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ZnO quantum dot vector/DNA composite-containing collagen-based composite cornea substitute, and preparation method and application thereof

A substitute and quantum dot technology, applied in the field of collagen-based composite corneal substitutes, can solve the problems of biotoxicity restricting applications, achieve easy processing, long-term storage and transportation, improve stability and mechanical strength, and promote corneal regeneration. Effect

Inactive Publication Date: 2012-12-12
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, CdX (X=S, Se, Te) quantum dots are the most widely used and most maturely prepared, but their great biological toxicity seriously restricts their application in the field of gene therapy.

Method used

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  • ZnO quantum dot vector/DNA composite-containing collagen-based composite cornea substitute, and preparation method and application thereof
  • ZnO quantum dot vector/DNA composite-containing collagen-based composite cornea substitute, and preparation method and application thereof
  • ZnO quantum dot vector/DNA composite-containing collagen-based composite cornea substitute, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The proportion of each component of the collagen corneal substitute is as follows:

[0033] Pig skin type Ⅰ collagen: MPDSAH (mass ratio) 1:0

[0034] Coll-NH 2 :EDC:NHS (molar ratio) 1:1:1

[0035] (1) Preparation of cross-linked collagen corneal substitute: Take 0.5g of 13.7% pigskin type Ⅰ collagen solution into a syringe, and connect another syringe to seal through a T-shaped container, push and pull repeatedly to mix well, and then use a micro-syringe to measure Inject crosslinkers EDC and NHS, where Coll-NH 2 : EDC: NHS = 1: 1: 1 (molar ratio), push and pull repeatedly to mix evenly, then add an appropriate amount of 2mol / L NaOH solution with a micro syringe to adjust the pH to about 5.5. After mixing evenly, the solution was poured into the mold, and then put into the clamp to fix it, 100% humidity, reacted at room temperature for 16 hours, and then moved into an oven at 37°C for aging for 5 hours. Remove the jig, open the mold and take out the corneal sample...

Embodiment 2

[0039] The proportions of the components of the collagen / MPDSAH composite corneal substitute are as follows:

[0040] Pig skin type Ⅰ collagen: MPDSAH (mass ratio) 1:0.3

[0041] Coll-NH 2 :EDC:NHS (molar ratio) 1:1:1

[0042] MPDSAH: PEGDA (mass ratio) 2:1

[0043] Irgacure2959: MPDSAH (molar ratio) 0.02:1

[0044] The steps of preparing the bioactive composite corneal substitute with the above-mentioned components are as follows:

[0045] (1) Preparation of composite corneal substitute: Take 0.5g of 13.7% pigskin type Ⅰ collagen solution into a syringe, and connect another syringe to seal through a T-shaped container, push and pull to mix evenly; the measured amount is pig skin type Ⅰ collagen : The MPDSAH of MPDSAH=1:0.3 (mass ratio) is dissolved in water, moves into the T-shaped container through the sealing pad with a micro-injector, pushes and pulls repeatedly to mix evenly, then injects cross-linking agent EDC, NHS and photoinitiator Irgacure 2959 sequentially with ...

Embodiment 3

[0049] The proportions of the components of the collagen / MPDSAH composite corneal substitute are as follows:

[0050] Pig skin type Ⅰ collagen: MPDSAH (mass ratio) 1:1

[0051] Coll-NH 2 :EDC:NHS (molar ratio) 1:1:1

[0052] MPDSAH: PEGDA (mass ratio) 2:1

[0053] Irgacure2959: MPDSAH (molar ratio) 0.02:1

[0054] The steps of preparing the bioactive composite corneal substitute with the above-mentioned components are as follows:

[0055] (1) Preparation of composite corneal substitute: Take 0.5g of 13.7% pigskin type Ⅰ collagen solution into a syringe, and connect another syringe to seal through a T-shaped container, push and pull to mix evenly; the measured amount is pig skin type Ⅰ collagen : The MPDSAH of MPDSAH=1:1 (mass ratio) is dissolved in water, moves into the T-shaped container through the sealing gasket with a micro-injector, pushes and pulls repeatedly to mix evenly, then injects cross-linking agent EDC, NHS and photoinitiator Irgacure 2959 sequentially with a...

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Abstract

This invention relates to a method of fabricating collagen-based hydrogels loaded with ZnO QDs / pDNA complexes as corneal substitutes. Polycation-modified ZnO Quantum Dots were encapsulated into IPN hydrogels by the adsorption effect of freeze-dried hydrogels. The weight ratio of substitutes and ZnO QDs complex is approximately 425:1. And the weight ratio of ZnO QDs / pDNA is 25:1. This kind of corneal substitutes possess favorable biocompatibility. It is able to induce and promote the regeneration of the cornea and it will degrade along with the regeneration of the cornea. The incorporation of the MPDSAH can enhance the stability of corneal substitutes under the existence of collagenase. ZnO QDs used in this invention can condense DNA effectively and ferry DNA into cells successfully. In the process of transfection, the location and distribution of DNA / vector can be tracked by fluorescence in real time. What's more, the convenience of preparation, long term storage and transportation offers a general method to fabricate a promising corneal substitute.

Description

technical field [0001] The invention relates to a collagen-based composite corneal substitute containing a ZnO quantum dot carrier / DNA complex and a preparation method and application thereof. It is a collagen-based composite corneal substitute loaded with a bioimaging function transgene carrier and a preparation method thereof, and uses tissue engineering and gene transfection to treat ophthalmology-related diseases. Background technique [0002] As a blinding eye disease with high incidence, corneal disease has been paid more and more attention by people. Since the endothelial cells of the cornea cannot be repaired or replaced automatically, allogeneic keratoplasty has become the most effective method of treating corneal diseases, but allogeneic keratoplasty has resulted in a great demand for donor corneas, and there is an unhealthy In addition, the incidence of postoperative immune rejection is still high. In view of this, it is urgent to develop an alternative to alloge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/16A61L27/54
CPCA61L2300/624A61L27/24A61K9/19A61L27/54A61K9/0051A61L27/52A61K31/711A61K33/08A61L2400/12A61L2430/16A61K9/0012A61K33/30A61L2300/258A61P27/02A61K2300/00
Inventor 刘文广刘贵培杨建海张鹏
Owner TIANJIN UNIV
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