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Super macroporous polymer microspheres and preparation method thereof

A polymer, super-porous technology, applied in biochemical equipment and methods, alkali metal compounds, chemical instruments and methods, etc., can solve the problems of poor product stability, difficulty in large-scale separation and purification, and cumbersome preparation process.

Active Publication Date: 2013-10-30
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

although, The medium has a large pore size, but its preparation process is relatively cumbersome, and the product stability is poor, which is still difficult to meet the needs of large-scale separation and purification

Method used

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  • Super macroporous polymer microspheres and preparation method thereof
  • Super macroporous polymer microspheres and preparation method thereof
  • Super macroporous polymer microspheres and preparation method thereof

Examples

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preparation example Construction

[0060] (1) Preparation of primary emulsion:

[0061] Configure a certain concentration of metal ion salt solution as the inner water phase W 1 ; Dissolve a certain mass of linear polymer molecules in a benign organic solvent of the polymer to prepare an oil phase O; pour the inner aqueous phase solution into the oil phase, and emulsify through a homogeneous emulsifier or an ultrasonic cell disruptor to form W 1 / O primary emulsion.

[0062] The linear polymer described in the method includes at least one amphiphilic polymer, such as monomethoxy polyethylene glycol polylactic acid copolymer. In addition to amphiphilic polymers, it also contains one or more other polymers. The polymer molecular chain contains hydroxyl, carboxyl, epoxy, phenyl or chloromethyl, including methyl methacrylate and methacrylic acid Copolymer, polystyrene, polychloromethylstyrene or polybeta-hydroxyethylmethacrylate.

[0063] (2) Preparation of multiple emulsion:

[0064] One or more surfactants a...

Embodiment 1

[0073] Pour 500ml of 0.1% NaCl aqueous solution (W1) into 2L to dissolve 200g of methyl methacrylate and methacrylic acid copolymer P (MMA-MAA) (monomer molar ratio MMA / MAA is 20 / 1) and 8g of monomethyl methacrylate Ethyl acetate (oil phase, O) of oxypolyethylene glycol polylactic acid copolymer (PELA) (the ratio of polylactic acid and polyethylene glycol molecular block is 20 / 1), homogeneously emulsified at 24000rpm for 10min , temperature controlled in an ice-water bath, to obtain W 1 / O primary emulsion. Then the primary emulsion is poured into 25L of 2.5% polyvinyl alcohol (PVA) solution containing 0.01% NaCl, mechanically stirred at 1000rpm for 10min to obtain W 1 / O / W 2 Complex emulsion. After the double emulsion is formed, it is packaged in multiple cylindrical reagent bottles (the ratio of height to diameter is 10 / 1), and a vertical mixer is used to rotate at a constant speed of 60 rpm for 60 minutes, waiting for the aging of the double emulsion droplets. Pour the ...

Embodiment 2

[0076] Pour 500ml of 0.5% NaCl aqueous solution (W1) into 2L to dissolve 200g of methyl methacrylate and methacrylic acid copolymer P (MMA-MAA) (monomer molar ratio MMA / MAA is 20 / 1) and 8g of monomethyl methacrylate Ethyl acetate (oil phase, O) of oxypolyethylene glycol polylactic acid copolymer (PELA) (the ratio of polylactic acid and polyethylene glycol molecular block is 20 / 1), homogeneously emulsified at 24000rpm for 10min , temperature controlled in an ice-water bath, to obtain W 1 / O primary emulsion. Then the primary emulsion is poured into 25L of 2.5% polyvinyl alcohol (PVA) solution containing 0.01% NaCl, mechanically stirred at 1000rpm for 10min to obtain W 1 / O / W 2 Complex emulsion. After the double emulsion is formed, it is packaged in multiple cylindrical reagent bottles (the ratio of height to diameter is 10 / 1), and a vertical mixer is used to rotate at a constant speed of 60 rpm for 60 minutes, waiting for the aging of the double emulsion droplets. Pour the ...

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Abstract

The invention provides super macroporous polymer microspheres and a preparation method thereof. The preparation method comprises the following steps of: firstly, preparing an oil-in-water in-water composite emulsion as a template for super macroporous microspheres through a two-step emulsion process; then, solidifying an oil phase by using a solvent removal method to form super macroporous microspheres provided with inner-outer through pore passages; and finally, after molding the microspheres, further crosslinking microsphere skeleton molecules to obtain microspheres with rigid resin structures. The microspheres prepared by the method have a through pore passage structure, the controllable particle size range is 0.1-300 microns, the controllable pore size range is 0.09-90 microns, and the controllable porosity range is 10-90%. Super macroporous structures are beneficial for biological macromolecules to penetrate through and enter the microspheres, the mass transfer by convection in the microspheres can be realized, and the rigid structure can tolerate higher pressure and higher flow velocity. The super macroporous polymer microspheres can be used as stationary phase fillers for chromatographic separation, immobilized carriers of enzymes, cell culture micro-carriers, tissue engineering micro scaffold materials, adsorbing materials and the like.

Description

technical field [0001] The invention belongs to the interdisciplinary field of polymer materials and biochemical industry, in particular, the invention relates to a super-macroporous polymer microsphere and a preparation method thereof. Background technique [0002] With the rapid development of biotechnology, more and more biochemical products are moving from the laboratory to the market, and a wide variety of biological macromolecules such as proteins, peptides, and nucleic acids need to be produced, purified, and formulated. In the preparation process of biochemical products, separation and purification are often the key links that restrict product quality and cost. Among many purification methods, liquid chromatography is by far the most effective means of separation and purification, and its biocompatibility guarantees a high activity yield. With the industrialization of biotechnology, the requirements for chromatographic efficiency and chromatographic capacity are get...

Claims

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Application Information

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IPC IPC(8): C08J9/36C08J9/28C08J3/24C08J3/16B01J20/26B01J20/28B01J20/30B01J20/285C12N11/08C12N5/00A61L27/18A61L27/16A61L27/50A61L27/56
Inventor 马光辉高飞陈松飞苏志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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