Dual-network hydrogel composition, dual-network hydrogel biological scaffold, preparation method of scaffold, and application of composition

A bio-scaffold and hydrogel technology, applied in prosthesis, medical science, etc., can solve problems such as lack of functionality, achieve excellent biological safety, maintain long-term structural stability, and excellent structural fidelity

Pending Publication Date: 2020-03-27
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing single-component hydrogel bioinks lack functionality in the system culture process, such as the contradiction between the printability of the ma

Method used

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  • Dual-network hydrogel composition, dual-network hydrogel biological scaffold, preparation method of scaffold, and application of composition
  • Dual-network hydrogel composition, dual-network hydrogel biological scaffold, preparation method of scaffold, and application of composition
  • Dual-network hydrogel composition, dual-network hydrogel biological scaffold, preparation method of scaffold, and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A double-network hydrogel composition, comprising formylated hyaluronic acid, carboxymethyl chitosan, vinyl gelatin and photocrosslinking initiator, wherein, the form of formylated hyaluronic acid and carboxymethyl chitosan The mass ratio is 1:1, the mass ratio of vinyl gelatin to carboxymethyl chitosan is 1:1, and the photocrosslinking initiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methanol Propiophenone.

[0051] A dual-network hydrogel bioscaffold, such as figure 1 As shown, the biological scaffold includes a first network structure and a second network structure, wherein the first network structure is an interpenetrating network structure formed by the Michael addition reaction of formylated hyaluronic acid and carboxymethyl chitosan, and the first The second network structure is an independent network structure that runs through the first network structure formed by overlapping vinyl gelatin photo-induced free radical polymerization in the grid combined by the firs...

Embodiment 2

[0066] A double network hydrogel bioscaffold, said bioscaffold includes the first network formed by the Michael addition reaction of aldylated hyaluronic acid and carboxymethyl chitosan and the photoinitiated radical polymerization reaction of vinyl gelatin. The second network is prepared from the double network hydrogel composition of Example 1,

[0067] The preparation steps are as follows:

[0068] S1 Prepare an aldylated hyaluronic acid (A-HA) solution with a concentration of 50 mg / mL, and the solvent is a cell culture medium solution with endothelial cells (concentration is about 1,000,000 cells / ml);

[0069] S2 Dissolve vinyl gelatin (GelMA) in carboxymethyl chitosan (CM-CTS) solution to obtain a mixed solution of vinyl gelatin and carboxymethyl chitosan solution, the concentrations of CM-CTS and GelMA are both 50mg / mL;

[0070] S3 Add a photocrosslinking initiator to the mixed solution of vinyl gelatin and carboxymethyl chitosan solution. The mass of photoinitiator I...

Embodiment 3

[0080] A double-network hydrogel composition, comprising formylated hyaluronic acid, carboxymethyl chitosan, vinyl gelatin and a photocrosslinking initiator, wherein the formylated hyaluronic acid, carboxymethyl chitosan, The mass ratio of vinyl gelatin is 1:1:4, and the photocrosslinking initiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone.

[0081] A double-network hydrogel bioscaffold, said bioscaffold includes a first network formed by the Michael addition reaction of aldylated hyaluronic acid and carboxymethyl chitosan and a photoinitiated radical polymerization reaction of vinyl gelatin. The second network is prepared from the above-mentioned double network hydrogel composition,

[0082] The preparation steps are as follows:

[0083] S1 Prepare an aldylated hyaluronic acid (A-HA) solution with a concentration of 100mg / mL, and the solvent is water;

[0084] S2. Vinyl gelatin (GelMA) was dissolved in carboxymethyl chitosan (CM-CTS) solution to obtain a mixed...

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Abstract

The invention discloses a dual-network hydrogel composition, a dual-network hydrogel biological scaffold, a preparation method of the scaffold, and an application of the composition. The double-network hydrogel composition comprises aldehyde hyaluronic acid, carboxymethyl chitosan, vinyl gelatin and a photo-crosslinking initiator, wherein a mass ratio of the aldehyde hyaluronic acid to the carboxymethyl chitosan is 1:1 to 5:1, and a mass ratio of the vinyl gelatin to the carboxymethyl chitosan is 1:2 to 5:1. The aldehyde hyaluronic acid and carboxymethyl chitosan in the composition are reactedto obtain a first network, the vinyl gelatin photo-initiates free radical polymerization to prepare a second network structure, the formed double-network hydrogel biological scaffold has good structural stability, good cell compatibility, good biological safety and excellent structural fidelity, and the compression modulus of the hydrogel biological scaffold can be adjusted to 10-100 KPa by controlling the proportions of all the components, so the hydrogel biological scaffold is suitable for printing various tissue bodies, and the activity of printed cells is 80% or above, thereby the application of the cell biological scaffold material is further expanded.

Description

technical field [0001] The present invention relates to the technical field of biomedical materials, more specifically, to a double network hydrogel composition, a double network hydrogel bioscaffold, and a preparation method and application thereof. Background technique [0002] Today, materials such as hyaluronic acid, chitosan, and gelatin have been extensively studied as degradable scaffolds in the field of tissue engineering. Hyaluronic acid (HA) is a biomedical material that is naturally degradable and its degradation products can be absorbed by the body. It is also widely used in clinical practice. HA has strong viscoelasticity and lubricity, and can form a continuous macromolecular network structure after cross-linking, which can not only maintain good biocompatibility and natural biodegradation metabolism, but also have better mechanical strength and stability. sex. High-molecular-weight hyaluronic acid can actively stimulate the proliferation and migration of end...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08L89/00C08J3/075A61L27/52A61L27/20A61L27/22A61L27/50
CPCC08J3/075A61L27/52A61L27/20A61L27/222A61L27/50C08J2305/08C08J2489/00
Inventor 张馨之马骋邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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