Method for extracting tripterine

A technology for triptolide and an extraction method, which is applied in the field of extraction of triptolide, can solve the problems of high cost, low content of triptolide in bitter melon, difficult industrialization and the like, and achieves the effect of low cost of raw materials

Inactive Publication Date: 2019-09-06
北京浩鼎瑞健康科技中心(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the industrial synthesis of tripterygium has many steps, the cost is high, and it is difficult to industrialize; a small amount of tripterygne exists in the roots of Tripterygium, but the roots and flowers of this genus also have many other toxic compounds. Taking some extracts of Tripterygium wilfordii to lose weight may be dangerous; and the content of tripterygium (charantin) in bitter melon is extremely low, and the function of tripterygium is dose-dependent, thus hindering the development of tripterygium as a weight loss new drug process
However, there is no report of extracting tripterycin from buckwheat plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for extracting tripterygne, which uses golden buckwheat, tartary buckwheat or sweet buckwheat as raw materials for extraction, and the specific extraction steps include the following steps:

[0031] (1) Dry the seeds, leaves, stalks and roots of golden buckwheat, tartary buckwheat or sweet buckwheat, add liquid nitrogen and grind to obtain powder;

[0032](2) Mix the powder with deionized water, adjust the pH value of the solution to 5.0, then add 0.5% powder weight mixed enzyme for enzymolysis, the enzymolysis temperature is 25°C, and the enzymolysis time is 40min. After the enzymolysis is completed, filter , to obtain the filter residue; wherein, the mixed enzyme is a mixture of cellulase and pectinase in a weight ratio of 2:1;

[0033] (3) The filter residue obtained in step (2) is mixed with the extract at a solid-to-liquid ratio of 1g:20mL, and then the ultrasonic power density is 100W / cm 2 1. Ultrasonic extraction is carried out under the condition of ul...

Embodiment 2

[0042] A method for extracting tripterygne, which uses golden buckwheat, tartary buckwheat or sweet buckwheat as raw materials for extraction, and the specific extraction steps include the following steps:

[0043] (1) Dry the seeds, leaves, stalks and roots of golden buckwheat, tartary buckwheat or sweet buckwheat, add liquid nitrogen and grind to obtain powder;

[0044] (2) Mix the powder with deionized water, adjust the pH value of the solution to 6.0, then add 0.8% powder weight mixed enzyme for enzymolysis, the enzymolysis temperature is 50°C, the enzymolysis time is 30min, and filter after the enzymolysis is completed , get filter residue; Wherein, mixed enzyme is the mixture that cellulase and pectinase are mixed in 5:1 by weight ratio;

[0045] (3) The filter residue obtained in step (2) is mixed with the extract at a solid-to-liquid ratio of 1g:80mL, and then the ultrasonic power density is 150W / cm 2 1. Ultrasonic extraction is carried out under the condition of 50kH...

Embodiment 3

[0054] A method for extracting tripterygne, which uses golden buckwheat, tartary buckwheat or sweet buckwheat as raw materials for extraction, and the specific extraction steps include the following steps:

[0055] (1) Dry the seeds, leaves, stalks and roots of golden buckwheat, tartary buckwheat or sweet buckwheat, add liquid nitrogen and grind to obtain powder;

[0056] (2) Mix the powder with deionized water, adjust the pH value of the solution to 6.0, then add 0.6% powder weight mixed enzyme for enzymolysis, the enzymolysis temperature is 35°C, and the enzymolysis time is 40min. After the enzymolysis is completed, filter , to obtain the filter residue; wherein, the mixed enzyme is a mixture of cellulase and pectinase in a weight ratio of 3:1;

[0057] (3) The filter residue obtained in step (2) is mixed with the extract at a solid-to-liquid ratio of 1g:30mL, and then the ultrasonic power density is 120W / cm 2 1. Ultrasonic extraction is carried out under the condition of 3...

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PUM

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Abstract

The invention discloses a method for extracting tripterine. According to the present invention, Fagopyrum plants are used as a raw material, liquid nitrogen grinding, enzymolysis treatment, ultrasonicextraction, extraction, silica gel column separation and recrystallization are sequentially performed, and the finally extracted tripterine has characteristics of high yield and high purity; and themethod of the present invention provides the new raw material and the extraction method for the tripterine extraction process, has the low raw material cost, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant active ingredient extraction, and in particular relates to a method for extracting tripterine. Background technique [0002] Tripterygium wilfordii is as famous as artemisinin, and is listed by "Cell" magazine as one of the five traditional natural medicinal compounds that are most likely to be developed into modern medicines. Tripterygium wilfordii is one of the active ingredients of traditional Chinese medicine Tripterygium wilfordii. In 2015, the research team of Professor Umut Ozcan of Harvard Medical School reported for the first time the weight loss and leptin sensitization effects of Tripterygium wilfordii, which made this traditional Chinese medicine ingredient popular all over the world. In the same year, Elisabetta Mueller from the NIH in the United States also found that tripterygium activating the heat shock factor HSF1 can increase energy consumption and help mice resist high-fat diet-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 刘新宇夏燕莉蔡光泽
Owner 北京浩鼎瑞健康科技中心(有限合伙)
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