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Preparation method for three-dimensional ordered stephanoporate chitosan bone renovating bracket material

A technology of porous chitosan and three-dimensional order, which is applied in the field of preparation of scaffold materials for bone repair, can solve the problems of uneven pore size distribution, low strength, disordered structure, etc., and achieve high strength and porosity, good regularity, The effect of easy operation

Inactive Publication Date: 2009-02-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the past, methods such as secondary freeze-drying and particle filtration were used to prepare porous chitosan scaffold materials. The obtained materials often have uneven pore size distribution, disordered structure, and low strength, which in turn affects the growth and diffusion of cells inside the scaffold, which is not conducive to tissue repair

Method used

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  • Preparation method for three-dimensional ordered stephanoporate chitosan bone renovating bracket material
  • Preparation method for three-dimensional ordered stephanoporate chitosan bone renovating bracket material
  • Preparation method for three-dimensional ordered stephanoporate chitosan bone renovating bracket material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) take by weighing 20g chitosan powder and be dissolved in 500ml volume fraction and be 2% dilute acetic acid, make mass fraction be the chitosan solution of 4%, leave standstill for defoaming;

[0020] 2) uniformly coating the chitosan solution in step 1) on the surface of the mold, placing the mold in a NaOH coagulation solution with a mass fraction of 5%, coagulating the chitosan into a film at room temperature, and demoulding to obtain a chitosan film template;

[0021] 3) Pour the chitosan solution prepared in step 1) into the chitosan membrane template, seal the seal, and immerse in a 5% NaOH coagulation solution with a mass fraction, solidify for 4h, and demould to obtain a chitosan gel, Its cross-section is as figure 1 As shown in , the ordered structure of concentric circles can be seen, and the longitudinal section is as follows figure 2 As shown, the layered superposition order structure can be seen;

[0022] 4) Wash the chitosan gel with distilled water...

Embodiment 2

[0027] 1) taking by weighing 25g chitosan powder is dissolved in the dilute acetic acid that volume fraction is 2%, makes the chitosan solution that mass fraction is 5%, leaves standstill defoaming;

[0028] 2) uniformly coating the chitosan solution in step 1) on the surface of the mold, placing the mold in a NaOH coagulation solution with a mass fraction of 5%, coagulating the chitosan into a film at room temperature, and demoulding to obtain a chitosan film template;

[0029] 3) Pour the chitosan solution prepared in step 1) into the chitosan film template, seal it, and immerse it in a 5% NaOH coagulation solution with a mass fraction, solidify for 8 hours, and demould to obtain a chitosan gel;

[0030] 4) The chitosan gel was washed with distilled water until neutral, dried in an oven at 70°C for 90 minutes, and then freeze-dried in a freeze dryer at -70°C for 60 hours to obtain a three-dimensional ordered porous chitosan scaffold.

[0031] Measured results: Porosity: 62%...

Embodiment 3

[0035] 1) Weigh 25g of chitosan powder and dissolve it in dilute acetic acid with a volume fraction of 2%, to make a chitosan solution with a mass fraction of 5%, and let it stand for defoaming.

[0036] 2) uniformly coating the chitosan solution in step 1) on the surface of the mold, placing the mold in a KOH coagulation solution with a mass fraction of 5%, coagulating the chitosan at room temperature to form a film, and demoulding to obtain a chitosan film template ;

[0037] 3) pour the chitosan solution prepared in step 1) into the chitosan film template, seal, and immerse in a 5% KOH coagulation solution with a mass fraction, solidify for 4 hours, and demould to obtain a chitosan gel;

[0038] 4) The chitosan gel was washed with distilled water until neutral, dried in an oven at 80°C for 60 minutes, and then freeze-dried in a freeze dryer at -70°C for 72 hours to obtain a porous chitosan scaffold.

[0039] Measured results: porosity: 70%

[0040] Compression s...

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Abstract

The invention discloses a method for preparing three-dimensionally sequentially porous chitosan bone repair scaffold material. The method comprises the steps of dissolving chitosan powder in dilute acetic acid and standing to defoam; coating the prepared chitosan solution on the surface of a mold evenly, placing in a solidification solution at room temperature to solid and form a membrane, demoulding and obtaining a chitosan membrane moulding plate; pouring the prepared chitosan solution into the chitosan membrane moulding plate, sealing and soaking in the solidification solution to solid and demould to obtain chitosan gel; and washing the chitosan gel to be neutral by distilled water and drying. The method is simple in preparation technology; the three-dimensionally sequentially porous chitosan bone repair scaffold material prepared is good in regularity and high in strength and porosity, and can meet the requirements of tissue engineering scaffold material.

Description

technical field [0001] The invention relates to a preparation method of a bone repair support material, in particular to a preparation method of a three-dimensional ordered porous chitosan bone repair support material. Background technique [0002] Chitosan (Chitosan, CS) is a natural biodegradable polysaccharide, and its degradation product is weakly alkaline glucosamine, which is non-toxic and harmless to the human body and tissues. In addition to its excellent biocompatibility, it also has natural drug activity, anti-tumor activity, and anti-inflammatory effect. It can induce special cells for damaged organisms and accelerate wound healing. [0003] In the past, methods such as secondary freeze-drying and particle filtration were used to prepare porous chitosan scaffold materials. The obtained materials often have uneven pore size distribution, disordered structure, and low strength, which in turn affects the growth and diffusion of cells inside the scaffold, which is not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/56A61F2/82
Inventor 胡巧玲李愔喆王幽香
Owner ZHEJIANG UNIV
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