Novel bio-medical adhesive and preparation method thereof

A biomedical and adhesive technology, used in pharmaceutical formulations, surgical adhesives, applications, etc., can solve the problems of insufficient adhesion strength, high adhesion strength, and low biocompatibility in the humid environment in the body, and achieve water permeability and breathability. Good property, low swelling rate, good effect

Active Publication Date: 2016-04-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a biomedical adhesive capable of in-situ cross-linking with the wound and its preparation and use method in view of the low biocompatibility of the existing adhesive and the insufficient adhesive strength in the wet environment of the body. The adhesive has good biocompatibility, can be completely degraded and absorbed by the body, has high bonding strength under wet conditions in the body, and has the functions of filling, sealing and hemostasis, and can promote tissue healing and repair at the same time, and can be used as a medical tissue Adhesives, sealants or hemostats for clinical wound repair

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of oxidized sodium alginate: Weigh 10g of sodium alginate, disperse it in 25mL of absolute ethanol, then weigh 2g of sodium periodate and dissolve it in 25mL of distilled water in the dark. Stir the reaction with magnetic force in the dark for 2 hours, add 10 mL of ethylene glycol to terminate the reaction for 0.5 hours, and finally dialyze with distilled water for 1 day, centrifuge the retentate at 10,000 rpm for 10 minutes to remove the precipitate, and put the supernatant under the conditions of pressure 0 Pa and temperature -100 °C Under freeze-drying for 48 hours, oxidized sodium alginate is obtained;

[0034](2) Preparation of oxidized sodium alginate solution grafted with dopamine: Weigh a certain amount of oxidized sodium alginate, dissolve it in MES buffer solution with a pH of 4.5, and prepare a 2% oxidized sodium alginate solution; Under the protection of nitrogen, a certain mass ratio of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochlo...

Embodiment 2

[0038] (1) Preparation of oxidized sodium alginate: Weigh 10g of sodium alginate, disperse it in 50mL of absolute ethanol, then take 4g of sodium periodate in the dark and dissolve it in 50mL of distilled water. Stir the reaction for 6 hours in the dark, add 20 mL of ethylene glycol to terminate the reaction for 1 hour, and finally dialyze with distilled water for 2 days, centrifuge the retentate at 15,000 rpm for 20 minutes to remove the precipitate, and put the supernatant under the conditions of pressure 10 Pa and temperature -50 °C Freeze-dry for 60 hours to obtain oxidized sodium alginate;

[0039] (2) Preparation of dopamine-grafted oxidized sodium alginate solution: Weigh a certain amount of oxidized sodium alginate, dissolve it in MES buffer solution with a pH of 5.5, and make a oxidized sodium alginate solution with a mass concentration of 6%; Under the protection of nitrogen, a certain mass ratio of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) an...

Embodiment 3

[0043] (1) Preparation of oxidized sodium alginate: Weigh 10g of sodium alginate, disperse it in 100mL of absolute ethanol, then weigh 6g of sodium periodate and dissolve it in 100mL of distilled water in the dark. Stir the reaction in the dark for 10 hours, add 30 mL of ethylene glycol to terminate the reaction for 2 hours, and finally dialyze with distilled water for 3 days, centrifuge the retentate at 20,000 rpm for 30 minutes to remove the precipitate, and put the supernatant under the conditions of pressure 20 Pa and temperature -30 °C Freeze-dry for 72 hours to obtain oxidized sodium alginate;

[0044] (2) Preparation of dopamine-grafted oxidized sodium alginate solution: Weigh a certain amount of oxidized sodium alginate, dissolve it in MES buffer solution with a pH of 6.5, and prepare a 10% oxidized sodium alginate solution; Under the protection of nitrogen, a certain mass ratio of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimi...

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Abstract

The invention discloses a bio-medical adhesive, which can be cross-linked with a wound in situ, and a preparation method of the bio-medical adhesive. The preparation method is characterized by comprising the steps of first, preparing oxidized sodium alginate by using sodium periodate to oxidize sodium alginate; dissolving the oxidized sodium alginate in an MES (Methyl Ester Sulfonate) buffer solution, dissolving 1-(3-dimethyl aminopropyl)-3-ethyl carbodiimide hydrochloride and N-hydroxyl succinimide in the MES buffer solution as well under nitrogen protection, continuously adding dopamine, carrying out dialyzing and freeze-drying after reacting for 8 to 24 hours, and obtaining A liquid by dissolving a reaction product in a PBS (Phosphate Buffer Solution) or a sodium borate solution; then, dissolving I-shaped collagen in an acid solution, and obtaining B liquid by neutralizing the solution pH (potential of Hydrogen) to be 5 to 8; firstly pre-treating the wound by using hydrogen peroxide or HRP (Horse Radish Peroxidase) before the bio-medical adhesive is used, then immediately coating the wound with the bio-medical adhesive after mixing the A liquid with the B liquid, and forming gel in 30 to 120 s, wherein the gel is firmly adhered to the surface of the wound. The bio-medical adhesive disclosed by the invention has a stronger adhesive force under a moist environment in vivo, integrates filling, sealing and bleeding stopping functions, is good in biocompatibility, is bio-degradable and can be used for promoting wound healing.

Description

technical field [0001] The invention relates to a medical wound adhesive with good biocompatibility, biodegradability and strong adhesive force, and functions of filling, sealing and hemostasis, and a preparation and use method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] Over the years, the most commonly used method of suturing wounds clinically is to use sutures, rivets and other materials or instruments to suture. This method ensures the tensile strength, but it will increase the pain of the patient, leave scars after the wound is healed, and the operation is troublesome. With the rapid development of modern medicine, the clinical requirements for wound bonding are getting higher and higher, not only to minimize the patient's pain and shorten the healing time, but also to restore the appearance perfectly while restoring the function. Tissue adhesives are widely used clinically because of their short bonding time, si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/00A61L24/04A61L26/00
CPCA61L24/0031A61L24/0042A61L24/043A61L26/0052A61L26/008A61L26/009A61L2400/04C08L5/04
Inventor 但卫华崔国廉但年华刘新华
Owner SICHUAN UNIV
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