Method for activating granulocyte

Abstract

The invention provides a method for activating granulate bodies, which mainly comprises the following steps of: supplying negative charges and active oxygen to the granulate bodies from the outside through a negative charge and active oxygen supply unit so as to serve as raw materials for redox of the granulate bodies; the calcium ion channel promoting unit promotes the calcium ions in the body to enter the matrix space of the granulonema through calcium transport proteins on the granulonema inner membrane, so that the internal flow of the calcium ions can promote the depolarization of the granulonema, and the oxidative phosphorylation activity of the granulonema is improved. Thus, granulocyte activation and ATP production are enhanced.

Description

TECHNICAL FIELD

[0001] The present application relates to a method for activating mitochondria, in particular, providing negative charges and reactive oxygen species to mitochondria in vitro through a negative charge and reactive oxygen species supply unit, and promoting the influx of calcium ions into the matrix space of mitochondria through a calcium ion channel promoting unit to enhance the activation of mitochondria and ATP production. BACKGROUND

[0002] Mitochondria are the units of energy production in cells, which are the energy factories of cells. Each mitochondrion is composed of an outer membrane and an inner membrane, and the space enclosed by the inner membrane is called the matrix space. The outer membrane of mitochondria is embedded with enzymes related to biological oxidation reactions, which provide the raw materials required for the internal reactions of mitochondria. In the interior of mitochondria, the matrix space contains enzymes that can metabolize carbohydrates, proteins, and fats into H2O and CO2 through the citric acid cycle. In this process, electrons are transferred through the respiratory enzyme chain, and through oxidative phosphorylation, high-energy phosphate compounds, adenosine triphosphate (ATP), are synthesized. ATP is the main energy source for energy-consuming reactions in plants and animals. It is a nucleotide in biochemistry that serves as the "energy currency" of cellular energy transfer, storing and transferring chemical energy. It also plays an important role in nucleic acid synthesis. It is also a guanine dinucleotide in RNA sequences, which can be used as a substitute when DNA is transcribed.

[0003] However, mitochondria need to be constantly oxidized and reduced to produce energy. The starting power of oxidation and reduction depends on negative charges (-e) and reactive oxygen species (O), which are the raw materials for mitochondria to function. If the above-mentioned raw materials of mitochondria are provided, mitochondria can be more activated. In addition, the influx of calcium ions plays an important role in the activation of mitochondria and ATP production:

[0004] 1. Regulation of calcium ions in mitochondria: Calcium ions can enter mitochondria and affect their function. When the concentration of calcium in the cytoplasm increases, calcium ions enter the matrix space of mitochondria through calcium transport proteins on the inner membrane of mitochondria. This influx of calcium ions can promote the depolarization of mitochondria, thereby increasing the oxidative phosphorylation activity of mitochondria.

[0005] 2. Promoting oxidative phosphorylation: The increase in calcium ions activates various enzyme systems within the mitochondria, such as the key enzymes in the tricarboxylic acid cycle (TCA). The activation of these enzymes enables the mitochondria to speed up the oxidation process of substrates and increase the efficiency of the electron transport chain, ultimately increasing ATP production.

[0006] 3. Increasing ATP synthesis: When the calcium concentration within the mitochondria increases, the rate of the tricarboxylic acid cycle (TCA cycle) and oxidative phosphorylation increases. Oxidative phosphorylation is a metabolic pathway in cells that occurs on the inner mitochondrial membrane in eukaryotes or the cell membrane in prokaryotes, using enzymes and the energy released from the oxidation of various nutrients to synthesize adenosine triphosphate (ATP), which directly increases ATP production. ATP production is driven by the operation of the electron transport chain, and the calcium ion-promoted processes ensure the sustainability and efficiency of ATP production.

[0007] 4. Maintaining cellular energy balance: Increased ATP production helps cells maintain their energy balance, especially when responding to various stresses. This also helps cells repair and restore normal function, avoiding cell death due to energy deficiency.

[0008] Therefore, if the mitochondria are constantly supplied with the negative charges (-e) and reactive oxygen species (O) required for mitochondrial redox, and the calcium ions in the body are promoted to enter the matrix space of the mitochondria through the calcium transport protein on the inner membrane of the mitochondria, the activation of the mitochondria and the production of ATP can be enhanced. SUMMARY

[0009] The main purpose of the present application is to provide a method for activating mitochondria that can enhance mitochondrial activation and ATP production.

[0010] The main feature of the present application is that in step 1, the mitochondria are supplied with negative charges and reactive oxygen species from the outside by a negative charge and reactive oxygen species supply unit as raw materials for mitochondrial redox; in step 2, the calcium ions in the body are promoted to enter the matrix space of the mitochondria through the calcium transport protein on the inner membrane of the mitochondria by a calcium ion channel promotion unit, so that the inward flow of calcium ions can promote the depolarization of the mitochondria and improve the oxidative phosphorylation activity of the mitochondria. Thus, the activation of the mitochondria and the production of ATP are enhanced.

[0011] In the above method for activating mitochondria, the negative charge and reactive oxygen species supply unit is a low-temperature plasma generator.

[0012] In the method for activating mitochondria, the calcium channel promoting unit is a far infrared ray generator with a power of 500 watts or more. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 A block diagram of a preferred embodiment of the present application is shown.

[0014] Reference numerals in the drawings are explained as follows:

[0015] Negative charge and active oxygen supply unit 1 Calcium channel promoting unit 2 DETAILED DESCRIPTION

[0016] Referring to Figure 1 A method for activating mitochondria according to a preferred embodiment of the present application is shown, and the method is as follows:

[0017] Step 1: Providing negative charge (-e) and active oxygen (O) to mitochondria; the negative charge and the active oxygen are provided to mitochondria from outside by a negative charge and active oxygen supply unit 1, which serves as a raw material for oxidation and reduction of mitochondria; the negative charge and active oxygen supply unit 1 can be implemented by energizing a low-temperature plasma generator. When the low-temperature plasma generator is energized, a large amount of negative charge (-e) and active oxygen (O) is generated, which enters the body as a raw material for oxidation and reduction of mitochondria.

[0018] Step 2: Providing calcium ions to mitochondria; the calcium channel promoting unit 2 promotes calcium ions in the body to enter the matrix space of mitochondria through calcium transport proteins on the inner membrane of mitochondria, so as to promote calcium ion influx and depolarization of mitochondria. The calcium channel promoting unit 2 can be a far infrared ray generator with a power of 500 watts or more, which is used to irradiate the body to promote calcium ions in the body to enter the matrix space of mitochondria through calcium transport proteins on the inner membrane of mitochondria.

[0019] Through the negative charge and active oxygen supply unit 1 in step 1, sufficient negative charge (-e) and active oxygen (O) raw materials are provided to mitochondria, and through the calcium channel promoting unit 2 in step 2, calcium ions in the body are promoted to enter the matrix space of mitochondria through calcium transport proteins on the inner membrane of mitochondria, so as to promote calcium ion influx and depolarization of mitochondria, thereby enhancing activation of mitochondria and ATP production.

[0020] Secondly, the present application detects the mitochondrion activation of peripheral blood mononuclear cells of subjects A, B and C before using the present application, one hour after using the present application for the second time and one hour after using the present application for the fourth time, respectively. The detection results are shown in the first, second and third times of the first page of the annex. The detection results show that the mitochondrion is obviously activated after using the present application, which proves that the present application has good efficacy and practicality.

Claims

1. A method for activating mitochondria, characterized in that, include: Step 1: Provide negative charge and reactive oxygen species to the mitochondria; The negative charge and active oxygen are supplied to the mitochondria from the outside through the negative charge and active oxygen supply unit, so as to serve as raw materials for the oxidation and reduction of the mitochondria; Step 2: Provide calcium ions to the mitochondria; promote calcium ions in the body through the calcium ion channel promoting unit to enter the matrix space of the mitochondria via calcium transport proteins on the inner membrane of the mitochondria, so that the influx of calcium ions can promote mitochondrial depolarization and improve the oxidative phosphorylation activity of the mitochondria.

2. The method for activating mitochondria according to claim 1, characterized in that: The negative charge and active oxygen supply unit is a cryogenic plasma generator.

3. The method for activating mitochondria according to claim 1 or 2, characterized in that: The calcium ion channel promoting unit is a far-infrared generator with a power of 500 watts or more.

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