ZIF-8 drug-loaded hydrogel osteogenesis-promoting scaffold, preparation method and application

A ZIF-8, bone-promoting technology, used in pharmaceutical formulations, drug delivery, pharmaceutical science, etc., can solve problems such as insufficient promotion of bone regeneration, secondary damage to donors, lack of personalized design, etc., to improve bone tissue biochemistry. The effect of microenvironment, reducing adipogenesis, good application prospect

Active Publication Date: 2021-11-23
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Through the above analysis, bone defects, especially patients with hyperlipidemia, are still a difficult problem in clinical treatment. In recent years, bone tissue engineering hydrogel scaffolds are often used in bone defect repair, but there are still some problems and defects: with the improvement of people's living standards Lipid metabolism disorders represented by hyperlipidemia are increasing year by year, and are often accompanied by bone metabolic diseases such as osteoporosis; Susceptible to infection, bone nonunion, etc.
However, its disadvantage is that the source of bone mass is limited, which will cause secondary damage to the donor, cause immune rejection of the body, and increase the risk of infectious diseases and complications.
However, the existing bone tissue engineering hydrogel materials still have the following defects: 1) the mechanical strength is not ideal, and the mechanical microenvironment for bone cell growth is poor; 2) there is no personalized design for the repair of irregular bone defects; 3) Insufficient osteoinductivity, unable to fully promote bone regeneration; 4) For the repair of bone defects with systemic diseases (such as hyperlipidemia, diabetes, etc.), there is a lack of efficient osteogenic materials for targeted treatment

Method used

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  • ZIF-8 drug-loaded hydrogel osteogenesis-promoting scaffold, preparation method and application
  • ZIF-8 drug-loaded hydrogel osteogenesis-promoting scaffold, preparation method and application
  • ZIF-8 drug-loaded hydrogel osteogenesis-promoting scaffold, preparation method and application

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preparation example Construction

[0052] Such as figure 1 As shown, the novel ZIF-8 drug-loaded hydrogel provided by the embodiments of the present invention promotes the preparation method of bone scaffold comprising:

[0053] S101, construction of SIM@ZIF-8 drug-loading system;

[0054] S102, Construction of SIM@ZIF-8 / PEGDA / SA hydrogel.

[0055] Such as figure 2 As shown, the construction of the SIM@ZIF-8 drug-carrying system provided by the embodiments of the present invention includes:

[0056] S201, dissolving 3.25g of dimethylimidazole in 100ml of methanol solvent, vigorously stirring at room temperature to form a transparent and uniform solution;

[0057] S202, 1.47g of Zn(NO 3 ) 2 ·6H 2 O and 0.15g of SIM were dissolved in 50ml of methanol solution, stirred for 30min after ultrasonic treatment for 5min;

[0058] S203, adding dimethylimidazole solution dropwise to the solution obtained in S202, stirring while adding, fully stirring for 2 hours;

[0059] S204, after the reaction is completed, the ...

Embodiment 1

[0068] 1. In order to further improve the osteoinductivity and lipid-lowering properties of the material, and give full play to the advantages of ZIF-8 drug loading and sustained release, the present invention loads SIM in the pore structure of ZIF-8 by in situ synthesis (SIM@ZIF-8 ), improving the biochemical microenvironment of bone tissue to achieve the dual effect of reducing fat and forming osteogenesis.

[0069] The invention utilizes the photoinitiation performance and mechanical properties of PEGDA, the viscoelasticity of SA, and constructs SIM@ZIF-8 / PEGDA / SA three-dimensional composite injectable photocurable bioactive slow-release scaffold by adding SIM@ZIF-8 drug-loading system, To improve the biochemical microenvironment of bone cell growth. In the bioactive SIM@ZIF-8 / PEGDA / SA hydrogel system, PEGDA and SA cross-linked to form the first network, PEGDA photo-cross-linked to form the second network, Zn in ZIF-8 crystals 2+ The cross-linked sodium alginate hydrogel i...

Embodiment 2

[0090] In order to prove the effect of this material on bone formation and fat reduction, the present invention uses synthetic ZIF-8 / PEGDA / SA and PEGDA / SA as control samples.

[0091] 1. The synthesis method of ZIF-8 / PEGDA / SA hydrogel is as follows:

[0092] The synthesis of described ZIF-8 powder comprises:

[0093] (1) Dissolve dimethylimidazole in 100ml of methanol solvent, and vigorously stir at room temperature to form a transparent and uniform solution;

[0094] (2) Zn(NO 3 ) 2 ·6H 2 O was dissolved in 50ml of methanol solution, sonicated for 5min and then stirred for 30min;

[0095] (3) Add step (1) dimethylimidazole solution dropwise to the solution obtained in step (2), stir while adding, and fully stir for 2 hours;

[0096] (4) After the reaction finishes, the resulting solution is centrifuged at 10000 rpm / min for 10 min, and washed three times repeatedly with 10 ml of methanol;

[0097] (5) The washed material was vacuum-dried at 37° C. for 48 h to finally obt...

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Abstract

The invention belongs to the technical field of metal-organic frameworks, and discloses a ZIF-8 drug-loaded hydrogel osteogenesis-promoting scaffold, a preparation method and application, and the preparation method of the ZIF-8 drug-loaded hydrogel osteogenesis-promoting scaffold comprises the steps of construction of an SIM-ZIF-8 drug-loaded system and construction of the SIM-ZIF-8 / PEGDA / SA hydrogel. The novel ZIF-8 drug-loaded hydrogel osteogenesis-promoting scaffold is SIM-ZIF-8 / PEGDA / SA. According to the invention, an in-situ synthesis method is adopted to load SIM in a ZIF-8 pore structure (SIM-ZIF-8), and the biochemical microenvironment of bone tissues is improved, so that the dual effects of lowering lipid and forming bones are achieved. According to the invention, the ZIF-8 is loaded with an SIM small molecule drug for the first time, and the finally formed SIM-ZIF-8 system can reduce fat formation and promote osteogenesis under a high fat condition, and has a good application prospect in treatment of bone metabolic diseases caused by hyperlipidemia.

Description

technical field [0001] The invention belongs to the technical field of metal-organic frameworks, and in particular relates to a ZIF-8 drug-loaded hydrogel to promote bone scaffolding, a preparation method and an application. Background technique [0002] At present, metal-organic frameworks (MOFs) are a new class of crystalline materials with metal ions as coordination centers, which have extremely high specific surface area, high porosity, structural diversity and unsaturated metal Coordination bonds are beneficial for modifying or grafting other molecular substances. In recent years, they have been more and more widely used in the field of biomedicine, especially in the field of drug delivery. ZIF-8 is a class of MOF materials with osteoinductive ability. It has good stability, structure-function adjustability, osteoinductivity and drug-loading potential. It has gradually attracted attention in tissue engineering. The results showed that titanium nanoscale ZIF-8 coating c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/52A61L27/56A61L27/20A61L27/02A61L27/18
CPCA61L27/54A61L27/52A61L27/56A61L27/20A61L27/025A61L27/18A61L2430/02A61L2300/602A61L2300/40A61L2400/06A61L2300/102A61L2300/412C08L5/04C08L71/02
Inventor 朱舟乔铭薪黄炜万乾炳王剑裴锡波陈俊宇
Owner SICHUAN UNIV
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