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An HPLC Analytical Method for Simultaneous Determination of 14 Kinds of Vitamins

A high-performance liquid chromatography and analysis method technology, which can be applied to measurement devices, analysis materials, material separation, etc., can solve the problems of incomplete detection of vitamin types, cumbersome and time-consuming operations, etc. Easy to use effect

Active Publication Date: 2017-10-03
CHONGQING FANGTONG ANIMAL PHARMA
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  • Abstract
  • Description
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Problems solved by technology

However, due to the wide variety of vitamins, they are sensitive to temperature, humidity, light and other conditions. The most important thing is that the solubility of each vitamin is not the same. The existing methods for content determination are mostly based on the determination of a single vitamin. Disadvantages such as cumbersome and time-consuming operation
There are also reports in the literature that methods for simultaneously determining the contents of several fat-soluble vitamins or water-soluble vitamins still have shortcomings such as incomplete detection of vitamin types. vitamin) content method

Method used

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  • An HPLC Analytical Method for Simultaneous Determination of 14 Kinds of Vitamins
  • An HPLC Analytical Method for Simultaneous Determination of 14 Kinds of Vitamins
  • An HPLC Analytical Method for Simultaneous Determination of 14 Kinds of Vitamins

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Embodiment Construction

[0043] In order to make the object, technical solution and beneficial effect of the present invention clearer, preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0044] 1. Content determination method

[0045] 1. Chromatographic conditions

[0046] Chromatographic column: C18 chromatographic column (250mm×4.6mm, 5µm);

[0047] Mobile phase and elution method: use 0.1M sodium acetate solution as mobile phase A, methanol as mobile phase B, and perform gradient elution according to the following procedure:

[0048] 0-4min: mobile phase is mobile phase A-mobile phase B (volume ratio 95: 5);

[0049] 4-10min: The mobile phase changes linearly from mobile phase A-mobile phase B (volume ratio 95:5) to mobile phase A-mobile phase B (volume ratio 55:45);

[0050] 10-18min: The mobile phase changes linearly from mobile phase A-mobile phase B (volume ratio 55: 45) to mobile phase A-mobile phase B (volume ra...

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Abstract

The invention discloses a high performance liquid chromatographic analysis method for simultaneous determination of the content of 14 kinds of vitamins; octadecylsilane bonded silica gel is used as a filling agent, a 0.1 M sodium acetate solution-methanol is used as a mobile phase for gradient elution, gradient flow velocity of the mobile phase and a program change wavelength of an ultraviolet detector are combined, and intact separation and quantitative analysis of the 14 kinds of vitamins (including 4 kinds of fat soluble vitamins and 10 kinds of water soluble vitamins) can be achieved in 60 minutes. The method has the advantages of strong specificity, high precision, good stability, high accuracy, simple operation, and cheap and easily available reagents and consumable materials, is suitable for quality control of compound vitamin products in medicine and food industries, and can effectively save the cost and time of detection.

Description

technical field [0001] The invention belongs to the technical field of analysis, and relates to a method for simultaneously determining the contents of 14 vitamins by using a high-performance liquid chromatography (HPLC) method. Background technique [0002] Vitamins can maintain the normal physiological functions and material and energy metabolism of living organisms, and are one of the important nutrients for humans and animals. According to their solubility, they can be divided into water-soluble vitamins (B vitamins and vitamin C) and fat-soluble vitamins (vitamin A, vitamin D, vitamin E, vitamin K). With the rapid development of the economy, the continuous improvement of people's living standards, and the gradual strengthening of health awareness, multivitamin products are becoming more and more popular in the market. Various multivitamin foods, health products, medicines, etc. emerge in endlessly. The variety is also increasing day by day. However, due to the wide va...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 唐建华龚聪毕海林潘瑶肖裕章胡军宋小红袁启峰何艳
Owner CHONGQING FANGTONG ANIMAL PHARMA
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