GelMA hydrogel human acellular amniotic membrane three-dimensional two-layer dressing preparation method

Abstract

The invention relates to the field of medical biological dressings, in particular to a GelMA hydrogel human acellular amniotic membrane three-dimensional two-layer dressing preparation method which isa method for preparing a GelMA hydrogel/human acellular amniotic membrane three-dimensional two-layer dressing through integration of electrostatic spinning and photo-crosslinking. The method includes: subjecting gelatin to MA (methylacrylic acid) graft modification to form GelMA, covering D-HAM (decellularized human amniotic membrane) with GelMA in a porous fiber form through electrostatic spinning to obtain the three-dimensional two-layer bioactive dressing, and subjecting the dressing to photo-crosslinnking with a photoinitiator Irgacure2959; lyophilizing the dressing subjected to photo-crosslinnking, performing gamma irradiation sterilization, and packaging under sterile conditions. Great anti-inflammation and anti-infection effects and high biocompatibility are achieved, and quick healing of oral mucous wounds can be promoted.

Description

technical field [0001] The invention relates to the field of medical biological dressings, in particular to a preparation method of a GelMA hydrogel human decellularized amniotic membrane three-dimensional double-layer auxiliary material. Background technique [0002] The position of the oral mucosa is relatively special. It covers the surface of the oral cavity. The front is connected to the skin of the lips and the rear is connected to the mucous membrane of the throat. It plays an oral protective role by resisting mechanical stimulation, limiting toxic substances, microbial invasion and saliva secretion. Severe mucosal lesions, tumor surgery, etc. have severe trauma to the oral mucosa, often causing large oral mucosal defects. In the past, iodoform oil gauze was used to cover the wound, and adjacent flaps were transferred and repaired, such as buccal fat pad flap, palatine flap, platysma flap etc., not only increase the difficulty and trauma of the operation, but also cau...

AI Technical Summary

Problems solved by technology

However, the decellularized amniotic membrane is a single-layer amniotic membrane, and its disadvantages are: thin tissue, poor mechanical properties, easy to tear, easy to curl, difficult to cut into shapes, and easy to dissolve after covering the wound

However, GelMA hydrogel itself lacks biologically active substances and cannot promote the rapid repair of oral mucosal injuries. GelMA hydrogel alone is rarely used to repair oral mucosal injuries, let alone GelMA through electrospinning and photocrosslinking of decellularized amniotic membrane. Invention Report on Preparation of Bilateral Bioactive Dressing