Method for extracting allelopathic subdivided component having anti-algal activity from barley with petroleum ether

A technology of algae inhibitory activity and petroleum ether, which is applied in the field of extracting allelopathic sub-components with algae inhibitory activity, can solve the problem that it is impossible to determine whether there are substances that promote algal growth or some unknown substances, increase the risk of use, and separate active components, etc. problem, to achieve the effect of low price, good ecological safety and low preparation cost

Active Publication Date: 2012-10-24
ZHEJIANG UNIV
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Problems solved by technology

[0005] The applicant applied for a patent in 2011 (patent publication number: CN102415416A, titled: "A method for preparing a new type of algal bloom inhibitor from barley"), which pointed out that the extraction of barley straw with petroleum ether, chloroform and ethyl acetate The liquid has obvious anti-algae activity, but the active ingredients of the extract are not further separated in the patent. Since the components in the plant extract are relatively complex, it is impossible to determine whether there are substances or some unknown substances that promote the growth of algae. Increase the risk of use and cause certain difficulties for the development of corresponding algicides in the future

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  • Method for extracting allelopathic subdivided component having anti-algal activity from barley with petroleum ether

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Embodiment 1

[0021] Firstly, the barley straw was dried at 50° C. for 96 hours, and the dried barley was crushed to 20 meshes. Infiltrate 400 grams with 2L of aqueous ethanol with an alcohol content of 75%, extract 5 times at 20°C, and extract 2 hours each time, and combine to obtain the barley alcohol extract; put the barley alcohol extract at 40°C in a rotary evaporator Medium and reduced pressure distillation to remove the solvent to obtain the extract A; extract the extract A twice with an appropriate amount of petroleum ether, and concentrate the petroleum ether phase extract to obtain the extract B;

[0022] Secondly, the extract B was subjected to silica gel column chromatography, eluted with a gradient of petroleum ether: ethyl acetate at 100:0, 19:1, 10:1, 4:1, 3:2 to 0:100, and collected Eluent, carries out anti-algae activity test, and the results are as shown in table 1:

[0023] Table 1: Components of petroleum ether phase eluent and their anti-algae effects

[0024] ...

Embodiment 2

[0027] Firstly, the barley straw was dried at 70° C. for 48 hours, and the dried barley was crushed to 50 mesh. Soak 1Kg of dried barley with 4.5L of 40% ethanol, extract twice at 80°C for 6 hours each time, and combine to obtain the barley alcohol extract; put the barley alcohol extract at 50°C in Remove the solvent by distillation under reduced pressure in a rotary evaporator to obtain extract A; extract A with an appropriate amount of petroleum ether, extract four times, and concentrate the petroleum ether phase extract to obtain extract B;

[0028] Secondly, the extract B was subjected to silica gel column chromatography, eluted with a gradient of petroleum ether: ethyl acetate at 100:0, 19:1, 10:1, 4:1, 3:2 to 0:100, and collected Eluent, carries out anti-algae activity test, and the results are as shown in table 2:

[0029] Table 2: Petroleum ether phase eluent composition and its algae inhibition effect

[0030] eluent component ratio Algae inhibition ra...

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Abstract

The invention discloses a method for extracting an allelopathic subdivided component having an anti-algal activity from barley with petroleum ether. The method comprises the steps of pre-treating barley straws and extracting for separation based on polarity, and separating to obtain a petroleum ether phase having the anti-algal activity; drying the obtained extracting solution absolutely; then separating with a normal-phase silica column, wherein an eluent is composed by mixing petroleum ether and ethyl acetate according to a volume ratio of 10:1; and thus the allelopathic subdivided component with relatively strong anti-algal activity is obtained. The method has the characteristics of simple and practicable operation, low cost and wide materials. With the method, the allelopathic subdivided component with relatively strong anti-algal activity can be obtained, and the allelopathic subdivided component can be widely used for controlling algal blooms in lakes and reservoirs.

Description

technical field [0001] The invention belongs to the field of water body pollution control, and particularly relates to a method for extracting allelopathic subdivided components with algae-inhibiting activity from barley with petroleum ether. Background technique [0002] In recent years, the problem of environmental pollution in China has become more and more serious. A large amount of nitrogen, phosphorus and other nutrient pollutants directly enter the water body, causing the imbalance of the water body ecosystem. Cyanobacteria blooms occur in large-scale freshwater lakes due to eutrophication. The toxins produced by the outbreak of cyanobacteria blooms will seriously threaten the safety of urban drinking water supply. [0003] The most fundamental algae control method achieves the purpose of fundamentally inhibiting the growth of algae by reducing the nutrients entering the water body, but it is difficult to implement at present, and it cannot solve the problem of endog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/44A01P13/00
Inventor 肖溪陈英旭黄皓旻
Owner ZHEJIANG UNIV
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