Method for preparing bone grafting material with pilose antler and deer bone as material and the bone grafting material prepared by the method

A technology of deer antler and deer bone is applied in the field of preparing bone graft materials, which can solve the problems of adverse effects of clinical application, cross infection, fear of patients, etc., and achieve the effects of good bone conduction, reasonable price and abundant resources.

Inactive Publication Date: 2010-01-06
王喜文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Bone transplantation is an important means of orthopedic repair surgery. Research on bone banks and bone graft materials has always been an important topic in orthopedics. Bone banks at home and abroad mainly store allogeneic bone, but due to the limited source of allogeneic bone, there are potential risks of hepatitis B and AIDS. The risk of cross-infection cannot meet the various needs of clinical bone graft materials. In recent years, there have been recombined heterogeneous bone materials in China. This product is a composite of BMP extracted from bovine cortical bone and bovine cancellous bone carrier without antigen Through a series of animal experiments and clinical studies, it has been proved that it not only induces osteogenesis and good bone conduction, but also has good biocompatibility. It is an applicable bone graft material. Due to the mad cow disease in Europe, the The clinical application of domestic recombined heterogeneous bone products has brought adverse effects, and some patients have a psychological fear of bone graft materials extracted from bovine bones that can promote osteogenesis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take the two bars of deer antler as raw material, weigh 100 grams, and use a grinder to powder into 100 mesh fine powder. Take out half and add 200ml of 0.1Mole / L sodium carbonate buffer solution with a pH of 8.5-8.8. After stirring for 3 hours at room temperature, add 100ml of acetone and continue stirring for 2 hours, and filter to obtain the extract for use. Add the other half of velvet antler powder to 200ml of 0.001 / L hydrochloric acid solution, pH 3.5-4.0, stir at room temperature for 3 hours, add 100ml of acetone and continue stirring for 2 hours, filter the extract and mix with the obtained extract, and then extract the mixture Place the liquid in a freezer for storage, add 2500ml of cold acetone, and dehydrate it twice to obtain a precipitate. The precipitate is dissolved in 250ml of non-pyrogenic water and ultrafiltered by an ultrafilter with a retention of 70,000 daltons. The molecular weight is less than 70,000 daltons. The part with a cut-off of 50,000 Dalto...

Embodiment 2

[0041] Take red antler lotus as raw material, weigh 100 grams, and use a grinder to powder into 100 mesh fine powder. Take out half and add 200ml of 0.1Mole / L sodium carbonate buffer solution, adjust the pH to 8.5, stir at room temperature for 3 hours, add 100ml of acetone and continue to stir for 2 hours, and filter to obtain the extract for use. Add the other half of the deer antler powder to 200ml of 0.001Mole / L hydrochloric acid solution, adjust the pH to 4, stir at room temperature for 3 hours, add 100ml of acetone and continue stirring for 2 hours, filter the extract and mix with the obtained extract, and then extract the mixture Place the liquid in a freezer for storage, add 2500ml of cold acetone, and dehydrate the precipitate twice. Dissolve the precipitate with 250ml of sterile water. After ultrafiltration with a 70,000 dalton ultrafilter, the molecular weight is less than 70,000 daltons. The part with a cut-off of 50,000 Daltons is ultrafiltered, and the part with a ...

Embodiment 3

[0045] Take the Sancha of deer antler as raw material, weigh 100 grams, and use a grinder to powder into 100 mesh fine powder. Take out half and add 200ml of 0.1Mole / L sodium carbonate buffer solution with a pH of 8.5-8.8. After stirring for 3 hours at room temperature, add 100ml of acetone and continue stirring for 2 hours, and filter to obtain the extract for use. Add the other half of the deer antler powder to 200ml of 0.001Mole / L hydrochloric acid solution, adjust the pH to 3.5-4, stir at room temperature for 3 hours, add 100ml of acetone and continue to stir for 2 hours, filter the extract and mix with the obtained extract, then mix the The mixed extract is placed in a freezer for storage, and 2500ml of cold acetone is added, and the precipitate is dehydrated in two times. The precipitate is dissolved in 250ml of sterile water, and ultrafiltered by an ultrafilter with 70,000 daltons. The molecular weight is less than 70,000. The part with a molecular weight of 50,000 dalto...

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PUM

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Abstract

The present invention discloses a method for preparing bone grafting material by using velvet deerhorn and deer's bone as raw material and bone grafting material prepared by adopting said preparation method. It is characterized by that said invention utilizes the extract of velvet deerhorn and deer's cancellous bone to prepare bone-grafting material, its resource is rich and its application is safe, and said bone-grafting material contains several growth factors and velvet deerhorn polypeptide, so that it has obvious bone induction action, and can obtain extensive application.

Description

Technical field [0001] The invention relates to a method for preparing a bone graft material, in particular a method for preparing a bone graft material using deer antler and deer bone as raw materials and a bone graft material prepared by the method. Background technique [0002] There are many breeding farms in Jilin, Liaoning, Heilongjiang, Hebei, Beijing and other places to raise sika deer and red deer. The medical and pharmaceutical value of sika deer and red deer has attracted more and more attention. The first is antler, which is the young horn of sika deer. More with 1 to 2 branches. Those with 1 branch are usually called "two bars", and those with 2 branches are called "three forks". The second stubble is similar to the first stubble. Red deer antler is also known as green fur. It is the young horn of red deer, the shape is thicker than that of flower antler, and there are more branches. One side branch is usually called "single door", two are called "lotus", three a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
Inventor 李德山王喜文
Owner 王喜文
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