High-efficiency and high-sensitivity biomacromolecule trans-membrane and staining system and device

Abstract

The invention discloses a high-efficiency and high-sensitivity biomacromolecule trans-membrane and staining system. The high-efficiency and high-sensitivity biomacromolecule trans-membrane and staining system comprises a working unit and an operating portion which is associated with the working unit, wherein the working unit is provided with at least one channel used for detecting and analyzing a sample, the operating portion is used for creating at least one staining or trans-membrane channel parameter, and each staining or trans-membrane channel parameter comprises at least one electric field cycle. The invention further discloses a device based on the high-efficiency and high-sensitivity biomacromolecule trans-membrane and staining system. The device comprises a base and a shell, an operating panel is arranged on the outside of the shell, a working region, a system power source and a working circuit board are arranged in the base and the shell, the system power source and the working circuit board are connected with the working region, the working region is at least provided with one pull-out-type independent working chamber, and a positive and negative electrode board providing an electric field is installed in the working chamber. According to the system or the device, rapid staining and trans-membrane of biomacromolecules can be controlled by driving movement of biological charged particles through the electric field; meanwhile, the requirement for high sensitivity of biological detection can be further met.

Description

technical field [0001] The invention relates to the technical field of biological detection equipment, in particular to a biomacromolecule transmembrane and dyeing system and equipment that utilizes an electric field to drive the movement of organisms or other charged particles to achieve high efficiency and high sensitivity. Background technique [0002] In a DC electric field, the phenomenon that charged particles move to electrodes with opposite signs is called electrophoresis (electrophoresis). In 1809, the Russian physicist Peнce first discovered the electrophoresis phenomenon, but it was not until Tiselius in Sweden established the boundary electrophoresis (boundary electrophoresis) for separating proteins in 1937 that the electrophoresis technology began to be applied. In the 1960s and 1970s, when media such as filter paper and polyacrylamide gel were successively introduced into electrophoresis, electrophoresis technology developed rapidly. The rich and colorful ele...

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Problems solved by technology

Since the factors affecting the electrophoretic mobility are mainly related to the electric field strength, the pH value of the solution, and the ionic strength of the solution, the mobility of biomacromolecules and dyes in the medium where the electric field is applied is not the same, and biomacromolecules are larger. It moves slowly in the electric field and will not move out of the medium within a limited time. Organic dye molecules are much smaller than biomacromolecules, and dye molecules that are not combined with biomacromolecules can quickly move out of the medium, so they can be In the same electric field, the color development of biomacromolecules can be achieved by moving the dye out of the medium while the biomacromolecule (incorporated with the dye) remains in the medium, so that high resolution, little or no background influence can be obtained in a short time Analytical images of biomacromolecules in the background, but if the dye and biomacromolecules only meet and combine once, it is difficult to meet the requirements for some experiments with relatively high sensitivity requirements, and even prone to missed detection in the detection

[0004] With the rapid development of biomedicine and other disciplines, the detection technology of biomacromolecules is also rapidly improving. However, there is no system or device in the market that can use electric fields to drive the movement of biologically charged particles to control the rapid dyeing and transfection of biomacromolecules. membrane, and at the same time achieve the requirement of high sensitivity

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