Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction

A solid-phase extraction column and cyclodextrin technology are used in the field of solid-phase extraction technology for separation and enrichment of chemical and chemical operations, which can solve problems such as inability to elute and achieve the effect of rapid detection.

Inactive Publication Date: 2007-12-26
SHANGHAI UNIV
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Problems solved by technology

Due to the inclusion effect of cyclodextrin, some compounds cannot be effectively eluted by common organic elution solvents such as methanol and acetonitrile

Method used

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  • Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction
  • Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction
  • Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction

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Embodiment 1

[0039] The instruments and experimental conditions adopted in this embodiment are as follows:

[0040] (1) Capillary electrophoresis instrument is a high-efficiency capillary electrophoresis system (CE-L1) produced by Singapore Pte Company, equipped with a UVIS 200 ultraviolet detector, and data acquisition and processing are completed by CSW chromatographic software.

[0041] (2) Micellar Electrokinetic Chromatography (MEKC) process, using the above capillary electrophoresis instrument. 20 mM SDS was prepared with 25 mM phosphate buffer (pH 8.0), then 5% acetonitrile was added, and the solution was filtered through a filter with a 0.45 micron pore size to obtain a MECK buffer solution. The pressure injection method (0.3psi) was adopted, the injection time was 10s, the separation voltage was 20kV, the detection wavelength was 214nm, and the experimental temperature was 25°C.

[0042] (3) β-cyclodextrin (β-CD), purchased from Sigma, USA; β-cyclodextrin bonded silica gel (CDS) ...

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Abstract

The invention relates to a solid phase extract method of using the diamond alkyl ramification as elution reagent ingredient which contains link dextrin solid phase extract load body, belongs to the related separation enrichment chemistry and the solid phase extract processing of the chemical operation technology domain. The step of the invention method is as follow: (1) activating the solid phase extraction column which contains the link dextrin load body, flushing the extraction column by methyl alcohol or two distilled water; (2) taking the sample, joining the sample solution in the solid phase extraction column, and forcing the solution into the fixed phase, and keeping the sample analysis on the fixed phase; (3) drip washing, with two distilled water and so on drip washings, washing off the sample component which does not need or abbreviates this step; (4) eluting reagent which contains the diamond alkane ramification through the solid phase extraction column, and eluting the wait analysis from the link dextrin solid phase extract load body; (5) examination analysis, the eluent which collects from the above directly carries on chromatogram, electrophoresis and analyses, then computing and analyzing the obtained detection curve atlas, or the collection eluent uses in other research work.

Description

technical field [0001] The invention relates to a solid-phase extraction method using an adamantane derivative as an elution reagent containing a cyclodextrin solid-phase extraction carrier, and belongs to the technical field of solid-phase extraction technology related to separation and enrichment chemistry and chemical operation. Background technique [0002] Solid phase extraction (SPE) is a new extraction technique that appeared in the 1970s, and is mainly used for the preparation of analytical samples. SPE has the following obvious advantages: simple, fast, matching SPE unit greatly shortens the analysis time, the processed samples are easy to store and transport, and it is convenient for quality control in different laboratories: not easy to emulsify; suitable for small volume samples Treatment; no need for a large amount of high-purity, flammable, and toxic solvents; there are many types of adsorbents and solvents to choose from, which can provide a variety of experim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/286
Inventor 王田霖王丹
Owner SHANGHAI UNIV
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