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Method for constructing anti-blood coagulation blood vessel scaffold by utilizing endogenous NO donor

A vascular stent and anticoagulant technology, which is applied in the field of constructing anticoagulant vascular stent materials by using endogenous NO donors, can solve the problems of affecting the persistence of NO release, fast release rate, and limited application, and achieve inhibition of smooth muscle cells. Proliferation, prevention of vascular restenosis, wide selection of effects

Active Publication Date: 2012-11-28
江苏万成生物医学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of NO-releasing polymer materials is a new way to improve the anticoagulant properties of artificial blood vessels, due to the limited amount of NO that can be stored and released by such NO-releasing polymer materials, the release rate is too fast, so Affects the persistence of NO release, which largely limits its application in long-term implant materials, which is the biggest limitation of using this type of material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Utilize endogenous NO donor to construct the method for anticoagulant artificial vascular stent material, comprise the following steps in turn:

[0018] ① Preparation of polyethylenimine (SePEI) with organic selenium immobilized:

[0019] Dipropionyl diselenide (SeDPA) was first activated with EDC and NHS. Then mix the mixture with PEI(M w 25kD) in MES buffer (pH 6.0) for 2 hours. The product SePEI was separated by centrifugation. The Se content in SePEI polymer was quantified by ICP-MS.

[0020] ②L-b-L self-assembly method forms a multilayer bimolecular film on the surface of a porous vascular stent material formed by electrospinning polycaprolactone (PCL):

[0021] First, SePEI and polyglutamic acid (PGA) were formulated into 1 mg / ml solutions in PBS (pH 7.4), and polydiallyldimethylammonium chloride (PDDA) was dissolved in biological buffer CHES (2 -Cyclohexylaminoethanesulfonic acid) (pH 9.3) to make a 1 mg / ml solution. The surface of the PCL po...

Embodiment 2

[0022] Embodiment 2 The method for constructing an anticoagulant metal vascular stent material by using an endogenous NO donor comprises the following steps in sequence:

[0023] ①Preparation of polylysine (SePLL) loaded with organic selenium:

[0024] Dipropionyl diselenide (SeDPA) was first activated with EDC and NHS. Then mix the mixture with polylysine (PLL) (M w 25kD) in MES buffer (pH 6.0) for 2 hours. The product SePLL was isolated by centrifugation. The Se content in the SePLL polymer was quantified by ICP-MS.

[0025] ②L-b-L self-assembly method forms a multi-layer bimolecular film on the surface of the metal vascular stent material:

[0026] SePLL and polyalginic acid (ALG, M w 30kD) were formulated into a 1mg / ml solution in PBS (pH 7.4), and polydiallyldimethylammonium chloride (PDDA) was dissolved in CHES (pH 9.3) to prepare a 1mg / ml solution. The surface of the finished metal vascular stent material is first coated with PDDA and ALG for 10 minutes each to ...

Embodiment 3

[0027] Example 3 Method for Constructing Anticoagulant Co-spun Fiber Porous Artificial Vascular Stent Material Using Endogenous NO Donor

[0028] Co-spun fiber (PHA MCL / PLA) The inner surface of the porous artificial vascular stent material will be organic selenized polycation polymer——water-soluble chitosan (SeCS, M w 30kD) and polyanionic high polymer - polymalic acid (PMA, M w 10kD) is alternately coated by a layer-by-layer self-assembly method to form a multilayer bimolecular film of water-soluble chitosan / polymalic acid (SeCS / PMA); the number of L-b-L layers is 12 layers.

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Abstract

The invention discloses a method for constructing an anti-blood coagulation blood vessel scaffold material by utilizing an endogenous NO donor. The method comprises the steps that organic selenium polycation high polymer and polyanion high polymer are alternately coated on the surface of the blood vessel scaffold material in sequence through a layer-by-layer self-assembled method to form a blood vessel scaffold material surface decorated by a multilayer bimolecular film, thus catalyzing the endogenous NO carries such as S-nitroso glutathione (GSNO) existing in the body, realizing sustained and partial release of NO, effectively regulating and controlling the release rate of NO by regulating and controlling the number of layers of the layer-by-layer self-assembled bimolecular film, and further improving blood coagulation resistance of the blood vessel scaffold material. The method of the invention can be used for metal blood vessel scaffolds or organic polymer artificial blood vessel scaffolds or cell-free artificial blood vessel scaffolds, and also can be used for other medical appliances contacting with blood.

Description

Technical field: [0001] The invention relates to a method for modifying the surface of a tissue engineering vascular stent material, in particular to a method for constructing an anticoagulant vascular stent material by using an endogenous NO donor. Background technique: [0002] Arteriosclerotic heart disease and its corresponding peripheral vascular disease are the diseases with the highest morbidity and mortality at present, which lead to narrowing of arterial diameter and eventually blockage of blood vessels. At present, the terminal treatment methods for this vascular disease mainly include vascular dilation surgery and vascular transplantation using vascular stents. Both vascular stent expansion and vascular grafting have a high failure rate, mainly manifested as thrombosis, vascular stenosis, and blockage. Therefore, how to improve the biocompatibility and biological function of vascular stents and tissue engineering vascular materials, develop safer and more effecti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/10A61L33/02
Inventor 王淑芳孔德领张磊孔梅梅陈思原洪彦航李岩冬宋存江
Owner 江苏万成生物医学研究院
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