and 99%, respectively.
Example Embodiment
[0024] Example 3
[0025] 1 kg of prolifera was soaked in 5 liters of distilled water for 24 hours, filtered, and the filtrate was concentrated.
[0026] Dissolve the concentrated cream with 100 ml of a 1:1 mixture of petroleum ether and 90% ethanol. Shake well, let stand for stratification, and remove the ethanol phase of the lower layer. The ethanol phase was concentrated by evaporation under reduced pressure at 40°C to obtain a paste, which was extracted and separated with 100 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (the amount obtained is about 50 ml).
[0027] 0.2, 0.3, 0.4, 0.5 and 1 ml of inhibitor were added to 100 ml of Gymnodon brevis culture solution, respectively. After 2 days, the inhibition rates were 76%, 82%, 87%, 95% and 97%, respectively.
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