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Method and application for extracting alexandrium tamarense inhibitor from enteromorpha

A technology of inhibitors and red tide algae, applied in the field of seawater treatment, can solve the problems of marine ecological environment loss, seawater eutrophication, aquatic animals and human health hazards, and achieve good ecological security, growth inhibition, and easy operation and control Effect

Inactive Publication Date: 2010-09-29
秦旭坤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to land-based nutrients and pollutants discharged and leaked from marine operations, a large amount of pollutants enter the ocean, resulting in eutrophication of seawater
Eutrophication leads to the explosive growth of red tide algae, which brings huge losses to the marine ecological environment, aquaculture industry, tourism industry, etc.
Some algae can also produce algae toxins, which are harmful to aquatic animals and human health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 0.1 kg of Enteromorpha and soak it in 0.3 liter of distilled water for 24 hours, then filter it, and concentrate the filtrate.

[0018] Dissolve the concentrated paste with 40 ml of 1:1 mixture of petroleum ether and 90% ethanol. Vibrate fully, let stand to separate layers, and remove the lower layer (ethanol phase). The ethanol phase was evaporated and concentrated under reduced pressure at 40°C to obtain a paste, which was separated by extraction with 20 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (acquisition volume is about 10 ml).

[0019] Add 0.1, 0.2, 0.3, 0.4, 0.5 ml of inhibitors to 200 ml of typical red tide alga Pyrodinophyllum culture solution respectively, after 3 days, the inhibitory rates are 65%, 86%, 96%, 98%, 99.5%.

Embodiment 2

[0021] 1 kg of Enteromorpha was soaked in 2 liters of distilled water for 48 hours, then filtered, and the filtrate was concentrated.

[0022] Dissolve the concentrated paste with 100 ml 1:1 mixture of petroleum ether and 90% ethanol. Vibrate fully, let stand to separate layers, and remove the lower layer (ethanol phase). The ethanol phase was evaporated and concentrated under reduced pressure at 40°C to obtain a paste, which was separated by extraction with 100 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (acquisition volume is about 50 ml).

[0023] Add 0.2, 0.3, 0.4, 0.5, and 1 ml of the inhibitors into 100 ml of the typical red tide algae Pyrodinophyllum culture solution. After 3 days, the inhibitory rates were 81%, 92%, 97%, 99%, and 99%, respectively.

Embodiment 3

[0025] 1 kg of Enteromorpha was soaked in 5 liters of distilled water for 24 hours, then filtered, and the filtrate was concentrated.

[0026] Dissolve the concentrated paste with 100 ml 1:1 mixture of petroleum ether and 90% ethanol. Vibrate fully, let stand to separate layers, and remove the ethanol phase of the lower layer. The ethanol phase was evaporated and concentrated under reduced pressure at 40°C to obtain a paste, which was separated by extraction with 100 ml of 1:1 ethyl acetate and double distilled water. The ethyl acetate phase is the red tide algae inhibitor (acquisition volume is about 50 ml).

[0027] Add 0.2, 0.3, 0.4, 0.5, and 1 ml of the inhibitors to 100 ml of the typical red tide alga Pyrodinophyllum culture solution. After 2 days, the inhibitory rates were 76%, 82%, 87%, 95%, and 97%.

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PUM

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Abstract

The invention relates to a method and an application for extracting an alexandrium tamarense inhibitor from enteromorpha. The method comprises the following steps of: lixiviating per kilogram of the enteromorpha by using 2-5 liters of distilled water at room temperature for 12-72 hours, filtering to take filter liquor, and concentrating the obtained filter liquor into pastes by adopting a rotary evaporation method; dissolving the concentrated pastes by using a mixing solution of petroleum ether and 90 percent of alcohol which are in a 1:1 ratio; sufficiently oscillating for 1-3 minutes, then demixing by standing for 10-120 minutes, and taking a lower-layer alcohol phase; decompressing, evaporating and concentrating the alcohol phase to obtain a paste-like substance at 40 DEG C; extractingand separating by using ethyl acetate and secondary distilled water which are in the 1:1 ratio, wherein a ethyl acetate phase is the alexandrium tamarense inhibitor; and determining the volume of thealexandrium tamarense inhibitor extracted by per kilogram of the enteromorpha to 50 milliliters. The invention is used for inhibiting the growth of alexandrium tamarense by adding 0.5-5 milliliters of the alexandrium tamarense inhibitor to per cubic metre of a water body, sufficiently utilizes the enteromorpha, has efficient economy, good ecological safety and easy operation and control, effectively inhibits the growth of the oceanic alexandrium tamarense and particularly inhibits the growth of gymnodinium breve.

Description

technical field [0001] The invention belongs to the field of seawater treatment, in particular to a method and application for extracting red tide algae inhibitors from enteromorpha. Background technique [0002] Enteromorpha prolifra (Muell.) J.Ag., also known as moss bar and moss, is a common large seaweed. The algal body is grass-green, tubular and membranous, clustered, with obvious main branches and slender branches, up to 1 meter high. Since 2006, a large number of Enteromorpha have gathered and floated on the surface of the Yellow Sea every summer, forming large-scale green tides in northern Jiangsu and the southern coast of Shandong Peninsula. Especially in June 2008, a large amount of Enteromorpha drifted from the central Yellow Sea to the waters near Qingdao, and the coastal waters and coasts of Qingdao encountered a sudden and rare natural disaster of Enteromorpha. At its peak, the distribution area reached 24,000 square kilometers. The 60-square-kilometer sea ...

Claims

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Application Information

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IPC IPC(8): A01N65/03A01P1/00C02F1/50
Inventor 秦旭坤李锋民
Owner 秦旭坤
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