Culture method for composition of periosteal biological scaffold and allogenic seed cells

A technology for biological scaffolds and seed cells, which is applied in the field of bioengineering and can solve problems such as the destruction of extracellular matrix components and structures, the difficulty of cell recombination, and the impact on the biocompatibility of scaffolds.
CN104511052AInactive Publication Date: 2015-04-15THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
Publication Date
2015-04-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a culture method for the composition of a periosteal biological scaffold and allogenic seed cells. The culture method comprises the following steps: the non-immunogenic periosteal biological scaffold and the seed periosteal cells are prepared; the non-immunogenic periosteal biological scaffold is sealed after low-temperature drying; after gamma-ray disinfection of the non-immunogenic periosteal biological scaffold, the seed periosteal cells, with the concentration of 1*106 per milliliter, are dripped on the surface of the disinfected scaffold; a culture medium is added after the periosteal cells gradually permeate into the non-immunogenic periosteal biological scaffold together with a suspension, the medium change is carried out every 2 to 3 days, and the in-vitro composite culture lasts for one week. The periosteal biological scaffold obtained through the method has the characteristics of complete removal of immunogenic cells, as well as good preservation of the structure and major components of extracellular matrix; effective composition of the allogenic periosteal cells and the biological scaffold can be achieved, so that a biological composite scaffold material can be provided for tissue engineering researches on bone defects and bone nonunion.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a compound culture method of periosteum biological scaffold and allogeneic seed cells. Background technique

[0002] The periosteum has a strong bone-forming ability and plays an important role in fracture healing and repair. The periosteum is also considered to be the initiating factor that promotes the repairing effect of bone graft materials in the grafted area. However, autologous periosteum transplantation has problems such as insufficient donors and damage to the structure and function of the donor site, while allogeneic periosteum transplantation has serious problems such as immune rejection. Therefore, solving the immune rejection problem in allogeneic periosteum will be an important link in realizing periosteum transplantation. The main immunogenic components in tissues are cells, and the removal of cells also means the removal of immunogenicity. The...

Claims

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