Method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin

A technology of hyaluronic acid and cross-linked gelatin, applied in application, pharmaceutical formulation, surgery, etc., can solve problems such as cross-linking agent poisoning or adverse reactions, and achieve good mechanical properties, good biocompatibility, and improved hemostatic properties. Effect

Active Publication Date: 2016-03-16
稳得希林(沈阳)生物科技有限公司
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Problems solved by technology

These small molecule cross-linking agents are prone to poisoning or adverse reactions

Method used

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  • Method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin

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Embodiment 1

[0018] 1) Dissolve 5g of hyaluronic acid (molecular weight: 1.5 million) in 100mL of deionized water, dissolve 1g of sodium periodate in 50mL of deionized water; slowly add the sodium periodate solution dropwise into the hyaluronic acid solution, and react 10h; after the reaction, dialyze with a dialysis bag with a molecular weight cut-off of 3500 for 24h; finally freeze-dry to obtain the formaldehyde hyaluronic acid cross-linking agent;

[0019] 2) Weigh 3g gelatin and dissolve it in 50mL water, stir and dissolve at 50°C; then add 3g glycerin; weigh 0.2g formaldehyde hyaluronic acid cross-linking agent and dissolve it in 5mL deionized water; weigh 1g poloxamer 407 to dissolve Add 25 mL of phosphate buffer solution with a pH value of 8, stir until completely dissolved; add the hydroformylated hyaluronic acid cross-linking agent solution to the gelatin solution, and stir evenly; add the poloxamer 407 solution to the above solution, Continue to stir at 40°C for 30 minutes;

[0...

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Abstract

The invention discloses a method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin and belongs to the field of biomedical materials. The method includes the steps that firstly, a novel hydroformylation hyaluronic acid crosslinking agent is prepared; then, the hydroformylation hyaluronic acid crosslinking agent is mixed with gelatin for foaming, refrigerating and drying are conducted, and the gelatin hemostatic cotton can be obtained after sterilization. According to the method, strong oxidant sodium periodate is utilized to oxidize a carbon-carbon bond of cis diol in the hyaluronic acid so that the hyaluronic acid can react with amidogen for formylation; the hyaluronic acid obtained after formylation serves as a macromolecular crosslinking agent and reacts with the gelatin containing an amino functional group, and the gelatin is made to form a cross-linked structure, so that mechanical performance is enhanced, and the sponge function is given to the gelatin. The gelatin is good in biocompatibility, has the hemostatic and anti-microbial functions and serves as a sponge base material, the hydroformylation hyaluronic acid serves as the crosslinking agent, the prepared hemostatic cotton is good in mechanical performance, safe and non-toxic, the hemostatic property is remarkably improved, a good pain relieving function is achieved, and the effect of promoting healing of injured tissues is achieved.

Description

technical field [0001] The invention relates to a method for preparing hemostatic cotton by using hyaluronic acid cross-linked gelatin, which belongs to the field of biomedical materials. Background technique [0002] Currently commonly used gelatin sponge has a single function and low hemostatic efficiency. In order to further improve the strength and hemostatic performance of gelatin hemostatic cotton. In the present invention, the gelatin hemostatic cotton is improved by using the hydroformylated hyaluronic acid as the cross-linking agent. Traditional cross-linking agents commonly used today include glutaraldehyde and formaldehyde. In the Chinese invention patent CN103877606A, a composite sponge was prepared by using small molecule genipin and glutaraldehyde as cross-linking agents; Small molecules such as ether were used as cross-linking agents to prepare composite hemostatic cotton. These small molecule cross-linking agents are prone to poisoning or adverse reaction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L5/08C08K5/053C08J9/28C08J3/24C08B37/08A61L24/08A61L24/10
CPCA61L24/0015A61L24/0036A61L24/08A61L24/104A61L2300/252A61L2300/404A61L2400/04C08B37/0072C08J3/246C08J9/28C08J2201/0484C08J2389/00C08J2405/08C08L89/00C08L2203/02C08L2312/00C08L5/08C08K5/053
Inventor 徐福建胡浩罗旭
Owner 稳得希林(沈阳)生物科技有限公司
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