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Humanization active forging bone and preparation method thereof

A calcined bone and humanized technology, applied in medical science, prosthesis, etc., can solve the problems of difficult immunogenicity, difficult combination, and limited application, achieving non-carcinogenic and teratogenic effects, convenient clinical operation, The effect of moderate degradation rate

Active Publication Date: 2009-10-28
SHAANXI RUISHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent uses a collagen coating, which is difficult to form a firm bond with the material; and after calcining bone composite cells, it is difficult to apply clinically due to the immunogenicity of the cells; at the same time, it is difficult to prefabricate irregular bone defects into the desired shape , these deficiencies limit the practical application of this material

Method used

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  • Humanization active forging bone and preparation method thereof
  • Humanization active forging bone and preparation method thereof
  • Humanization active forging bone and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Removal of organic matter: take bovine femoral epiphyseal cancellous bone, remove the soft tissue on the surface, machine it into broken bone pieces with a particle size of 5mm, stir and wash them with pure water for 30 minutes; put the broken bone pieces at a concentration of 0.5g / L trypsin solution was digested at room temperature for 24 hours, stirred and washed with pure water, then ultrasonically cleaned in 1M NaOH solution for 2 hours, the solution was changed every half hour, stirred and cleaned with pure water until neutral; 2 o 2 The solution was treated at room temperature for 40 minutes, stirred and washed with pure water, and dried in a 37°C oven until dry.

[0023] 2. Calcination: Put the dried bone fragments into a corundum crucible and calcinate in a muffle furnace; raise the temperature to 800°C, continue calcination at a constant temperature for 4 hours, lower the temperature in the furnace to room temperature, and take out the calcined bone;

[0024...

Embodiment 2

[0030] 1. Remove organic matter: Take the cancellous bone of the bovine vertebrae to remove the soft tissue on the surface, machine it into broken bone pieces with a particle size of 10mm, stir and wash them with pure water for 90 minutes; put the broken bone pieces into 1.5g / L trypsin solution at room temperature Digested for 48 hours, thoroughly stirred and cleaned with pure water, then ultrasonically cleaned in 1M NaOH solution for 3 hours, the solution was replaced every 1 hour, fully stirred and cleaned with pure water until neutral; 2 o 2 The solution was treated for 50 minutes, thoroughly stirred and washed with pure water, and dried in a 37°C oven until dry.

[0031] 2. Calcination: put the dried bone fragments into a corundum crucible, and calcinate in a muffle furnace; raise the temperature to 600°C, continue to calcine at a constant temperature for 3 hours, lower the temperature in the furnace to room temperature, and then raise the temperature to 1000°C, and keep t...

Embodiment 3

[0038] 1. Removal of organic matter: Take porcine femoral epiphyseal cancellous bone to remove the soft tissue on the surface, process it into broken bone pieces with a particle size of 20mm, stir and wash with pure water for 60 minutes; add 2g / L trypsin solution to digest the broken bone pieces at room temperature for 72 hours , after stirring and cleaning with pure water, put it in 1M NaOH solution and ultrasonically clean it for 4 hours, replace the solution every 1 hour, and ultrasonically clean it with pure water until it is neutral; 2 o 2 The solution was treated for 60 minutes, stirred and washed with pure water, and dried in a 37°C oven until dry.

[0039] 2. Calcination: put the dried bone fragments into a corundum crucible, and calcinate in a muffle furnace; heat up to 700°C, and calcine at a constant temperature for 4 hours, then lower the temperature in the furnace to room temperature, and then heat up to 1100°C, and calcine at a constant temperature After 3 hours...

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Abstract

The invention discloses a humanization active forging bone and a preparation method thereof. The humanization active forging bone has main components of hydroxyapatite crystal, natural animal cancellous bones, particles of which the particle size is between 0.1 and 5 millimeters and having three-dimensional netlike porous structures, and a coating of which the pore-size distribution is between 50 and 600 microns and the surface is compounded with extracellular matrix synthesized and excreted by human cells. The forging bone not only has a similar structure with a human bone and is advantageous for cell ingrowth, formation of new bones, and bone structure reconstruction at a transplantation position, but also can be firmly combined with the coating of which the surface is coated with the extracellular matrix synthesized and excreted by the human cells and cell growth factors, promotes the attached growth of in vivo osteoblasts, and accelerates the formation of the new bones; and simultaneously, the forging bone has the advantages of molding randomly and not causing immunological rejection reactions, and can satisfy treatment needs of clinical bone defect and nonunion.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biomaterials, and in particular relates to a humanized active calcined bone and a preparation method thereof. Background technique [0002] Bone transplantation is one of the main methods for treating bone defects. Usually autologous bone transplantation has the best effect, but due to the limited source of autologous bone, it will increase the patient's pain and surgical complications. However, artificially synthesized bone substitute materials (such as bioceramics, calcium phosphate bone cement, polymer polymers, and metal materials, etc.) have more complicated manufacturing processes, higher requirements for the production process, and may contain harmful factors that endanger the health of patients. Some are difficult to absorb and replace after being implanted in the body, hindering the reconstruction and repair of bone tissue. An ideal bone graft material should have an internal ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/56A61L27/54A61L27/58A61L27/40A61L27/12
Inventor 王爱军
Owner SHAANXI RUISHENG BIOTECH
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