Traditional Chinese medicinal exosome-loaded compound dressing with effects of promoting wound healing and regeneration and preparation method of compound dressing

A wound healing and exosome technology, applied in medical science, bandages, etc., to achieve the effects of induced migration, good viscoelasticity and mechanical strength, and a wide range of applications

Active Publication Date: 2020-02-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after loading traditional Chinese medicine exosomes and cyt...

Method used

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  • Traditional Chinese medicinal exosome-loaded compound dressing with effects of promoting wound healing and regeneration and preparation method of compound dressing
  • Traditional Chinese medicinal exosome-loaded compound dressing with effects of promoting wound healing and regeneration and preparation method of compound dressing
  • Traditional Chinese medicinal exosome-loaded compound dressing with effects of promoting wound healing and regeneration and preparation method of compound dressing

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Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1: A kind of compound dressing that promotes wound healing and regeneration and loading traditional Chinese medicine exosomes and preparation method, comprises the following steps:

[0051] 1) react gelatin with methacrylic anhydride, then dialyze in a 8k-14kDa dialysis bag at room temperature for 1 week, and then freeze-dry to prepare methacrylated gelatin;

[0052] 2) First wash the privet, squeeze the juice, and pass through a 100-mesh sieve; then through the differential centrifugation method, centrifuge at 1000g and 12000g successively, take the supernatant and centrifuge at 100000g with an ultracentrifuge, and then use the Resuspended in PBS. Prepare a density gradient solution with sucrose, and then ultracentrifuge at 200,000g for 60 minutes; then take exosomes of different components and centrifuge at 50,000g for 60 minutes to prepare traditional Chinese medicine exosomes;

[0053]3) Dissolve methacrylated gelatin in PBS to make the concentration 5%;...

Embodiment 2

[0058] Embodiment 2: A kind of compound dressing and preparation method that promotes wound healing and regeneration and loading traditional Chinese medicine exosomes, comprise the following steps:

[0059] 1) reacting gelatin with methacrylic anhydride, then dialysis with 8k-14kDa dialysis bag at room temperature for 2 weeks, and then freeze-drying to prepare methacrylated gelatin;

[0060] 2) Wash the salvia miltiorrhiza, squeeze the juice, and pass through a 200-mesh sieve; then through the differential centrifugation method, centrifuge at 2000g and 10000g successively, take the supernatant and centrifuge at 120000g with an ultracentrifuge, and then wash the precipitate with PBS Resuspended. Use CsCl gradient solution, and then ultracentrifuge at 100,000g for 60 minutes; then take exosomes of different components and centrifuge at 100,000g for 60 minutes to prepare traditional Chinese medicine exosomes;

[0061] 3) Dissolve methacrylated gelatin in PBS to make the concentr...

Embodiment 3

[0067] Embodiment 3: A kind of compound dressing that promotes wound healing and regeneration and loading traditional Chinese medicine exosomes and preparation method, comprises the following steps:

[0068] 1) react gelatin with methacrylic anhydride, then dialyze in a 8k-14kDa dialysis bag at room temperature for 2 weeks, and then freeze-dry to prepare methacrylated gelatin;

[0069] 2) First wash the saffron, squeeze the juice, and pass through a 150-mesh sieve; then through the differential centrifugation method, centrifuge at 3000g and 5000g successively, take the supernatant and centrifuge at 100000g in an ultracentrifuge, and then separate the precipitate Resuspend in ultrapure water. Use a sucrose gradient solution, and then ultracentrifuge at 100,000g for 60 minutes; then take exosomes of different components and centrifuge at 10,000g for 60 minutes to prepare traditional Chinese medicine exosomes;

[0070] 3) Dissolve methacrylated gelatin with ultrapure water to ma...

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Abstract

The invention provides traditional Chinese medicinal exosome-loaded compound dressing with effects of promoting wound healing and regeneration and a preparation method of the compound dressing. The preparation method comprises the following steps: preparing methacrylate esterified gelatin by a reaction of gelatin and methacrylic anhydride; preparing a traditional Chinese medicinal exosome throughdifferential centrifugation; combining the prepared exosome with the methacrylate esterified gelatin, adding SDF-1 and photosensitizer into the mixture to prepare a pre-gel system; and forming the compound dressing through ultraviolet crosslinking. The dressing is novel photosensitive dressing of a compound traditional Chinese medicinal exosome, the compound can be formed through ultraviolet crosslinking in vitro or in-situ crosslinking on a wound after the pre-gel system is formed. The compound dressing is quick in crosslinking, is simple and convenient, can be used for effectively protectinga wound area, inducing migration of mesenchymal stem cells and promoting wound heating and regeneration with the traditional Chinese medicinal exosome, and can effectively solve the problem in current wound healing and regenerating process. Therefore, the compound dressing has a wide application value in wound healing and regeneration.

Description

technical field [0001] The invention relates to a composite dressing that promotes wound healing and regeneration and loads traditional Chinese medicine exosomes and a preparation method. Background technique [0002] The skin is the first line of defense of the human body and is also the largest organ of the human body. It has multiple functions such as sensation and barrier. Skin can form an effective barrier between organisms and the environment, preventing the invasion of pathogens, resisting chemical and physical attacks, etc. However, in daily life, the skin is often affected by a variety of external factors, causing trauma, and the loss of a large area often has serious consequences, resulting in severe disability or even death, which brings heavy pressure and burden to individuals and society. [0003] Wound healing is a complex and dynamic process, including the joint regulation of various tissues and cells. The uncontrolled regulation of the wound repair process ...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0038A61L26/0047A61L26/0066A61L26/008A61L2300/252A61L2300/30A61L2300/412A61L2300/45
Inventor 彭丽华许学寒
Owner ZHEJIANG UNIV
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