Preparation method of vinyl collagen

A collagen and protoprotein technology, applied in the field of vinyl collagen preparation, can solve the problems of collagen thermal stability and poor physical and mechanical properties, and achieve the effects of low equipment requirements, simple operation and mild reaction conditions

Inactive Publication Date: 2016-09-14
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing vinyl collagen, which solves the problem of...

Method used

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  • Preparation method of vinyl collagen
  • Preparation method of vinyl collagen
  • Preparation method of vinyl collagen

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preparation example Construction

[0024] A kind of preparation method of vinyl collagen of the present invention, specifically implement according to the following steps:

[0025] Step 1: Weigh collagen (weight average molecular weight 4825, 145508 or 181266) and mix it with phosphate buffer solution (pH 7.4), stir at 70°C ± 2°C for 0.5-1 hour to fully dissolve it, and obtain Collagen solution. Wherein, the mass volume ratio of collagen to phosphate buffer solution is 1:8˜1:12 (g / mL).

[0026] Step 2, put the collagen dissolved in step 1 in a water bath environment at a temperature of 30-80° C., add methacrylic anhydride with a mass concentration of 94% at a rate of 1 mL / min, and stir for 1-6 hours to obtain Collagen solution after modification; wherein, the volume-mass ratio of methacrylic anhydride to collagen is 0.1:5-2.4:5 (mL / g).

[0027] Step 3, place the collagen solution modified in step 2 in a dialysis bag with a suitable molecular weight (500D or 3500D), use deionized water as the dialysate, and di...

Embodiment 1

[0034] Step 1, weighing 5 g of collagen with a weight average molecular weight of 181266 and mixing it with 50 mL of phosphate buffer solution (pH 7.4), stirring at 70° C. for 0.5 h to fully dissolve it.

[0035] Step 2, put the collagen solution in step 1 in a water bath environment at a temperature of 40° C., add 0.1 mL of methacrylic anhydride to it at a rate of 1 mL / min, stir and react for 2 hours, and obtain vinyl collagens with different degrees of substitution ( CMA) solution.

[0036] Step 3, put the CMA solution in step 2 into a suitable dialysis bag with a molecular weight of 3500D, use deionized water as the dialysate, dialyze at 40°C for 24 hours, and place the dialyzed collagen solution in a freeze dryer , pre-frozen at -20°C, and then freeze-dried for later use.

Embodiment 2～ Embodiment 9

[0038] Referring to Example 1, the difference from Example 1 is that in step 2, the amount of methacrylic anhydride added is 0.3mL, 0.6mL, 0.9mL, 1.2mL, 1.5mL, 1.8mL, 2.1mL and 2.4mL.

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PUM

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Abstract

The invention discloses a preparation method of vinyl collagen. The method includes: firstly dissolving collagen; and then conducting graft modification on the collagen. The invention employs methacrylic anhydride for graft modification of the collagen, the operation is simple, the reaction time is short, and the reaction conditions are milder. The equipment and medicines involved in the invention have the characteristics of low equipment requirements, low cost, and strong operability. The vinyl collagen prepared by the method provided by the invention lays the foundation for film forming crosslinking of collagen itself, graft crosslinking of other reactive high polymer materials and the like.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a preparation method of vinyl collagen. Background technique [0002] Collagen, also known as collagen, is the most abundant protein in mammals, accounting for about 25% to 30% of the total protein. Collagen has been widely used due to its excellent low immune activity, biocompatibility and biodegradability, but unmodified collagen often has disadvantages such as poor thermal stability, fast degradation rate and low mechanical strength. Can not meet the needs of many usage environments. With the increasing diversity of functional requirements, the modification of collagen has become a research hotspot. By modifying collagen, the thermal stability and mechanical properties of collagen can be improved, and the degradation rate of collagen can be reduced. [0003] At present, the modification of collagen can be roughly divided into cross-linking modification...

Claims

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Application Information

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IPC IPC(8): C08H1/00
CPCC08H1/00
Inventor 徐娜王学川任龙芳马向东强涛涛石叶叶董佳琳
Owner SHAANXI UNIV OF SCI & TECH
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