Biocolloid hemostatic prepared by aldehyde-modified sodium alginate and amine-modified gelatine

A sodium alginate and aldehyde modification technology, applied in medical science, absorbent pads, bandages, etc., can solve problems such as systemic or local biological reactions, and achieve the effect of convenient preparation methods

Inactive Publication Date: 2010-06-02
TIANJIN UNIV
View PDF0 Cites 68 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Iwata et al. reported that water-soluble carbodiimide-crosslinked PLGA-gelatin bioglue and PLGA-gelatin bioglue modified by N-hydroxysuccinimide can rapidly form a gel when the two materials are mixed together And it is firmly bonded to soft tissue. However, due to the release of urea derivatives or N-hydroxysuccinimide during the formation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Weigh 5.0 g of sodium alginate and disperse it in 25 ml of absolute ethanol.

[0022] (2) Weigh 1.35g of sodium periodate and dissolve it in 25ml of deionized water, and keep away from light during the whole process.

[0023] (3) The solution (2) was mixed with the solution (1), and reacted at 25° C. in the dark for 6 hours, and then 5 ml of ethylene glycol was added to terminate the reaction for 15 minutes.

[0024] (4) Put the solution (3) into a dialysis bag for dialysis for 3 days and freeze-dry it? °C to obtain aldehyde-modified sodium alginate.

[0025] (5) Weigh 2g of gelatin and dissolve it in 50ml of 0.1M phosphate buffer solution (pH 5.0), add 5.6g of ethylenediamine, and adjust the pH to 5.0 with 6N hydrochloric acid solution.

[0026] (6) Add 1.07g water-soluble carbodiimide (iodide [1-cyclohexyl-3-(3-trimethylaminopropyl) carbodiimide]) to solution (5), then add phosphate buffer solution Total volume up to 100ml. React at 37°C for 1 hour.

[0027] (7...

Embodiment 2

[0032] Raw material is substantially identical with embodiment 1. (2) in embodiment 1 is adjusted to sodium periodate addition and is 2.7g, and other steps are identical. Its pigskin bonding strength is 150gfcm -1 , The gel time is 80 seconds.

Embodiment 3

[0034] Raw material is substantially identical with embodiment 2. (3) in Example 2 was adjusted to react in the dark at 4°C, and the other steps were the same. Its pigskin bonding strength is 182gfcm -1 , The gel time was 62 seconds.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a biocolloid hemostatic prepared by aldehyde-modified sodium alginate and amine-modified gelatine. Sodium alginate and gelatine as raw materials; according to ratio, aldehyde-modified sodium alginate solution with the concentration of 5-25% and the same volume of amine-modified gelatine solution with the concentration of 5-40% are mixed; sodium alginate is modified by aldehyde through sodium periodate oxidation; and then gel is obtained by mixing with gelatine modified by ethanediamine. The adopted gelatine and sodium alginate are safe and stable, have good biocompatibility and can be absorbed and degraded by organism. Gel is rapidly formed through Schiff base reaction of aldehyde-modified sodium alginate and amine-modified gelatine, and the gel has the same gel-forming time as fibrin glue but better adhesion property and adhesion strength, can be acted on any part of body through simple local spraying or injection, and can be used as substitute product of fibrin glue.

Description

technical field [0001] The present invention relates to a kind of biological glue type hemostatic agent of aldehyde-modified sodium alginate and amine-modified gelatin, specifically, it is the biological glue-type soft tissue hemostatic agent which is composed of aldehyde-modified sodium alginate and amine-modified gelatin, through The chemical reaction of the groups contained in the two materials quickly gels and stops the bleeding. Background technique [0002] With the rapid development of modern science and technology, surgical operation techniques have undergone revolutionary changes. Hemostasis is one of the cores of basic surgical techniques, and surgical operations on any part of the human body almost without exception involve bleeding and hemostasis. In the past, the traditional instrumental hemostasis technology has been abandoned due to the disadvantages of complicated operation and large trauma. Therefore, it is expected and paid attention to the emergence of a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L15/32A61L15/28A61L15/62
Inventor 尹玉姬朴明伟孙言蓓
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap