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Preparation method of polyester material capable of preventing platelet adhesion without affecting platelet function

A polyester material, anti-platelet technology, applied in anti-coagulation treatment, packaging item types, special packaging items, etc., can solve the problem of increased leukocyte removal during filtration, the influence of platelet function has not been paid attention to, and platelet adhesion and platelet function. Affect unresearched problems, and achieve the effect of improving blood compatibility, improving hydrophilicity and blood compatibility, and inhibiting platelet activation

Active Publication Date: 2016-09-21
BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of the study showed that the recovery rate of platelets could be significantly improved after phosphatidylcholine group modified polyester non-woven fabrics, but the effect on platelet function has not been paid attention to
The latest research uses ligands such as polypeptides that are coated or grafted on the surface of the material to specifically bind to leukocyte receptors. While reducing the filtration time, leukocyte removal is also significantly increased, but the effect on platelet adhesion and platelet function has not been studied.

Method used

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  • Preparation method of polyester material capable of preventing platelet adhesion without affecting platelet function
  • Preparation method of polyester material capable of preventing platelet adhesion without affecting platelet function
  • Preparation method of polyester material capable of preventing platelet adhesion without affecting platelet function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation method of polyester material that resists platelet adhesion and does not affect platelet function:

[0035] S1. Soak the polyester material in 3 mg / mL polyethyleneimine solution for 1 hour, and carry out amination treatment on the material;

[0036] S2. Soak the polyester material in a dopamine solution (prepared in PBS, pH=7.4, concentration 2mg / mL), add hydrogen peroxide (wt5%-15%), and shake at a constant temperature for 12 hours to make dopamine self-polymerize on the surface of the polyester material to form polydopamine coating;

[0037] S3. Soak the aminated polyester material obtained in S1 or the dopamine-coated polyester material obtained in S2 in sodium heparin solution (prepared in acetate buffer solution, pH=4.0, concentration 1 mg / mL), shake at constant temperature for 15 minutes, Loading heparin on the surface of the material through electrostatic interaction, and then rinsing with acetate buffer solution with pH=4.0 for 2-4 times;...

Embodiment 2

[0040] Example 2: Changing the reaction conditions to construct a hydrophilic polyester material through layer-by-layer electrostatic self-assembly

[0041] S1. Soak the polyester material in a dopamine solution (prepared in PBS, pH=7.4, concentration 1mg / mL), add hydrogen peroxide (wt5%-15%), and shake at a constant temperature for 6 hours to make dopamine self-polymerize on the surface of the polyester material to form polydopamine coating;

[0042] S2. Soak the dopamine-coated polyester material obtained in S1 in sodium heparin solution (prepared in acetate buffer solution, pH=4.0, concentration 1 mg / mL), shake at constant temperature for 15 minutes, so that heparin can be loaded on the surface of the material through electrostatic action , and then rinsed 2-4 times with acetate buffer solution of pH=4.0;

[0043] S3. Soak the polyester material of surface self-assembled polyanionate obtained in S2 in a solution of dopamine (prepared in PBS, pH=7.4, concentration 2mg / mL), ...

Embodiment 3

[0045] Example 3: Regulating the hydrophilicity and surface heparin content of polyester materials through the number of assembled layers

[0046] The layer-by-layer electrostatic self-assembled polyester material was prepared according to the method of Example 1 and Example 2, and the hydrophilicity of the polyester material assembled with different layers and different polyelectrolytes was characterized by static water contact angle. The toluidine blue method was used to detect the heparin content on the surface of the material, and the specific method was as follows:

[0047] S1 draw standard curve

[0048] Prepare 10, 20, 30, 40, 50, 60, 70, and 80 μg / mL heparin standard solutions, and accurately pipette 0.25 mL of 0.005% toluidine blue solution (prepared with 0.01 mol HCl solution containing 0.2% NaCl) to 8 Into a clean test tube, add 0.1mL of heparin standard solution of different concentrations, then add 1mL of 0.2% NaCl solution, immediately vortex vigorously, then im...

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Abstract

The invention discloses a preparation method of a polyester material capable of preventing platelet adhesion without affecting a platelet function and belongs to the field of surface modification of biological materials. According to the method, firstly, with the adoption of ammonolysis, the surface of the polyester material has positively charged amino or dopamine forms a positively charged dopamine coating on the surface of the material through auto polymerization, the polyester material with positive ions is immersed into a negatively charged biomacromolecule solution, and the processes are performed repeatedly until hydrophilic and biocompatible biomacromolecules are fixed on the surface of the polyester material through layer-by-layer electrostatic self-assembly. By means of the method, static water contact angles of the polyester material can be effectively reduced, the hydrophile of the material is improved, platelet adhesion can be further prevented through introduction of biomacromolecules, and no remarkable influence is caused to gathering and other functions of platelet.

Description

technical field [0001] The invention belongs to the technical field of surface modification of biological materials, in particular to a preparation method of a polyester material that resists platelet adhesion and does not affect platelet function. Background technique [0002] Polyester materials, especially non-woven fabrics, have been widely used in medical and health fields, especially white blood cell filter materials, due to their large specific surface area and porosity, good physical and mechanical properties and easy processing. However, polyester materials have few polar functional groups on the surface, strong hydrophobicity, and poor blood compatibility, which limit their application in the field of biomedical materials. Therefore, hydrophilic modification must be carried out. Common modification methods include ultraviolet grafting, High-energy radiation, plasma modification, immersion coating, etc. [0003] Polyester materials need to be in direct contact with...

Claims

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Application Information

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IPC IPC(8): C08J7/12A61L33/10A61L33/08A61L33/14A61L33/06C08L67/00
Inventor 钟锐刘嘉馨王红贺曾韩玎玎张学俊
Owner BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI
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