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Injected bone repairing material and its prepn and application

A bone repair and injection-type technology, which is used in bone diseases, medical preparations containing active ingredients, medical science, etc., can solve problems such as difficult shaping operations, complex exogenous cytokines, etc. Healing, evenly distributed effect

Inactive Publication Date: 2006-10-18
NAN FANG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned existing technical conditions, in order to overcome the shortcomings of existing bone repair materials such as difficulty in shaping, complicated operation, and expensive exogenous cytokines, the present invention provides an injectable bone repair material, which is an injectable bone repair material. Shaped material loaded with autologous PRP and then compounded with autologous bone marrow stromal cells, which can be shaped arbitrarily and the composite cells are simple, and autologous cell growth factors are used, so there is no immune rejection problem

Method used

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  • Injected bone repairing material and its prepn and application
  • Injected bone repairing material and its prepn and application
  • Injected bone repairing material and its prepn and application

Examples

Experimental program
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Embodiment 1

[0036] Example 1: Seed cells—cultivation and induction of differentiation of bone marrow stromal stem cells.

[0037] The seed cells used in this experiment are bone marrow stromal stem cells, which are used together with the injection-type bone repair material of the present invention to construct tissue engineered bone.

[0038] New Zealand white rabbits, provided by the Animal Institute of Nanfang Hospital, Southern Medical University (formerly First Military Medical University), were anesthetized by intramuscular injection of Sumianxin, ketamine and atropine compound solution. Extract 3ml of red bone marrow from bilateral iliac crests, anticoagulate with heparin solution, mix into DMEM medium, centrifuge at 1000r / min for 10min after mixing, discard the suspended fat cells and part of the supernatant, pass through a 100-mesh filter, and add DMEM complete medium (containing 15% fetal bovine serum) inoculation. 37°C, 5% CO 2 Culture in an incubator, change the full amount o...

Embodiment 2

[0039] Embodiment two: the extraction of PRP.

[0040] Under aseptic conditions, 5ml of central artery blood was extracted from the dorsal ear of rabbits, and anticoagulated with 0.5ml of 10% sodium citrate. Centrifuge at 3000r / min for the first time at 20°C for 10 minutes, and absorb the supernatant and 3 mm components below the buffy coat. Centrifuge for the second time at the same temperature at 3600r / min for 15 minutes, discard the upper 3 / 4 supernatant, and the remaining part is PRP. Vortex gently to mix. The platelet content was counted under a high power microscope and compared with whole blood. The number of platelets in PRP prepared by this method is more than 4 times higher than that in whole blood. Make about 0.6ml of PRP per 5ml of whole blood, and store it in a -70°C refrigerator for later use.

Embodiment 3

[0041] Example 3: Preparation of injection-type bone repair materials.

[0042] The injection type bone repair material consists of solution A and solution B. Solution A is to dissolve fibrinogen with aprotinin solution and mix it with PRP at a ratio of 3-6:1. The concentration of aprotinin is 2000~6000U / ml. Solution B is calcium chloride and thrombin solution, the concentration of calcium chloride is 40mmol / ml, and the concentration of thrombin is 400U / ml. When in use, draw solution A and solution B separately with a double syringe. Mix and inject solutions A and B at a ratio of 9:1 to 1:1. It forms a jelly-like gel within 20-30 seconds when injected into the body, and also forms a gel in vitro.

[0043] When used to construct tissue engineered bone, before solution A and B are mixed and injected, bone marrow stromal stem cells induced to differentiate and proliferate in vitro according to the method described in Example 1 are added to solution A, and the cell density is ...

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Abstract

The present invention discloses one kind of injected bone repairing material and its preparation and application. The injected bone repairing material consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg / ml concentration fibrinogen solution in the ratio of 1 to 3-6; and the solution II is solution mixture of calcium chloride in 40 mmol / ml concentration and thrombin in 400-1000 U / ml concentration. When the injected bone repairing material is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected bone repairing material is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

Description

technical field [0001] The invention belongs to the technical field of preparing artificial organs by tissue engineering methods in biomedical engineering, and in particular relates to an injection type bone repair material and its preparation and application. Background technique [0002] At present, most bone repair materials are scaffold materials loaded with cell growth factors. The selection of scaffold materials and growth factors is the focus of tissue engineering research. Scaffold materials, as seed cell carriers, have the function of loading cells and slowing the release of growth factors. At present, most commonly used scaffold materials are solid materials, which have achieved remarkable results in the preparation of bone repair materials. However, there are also defects such as difficulty in shaping, complex operation of loading growth factors and seed cells, low loading rate, and easy loss of cells to varying degrees. Therefore, injectable or hydrogel materia...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K47/42A61L27/22A61P19/08
Inventor 裴国献黄爱文金丹曾宪利胡稷杰陈书军张元平
Owner NAN FANG HOSPITAL
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