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Biological medical oxidative sodium carboxymethylcellulose-dopamine adhesive and preparation method thereof

Sodium carboxymethyl cellulose, biomedical technology, applied in the field of medical wound adhesives, can solve the problems of insufficient adhesive strength and low biocompatibility, and achieve the effects of simple production, good effect and low cost

Inactive Publication Date: 2016-07-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a biomedical adhesive capable of in-situ cross-linking with the wound and its preparation and use method in view of the low biocompatibility of the existing adhesives and the insufficient adhesive strength in the wet environment of the body , the medical adhesive has good biocompatibility, the degradation process is controllable, and can be completely degraded and absorbed by the body. Clinical wound repair as a medical tissue adhesive, sealant or hemostat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of oxidized sodium carboxymethylcellulose: Weigh 2g of sodium carboxymethylcellulose and dissolve it in 50mL of acetic acid-sodium acetate buffer solution with a pH of 2.0, stir until clear at room temperature, then add 0.5g of high Sodium iodate was dissolved in 25 mL of the above buffer solution in the dark, and slowly added to the above sodium carboxymethyl cellulose solution, and the temperature was 25 °C under the condition of dark stirring reaction for 24 hours, after the reaction was completed, 5 mL of ethylene glycol was added, and continued Stir for 0.5h, then add 2g of sodium chloride, and slowly add 200ml of methanol, stand still, filter with suction, put the precipitated precipitate into a dialysis bag with a molecular weight cut-off of 3000~3500Da, dialyze with distilled water for 1 day, and take the retentate Freeze-dry for 48 hours at a pressure of 5Pa and a temperature of -100°C to obtain sodium oxidized carboxymethylcellulose;

[0022] (...

Embodiment 2

[0024](1) Preparation of oxidized sodium carboxymethyl cellulose: Weigh 2 g of sodium carboxymethyl cellulose and dissolve it in 100 mL of acetic acid-sodium acetate buffer solution with a pH of 3.5, stir until clear at room temperature, then add 1 g of periodine Dissolve sodium carboxymethyl cellulose in 50 mL of the above buffer solution in the dark, and slowly add it to the above sodium carboxymethyl cellulose solution, and stir for 36 hours at a temperature of 35°C in the dark, and add 10 mL of ethylene glycol after the reaction is complete, and continue stirring 0.5h, then add 3g of sodium chloride, and slowly add 300ml of methanol, stand still, filter with suction, put the separated precipitate into a dialysis bag with a molecular weight cut-off of 8000~12000Da, dialyze with distilled water for 2 days, take the retentate in Freeze-drying at a pressure of 15Pa and a temperature of -60°C for 60 hours to obtain bisaldehyde carmellose sodium;

[0025] (2) Preparation of oxid...

Embodiment 3

[0027] (1) Preparation of oxidized sodium carboxymethylcellulose: Weigh 2g of sodium carboxymethylcellulose and dissolve it in 200mL of acetic acid-sodium acetate buffer solution with a pH of 5.0, stir at room temperature until clear, and then add 2g of periodine Dissolve sodium carboxymethyl cellulose in 100 mL of the above buffer solution in the dark, and slowly add it to the above sodium carboxymethyl cellulose solution, stir and react for 48 hours at a temperature of 40°C in the dark, add 20 mL of ethylene glycol after the reaction is complete, and continue stirring 1h, then add 5g of sodium chloride, and slowly add 400ml of methanol, stand still, filter with suction, put the separated precipitate into a dialysis bag with a molecular weight cut-off of 3000~3500Da, dialyze with distilled water for 3 days, take the retentate under pressure Freeze-drying at 20Pa and temperature -30℃ for 72h to obtain bisaldehyde carmellose sodium;

[0028] (2) Preparation of oxidized carboxym...

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Abstract

The invention discloses a biological medical adhesive capable of producing in situ crosslinking with a wound and a preparation method of the adhesive.The adhesive is prepared with the following method: firstly, sodium periodate oxidative sodium carboxymethylcellulose-dopamine is used for preparing dialdehyde sodium carboxymethylcellulose, a product is dissolved in an MES buffering solution after dialysis and drying, then a certain amount of 1(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-H-hydroxysuccinimide are dissolved in the MES buffering solution as well, stirring reaction is performed by 10-40 min, dopamine is added then, stirring reaction is performed by 8-24h under nitrogen protection, and dialysis, lyophilization and vacuum preservation are performed.Before using, hydrogen peroxide or horse radish peroxidase is used for pretreating the wound, then the wound is coated with the lyophilized adhesive after the lyophilized adhesive is dissolved in a PBS buffering solution, and the wound can be bonded within 2-10 min.The biological adhesive has higher bonding strength under a moist environment in a body, the biocompatibility is good, and the adhesive is biodegradable and capable of promoting wound healing.

Description

technical field [0001] The invention relates to a medical wound adhesive with good biocompatibility, biodegradability and strong adhesive force, and functions of filling, sealing and hemostasis and a preparation method thereof, belonging to the field of preparation of biomedical materials. Background technique [0002] In recent years, with the development of science and technology, the medical level has gradually improved, and the clinical requirements for surgical operations and auxiliary materials have also become higher and higher. However, traditionally, sutures, rivets and other materials or instruments are used for wound bonding. This method is not only cumbersome to operate, but repeated dressing changes and stitch removal will increase the pain of the patient, leaving scars on the healed wound and even interfering with the recovery of tissue function, which will undoubtedly cause secondary harm to the patient. Therefore, people have been eager to develop an ideal ti...

Claims

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Application Information

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IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/08A61L24/0042A61L2300/232A61L2300/412A61L2300/602A61L2300/604A61L2400/04C08L1/286
Inventor 但卫华崔国廉但年华
Owner SICHUAN UNIV
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