107 results about "Biomaterial preparation" patented technology

The invention discloses a preparation method of high-purity red date polysaccharide nano-selenium, and belongs to the field of biological material preparation. The preparation method of the high-purity red date polysaccharide nano-selenium comprises the following steps: adding water to dissolve red date polysaccharide to obtain a red date polysaccharide solution, then mixing the red date polysaccharide solution with a nano-selenium solution, reacting to obtain a mixed solution, and separating and purifying to obtain the red date polysaccharide nano-selenium. According to the preparation method disclosed by the invention, high-purity red date polysaccharide nano-selenium can be efficiently and stably prepared, the product has huge application potential in the fields of biomedicine, functional food and the like, and powerful technical support is provided for the development of related industries. Compared with a conventional process in the prior art, the preparation process for preparing the red date polysaccharide and the nano-selenium first and then compounding the red date polysaccharide and the nano-selenium to obtain the final product has obvious performance advantages in the aspects of product purity, storage stability and the like, and powerful technical support is provided for further application and development of the red date polysaccharide nano-selenium.

The invention provides a biological material for repairing an ovarian function as well as a preparation method and application thereof, and relates to the technical field of biological materials, a large number of experiments prove that specific biological protein components for reversing the ovarian function in extracellular vesicles are growth differentiation factor 9 (GDF-9) for repairing an ovary, and the biological material has the advantages that the ovarian repairing function mechanism of the specific biological protein components for reversing the ovarian function in the extracellular vesicles is found; according to the present invention, the GDF-9 extracellular vesicles (GDF-9Over-existed Extracellular vesicles, GOEs) are adopted as the active substance, such that the component is effectively extracted and purified so as to achieve the target of targeted therapy.

The invention belongs to the technical field of preparation of biological materials, and particularly discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application of the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The preparation method comprises the following steps: dissolving freeze-dried sea cucumber flower powder in deionized water, adjusting the pH value to be alkaline, heating and stirring, centrifuging, discarding the precipitate, adjusting the pH value to be acidic, centrifuging, collecting the precipitate, and freeze-drying to obtain sea cucumber flower protein powder; preparing a reaction solution, adding simulated gastric juice and simulated intestinal juice, stirring for reaction, inactivating protease, centrifuging, taking supernatant, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; the preparation method comprises the following steps: preparing a solution from sea cucumber, adding FeCl2. 4H2O and ascorbic acid, adjusting the pH value to be acidic, carrying out heating reaction, centrifuging, discarding the supernatant, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The invention discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application thereof. The carrier can promote absorption of an organism to iron, a relatively high iron ion release rate is maintained in an intestinal environment, and the in-vivo bioavailability of iron ions is improved.

The invention relates to the technical field of biological material preparation, and particularly discloses a method and a system for industrial continuous production of silk fibroin. In order to solve the problems of high energy consumption, heavy pollution, low efficiency, poor product stability and the like in the prior art, efficient removal of sericin is realized through a gradient degumming synergistic treatment technology (high-temperature and high-pressure alkali treatment and directional enzymolysis), and desalination and concentration are synchronously completed in combination with a membrane separation directional purification technology (a hollow fiber ultrafiltration system). The system integrates the gradient degumming module, the dissolution-filtration coupling module, the membrane separation module and the resource circulation subsystem, the technological parameters (degumming rate, viscosity and transmembrane pressure difference) are regulated and controlled in real time through the intelligent control center, the continuous production stability is ensured, the purity of the obtained silk fibroin solution is larger than or equal to 99%, and the standard of beauty makeup materials is met. The method and the system for industrial continuous production of silk fibroin have the advantages of high efficiency, low consumption and environmental protection, and are suitable for large-scale production.

The invention relates to a bioink formulation that is prepared using a cellulose biomaterial that is derived from plant cells. The cellulose biomaterial is a scaffold such as an extracellular matrix derived from plant cells such as live plant cells, dead plant cells, decellularized plant cells or a plant cell-line that could be used to develop a biostable, biodegradable, bioprintable and tunable bioinkthat could be used for a variety of applications including bioprinting. A method of purifying the cellulose biomaterial by an acidic maceration and decelullarization technique is also provided along with a method of preparing the tunable bioink from the derived plant biomaterial.

The invention relates to the technical field of preparation and application of a co-polymer biological material, and discloses a matrix-cell co-polymer biological material, which comprises a matrix-cell co-polymer, and the matrix-cell co-polymer comprises human decellularized alveolar bone matrix microparticles and human dental sac stem cells. According to the matrix-cell copolymer biological material as well as the preparation method and the application thereof, hDABMPs capable of providing a tissue-specific microenvironment and hDFSCs with development potential are utilized to prepare a developmental bionic matrix-cell copolymer biological material, and the developmental bionic matrix-cell copolymer biological regeneration material has good mechanical strength, good mechanical properties, good biocompatibility, good biocompatibility and good biocompatibility. The stem cell polymer carrying the tissue-specific microenvironment and the developmental potential provides key biological signals of a regeneration scaffold and the specific tissue microenvironment for tissue regeneration so as to support and enhance the regeneration capacity of the implanted stem cell polymer. The strategy highlights the synergistic effect of development guidance and microenvironment induction.

The present invention discloses a method for preparing food-grade glassine paper from edible mushroom liquid fermentation mycelium and the food-grade glassine paper, which relates to a method for preparing glassine paper using natural biological materials. The preparation method of the food-grade glassine paper is as follows: Step 1: Preparation of the base paper of edible mushroom liquid fermentation mycelium; Step 2: Preparation of the surface paper of edible mushroom liquid fermentation mycelium; Step 3: Preparation of the food-grade glassine paper. The present invention prepares the food-grade glassine paper from edible mushroom liquid fermentation mycelium, which can replace the traditional papermaking method mainly using wood as the raw material, without adding plastics and chemical coating agents, and has the advantages of environmental protection, low carbon and degradability. It is a food-grade oil-proof, moisture-proof and semi-transparent glassine paper, and has broad application prospects in various fields such as food, medicine and express labels.

The invention discloses a phosphite dehydrogenase mutant, a biological material, a preparation method and application, and compared with wild phosphite dehydrogenase, the phosphite dehydrogenase mutant has one or more amino acid residue mutations in the 29th site, the 31st site, the 59th site, the 68th site, the 185th site and the 275th site. According to the mutant, the regeneration efficiency of reduced coenzyme NADH is effectively improved, and the catalytic efficiency of the mutant N29T / Q185C / K275E is 1.7 times that of wild type phosphite dehydrogenase; the mutant is mild in reaction condition and wide in range, the reaction temperature is 15-40 DEG C, the reaction pH is 4-10, the stability is improved compared with that of wild phosphite dehydrogenase, and the mutant has high application value.

The invention discloses preparation and application of a biological material for targeted therapy of osteoarthritis, and the preparation method comprises the following steps: according to a phage screening principle, after phages are combined with cartilage cells, washing off phages which are not combined with the cartilage cells, are not firmly combined with the cartilage cells and are non-specifically combined with the cartilage cells; eluting the specifically bound bacteriophage with glycine acid; after the specific binding bacteriophage is amplified by escherichia coli, screening is carried out by utilizing a bacteriophage display technology and cartilage cells; the preparation method comprises the following steps: preparing a small-size porous microgel loaded drug by adopting a multiple Pickering emulsion template method; and grafting the screened cartilage targeting peptide to the surface of the porous hydrogel microsphere to obtain the HS-SP microsphere. According to the invention, the problem of OA targeting can be effectively solved by adopting a phage display technology, and a drug is precisely targeted and delivered to an OA lesion region; the porous hydrogel microspheres with small particle size are prepared by adopting a multiple Pickering emulsion method, so that the use of a large number of surfactants is avoided, and the efficient loading and release of drugs can be realized.

The invention belongs to the technical field of medical biological material preparation, and particularly relates to a sterile medical wound repair hydrogel dressing which takes dual-modified hyaluronic acid, polyvinyl alcohol, nano zinc, a humectant and a lubricant as main raw materials. The preparation method comprises the following steps: firstly, utilizing dual-modified hyaluronic acid as a core skeleton of a hydrogel system, and jointly constructing a double-layer network structure through free radical polymerization and crosslinking of boric acid ester bonds and carbon-carbon double bonds formed between phenylboronic acid groups and polyvinyl alcohol; meanwhile, polyvinyl alcohol and zinc ions form coordinate bonds which are interpenetrated in a network, so that the hydrogel dressing with a triple network structure is formed. The unique network structure can simulate an extracellular matrix microenvironment and is beneficial to promoting cell proliferation. In the using process, the hydrogel dressing can play a role in integrating multiple functions. Firstly, the hydrogel dressing can be attached to the surface of the skin to form a breathable physical barrier, meanwhile, invasion of bacteria is effectively isolated, secondly, moisture of the skin can be supplemented through the moisturizing performance of the hydrogel dressing, and due to the flexibility and elasticity of the hydrogel dressing, the use comfort of the hydrogel dressing is further improved, and painless dressing change is achieved. More importantly, long-acting release of the zinc ions can inhibit bacterial infection at the wound, promote wound repair and accelerate wound healing.

The invention provides recombinant III-type collagen and application thereof, and relates to the technical field of genetic engineering. According to the invention, the yield of the collagen is improved by optimizing the nucleotide sequence of the target gene and optimizing the culture conditions of pichia pastoris, so that the collagen is efficiently expressed in engineering bacteria. The recombinant engineering bacterium shows strong potential in the aspect of improving the yield of protein with high economic value, and is suitable for large-scale industrial production. The recombinant III-type collagen is high in expression quantity, simple in preparation process and suitable for preparation of biological materials for medical cosmetology, wound repair and the like, and has a wide application prospect.

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.

The invention belongs to the technical field of biological material preparation, and particularly relates to a preparation method of a self-oxygen-supply hydrogel cell scaffold, an obtained product and application. According to the invention, stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres are realized. The method is realized by the following steps: adding compounds P-MA, P-Cys, ColMA and PEG-CaO2 into a PBS (Phosphate Buffer Solution), adding a photoinitiator, and irradiating and curing by ultraviolet light with the wavelength of 365nm to obtain the self-oxygen-supply composite hydrogel. The invention provides a novel method for applying composite hydrogel loaded with oxygen-producing nano-particles to 3D cell sphere culture and continuously releasing O2 in a slightly acidic environment to solve the problems of death of intermediate cells caused by oxygen deficit in the 3D culture process of existing cell spheres, indefinite components of matrigel, high price and the like. The problem of internal oxygen deficit in the cell ball culture process is solved, and the gel component is defined to be used for researching the effect between other components and cells. In addition, the self-oxygen-supply hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

The present invention belongs to the field of biomaterial preparation, and discloses a thermosensitive hydrogel material and a preparation method thereof. The preparation method comprises: (1) adding chitosan to a dilute acetic acid solution, stirring and ultrasonically dispersing, to obtain solution A; (2) adding anhydrous citric acid to solution A, heating in a water bath, to obtain solution B; (3) dissolving gum arabic in deionized water, to obtain solution C; (4) adding solution C to solution B, blending and dispersing, and heating in a water bath, then pouring it into a 24-well plate, adding a certain amount of cross-linking agent, standing, to obtain a chitosan / anhydrous citric acid / gum arabic thermosensitive hydrogel material. The thermosensitive hydrogel preparation method adopted does not generate pollutants, is green and environmentally friendly, and is simple to operate; the prepared hydrogel material has chemical bonds formed by cross-linking inside, has good mechanical properties, has been greatly improved in terms of strength, biocompatibility, etc., has better mechanical strength and ductility, and is thermosensitive.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The invention relates to the technical field of biological material preparation, in particular to a method for preparing carboxymethyl chitosan through irradiation treatment of chitin-enriched biomass and application. According to the method, chitin-enriched biomass is taken as a raw material and is sequentially subjected to pretreatment, irradiation pretreatment, decalcification treatment, degreasing and deproteinization, deacetylation and carboxymethylation; the irradiation absorbed dose of the irradiation pretreatment is greater than 0 and less than or equal to 1000kGy. According to the method, the structure porosity of chitin is improved through high-energy ray irradiation treatment, the permeation efficiency of subsequent reactions such as decalcification and deproteinization is remarkably improved, meanwhile, the dosage of chemical reagents is reduced, and the substitution degree and water solubility of the product are improved by further combining a multi-step synergistic chemical modification process such as freezing alkalization and gradient dialysis purification technologies; the molecular weight distribution is uniform. The high-purity carboxymethyl chitosan provides technical guarantee for development of novel medical materials.

This invention relates to the field of biomaterial preparation technology, and in particular to a morphology-controllable mesoporous hydroxyapatite nanosphere, its preparation method, and its applications. The preparation method includes: S1, mixing an aqueous solution of pyridoxal phosphate with an aqueous solution of calcium chloride, adjusting the pH of the mixed solution to 7-11 to obtain a precursor solution; S2, placing the precursor solution in a microwave reactor for reaction to obtain mesoporous hydroxyapatite nanospheres. The preparation method provided by this invention is simple, the reaction conditions are easily controlled, and it is highly safe and reliable. It enables precise control of the morphology and particle size of the hydroxyapatite microspheres, and the prepared product exhibits excellent biocompatibility and structural regularity. It has good industrial application prospects in the field of biomedical implants such as bone tissue repair and is suitable for large-scale production.

The present invention relates to the technical field of biomaterial preparation, and in particular to an adhesive hydrogel with anti-inflammatory, antioxidant and pro-angiogenic capabilities and its application. The adhesive hydrogel of the present invention comprises an anti-adhesion layer, an adhesion layer and an inner layer arranged in sequence; the anti-adhesion layer and the adhesion layer are respectively formed by cross-linking ACG solutions of different pH values; the inner layer uses methacrylate whey protein as a base material and is loaded with EGCG@DEX‑PBAP. The adhesive hydrogel can be used for the repair of skin damage, muscle damage, nerve damage, and myocardial damage tissue.

The invention belongs to the technical field of biological material preparation, and particularly relates to a copper-based metal organic framework nanoparticle as well as a preparation method and application thereof. The invention relates to a copper-based metal organic framework nano-particle which is obtained by carrying out hydrothermal reaction on a mixed solution, polyvinylpyrrolidone, 1, 3, 5-benzene tricarboxylic acid and a copper compound, the mixed solution is obtained by mixing deionized water, absolute ethyl alcohol and N, N-dimethylformamide; the ratio of the polyvinylpyrrolidone to the mixed solution is (70mg-90mg): 9mL; the ratio of the 1, 3, 5-benzene tricarboxylic acid to the copper compound to the mixed solution is (7g-14g): 7g: 1500mL. The particle size of the copper-based metal organic framework nano-particles is 167 nm, the loading rate of the copper-based metal organic framework nano-particles to adriamycin within 24 h reaches 78.3%, the copper-based metal organic framework nano-particles have the pH response drug release characteristic in a tumor microenvironment with the pH being 5.2, the cumulative release rate within 72 h reaches 54.75%, and the cumulative release rate is increased by 6.3 times compared with the physiological condition with the pH being 7.4. Complete cell internalization is realized within 6 hours, and acid-triggered drug release is promoted.

This invention discloses a bimetallic complex with whitening and anti-aging effects, its preparation method, and its application, belonging to the field of biomaterial preparation technology. The preparation method includes S1, dissolving an amino acid in water to obtain solution A; S2, dissolving a metal salt in ethanol to obtain solution B; S3, mixing solutions A and B evenly, and then reacting them through a solvothermal reaction to obtain solution C; S4, washing and drying solution C to obtain the final product. The amino acid in step S1 is at least one of glutamic acid and aspartic acid; the metal salt in step S2 is copper acetate and zinc acetate, copper acetate and manganese chloride, or copper acetate and cobalt nitrate; the solvothermal reaction conditions in step S3 are: temperature 60-110℃, reaction time 2-48h. The prepared bimetallic complex has both whitening and anti-aging effects, providing a new direction for the application of BioMOFs materials.

The invention belongs to the technical field of nano biological material preparation, and discloses a tumor targeting peptide modified gold-copper bimetallic cluster nano enzyme and a preparation method and application thereof, and the preparation method comprises the following steps: preparing the gold-copper bimetallic cluster nano enzyme by using natural protein; the tumor targeting peptide is modified on the surface of the gold-copper bimetallic cluster nano-enzyme through amidation reaction to obtain the tumor targeting peptide modified gold-copper bimetallic cluster nano-enzyme. The gold-copper bimetallic cluster nano-enzyme obtained by the invention has excellent biological safety, can specifically aggregate in tumor cells and induce copper death, further realizes a remarkable tumor cell growth inhibition effect on the basis of a synergistic effect of enzyme-like catalysis, and has a wide application prospect in the field of tumor treatment.

The invention discloses a method for improving mineralized collagen performance based on discrete increase of ion concentration, and belongs to the technical field of biological material preparation, and the method comprises the following steps: step 1, preparing a reaction stock solution; 2, preparing a dynamically mineralized collagen solution; and step 3, preparing the bracket with dynamically changed ion concentration. According to the method for improving the performance of the mineralized collagen based on discrete increase of the ion concentration, the nonlinear process of mineralization of natural bones is simulated by dynamically regulating and controlling the ion concentration, more inorganic minerals can be attached to the collagen, and the mineralization degree of the prepared collagen is higher on the micron scale; on the macroscopic scale, the mechanical property of the mineralized collagen is better, the mineral substances are more, and the technical support can be provided for preparing the multi-scale bionic bone matrix material.

Provided are a biological material for restoring ovarian functions, a preparation method therefor and the use thereof, relating to the technical field of biological materials. A large number of experiments verify that: it is found that a specific biological protein component for reversing ovarian functions in extracellular vesicles is the growth differentiation factor 9 (GDF-9), and the mechanism of action thereof for restoring the ovary is determined, and therefore extracellular vesicles (GOEs) that overexpress GDF-9 serve as an active substance, and by means of extraction and purification of said component, the purpose of targeted therapy is achieved.

The application discloses a PAM-AA-NHS-based multifunctional biomimetic polymer adhesive for wound repair and a preparation method thereof, and belongs to the field of biomaterial preparation and biomedical application. N-hydroxysuccinimide acrylate copolymerized polyacrylamide hydrogel is used as a substrate and as a main chain network, and functionalized materials tannic acid and phenolic hydroxyl chitosan containing o-diphenol groups are introduced, so that effective adhesion can be formed at a biological tissue interface, and cross-linking can be generated with the main chain network through hydrogen bond action to form a secondary network. In addition, aldehyde group-rich oxidized dextran side chains are introduced, which can form Schiff base cross-linking with the main chain network and amino groups in the biological tissue interface to form long-acting adhesion performance. The adhesive has good tissue adhesion, biocompatibility, antibacterial activity and wound healing promotion capacity, can replace sutures, has a positive effect on the formation of new tissues and tissue healing, and is expected to be applied in clinical treatment of wound surfaces.

The invention relates to the technical field of biological material preparation, and particularly discloses hyaluronic acid hydrogel as well as a preparation method and application thereof in preparation of a corneal injury repair material. The preparation method of the hyaluronic acid hydrogel comprises the following steps: S1, preparing a hyaluronic acid solution; s2, adding a cross-linking agent into the hyaluronic acid solution, and adjusting to acidic pH to obtain a solution A; s3, adding a carboxyl activator into the solution A, and stirring to obtain hyaluronic acid hydrogel; a preparation method of the hyaluronic acid solution in the step S1 comprises the following steps: taking hyaluronic acid, adding the hyaluronic acid into the traditional Chinese medicine extracting solution, and uniformly stirring to obtain the hyaluronic acid solution. Researches show that the hyaluronic acid hydrogel prepared by the method disclosed by the invention has the effect of promoting proliferation of corneal epithelial cells; therefore, the material has important application value when being used as a corneal injury repair material.

The invention relates to a method for preparing pH / GSH-responsive drug-loaded biodegradable targeted nanoparticles based on dual-template molecular imprinting polymers. The invention belongs to the field of functional material preparation and relates to a method for preparing functional monomers prepared from biomaterials and biodegradable molecular imprinting polymer nanoparticles with transferrin and tumor markers as dual-template molecules. The invention solves the problems of poor biocompatibility of molecular imprinting technology, difficulty in degradation, low drug loading and encapsulation rate, few types of functional monomers, unclear degradation mechanism or difficulty in degradation, and insufficient interaction with target molecules. Methods: 1. Preparation of SS-DMONs; 2. Loading of anticancer drugs; 3. Preparation of Cys-DA; 4. Preparation of dual-template molecular imprinting nanopolymers. The invention increases the types of functional monomers of molecular imprinting polymers, has better safety and biocompatibility, higher drug loading capacity and stronger targeting effect.

The invention discloses porous biological ceramic as well as a preparation method and application thereof, and belongs to the technical field of biological material preparation. The preparation method comprises the following steps: dropwise adding a phosphorus-containing compound solution into a calcium-containing compound solution under the condition that the pH value is 6-12, reacting to generate a biological ceramic precursor, centrifuging, drying, calcining, grinding and sieving the precursor to obtain biological ceramic powder, and then mixing the powder with a dispersing agent, a binder and water to prepare biological ceramic slurry; mixing the slurry with a pore-forming agent, and drying to obtain a biological ceramic blank; and finally, shaping and sintering the blank body to obtain the porous biological ceramic. According to the method, regulation and control of the pH value of a reaction system are taken as a main means, and the calcination temperature of the precursor is controlled, so that continuous, accurate and repeatable control on the proportion of two phases of hydroxyapatite and beta-tricalcium phosphate in the biological ceramic can be realized.

The present invention relates to a crosslinkable polymer composition comprising at least one calcium phosphate, at least one biocompatible and biodegradable organic polymer or one of its precursors, and Zn-ZnO core-shell particles, a biomaterial obtainable by photopolymerization of such a crosslinkable polymer composition, methods for preparing such a composition and such a biomaterial, and the use of such a biomaterial for various applications in the health sector.

The present invention discloses an injectable dual - biomimetic bone adhesive for promoting fracture healing and its preparation method, belonging to the field of biomaterial preparation. Inspired by the underwater adhesion mechanisms of marine mussels and barnacles, it uses biomimetic marine mussel substances (such as tannic acid), biomimetic marine barnacle substances (such as sodium alginate), silk fibroin, calcium chloride, and magnesium particles to form a dual - biomimetic bone adhesive with wet adhesion. Based on the hydrogen bonds, metal - phenol coordination bonds, electrostatic interactions, and β β - sheet protein folding conformations formed among the components, the obtained dual - biomimetic bone adhesive not only has good biocompatibility, excellent wet adhesion, and mechanical properties matching bone tissue strength, can be used for fracture bone fixation and repair, but also has the ability of in - situ gelation and can be directly injected at the injury site. The raw materials used in the adhesive prepared by the present invention are safe and widely sourced. Therefore, it is expected to achieve potential clinical application value in the fixation and repair of bone injuries.

The invention belongs to the field of biological material preparation, and particularly relates to degradable drug-loading hydrogel and a preparation method thereof.Acrylate groups are introduced into 6s-PLA, 6s-PLA-HA is synthesized, a hydrogel material is synthesized through a photopolymerization method, then cefprozil serves as a model drug, and drug loading is conducted on the hydrogel through the supercritical impregnation technology. The composite material is self-prepared six-arm star-shaped polylactic acid (6s-PLA), the 6s-PLA is terminated by using acryloyl chloride to synthesize 6s-PLA-HA, PLA-PEG hydrogel is prepared by using a photopolymerization method, and a hydrogel material loaded with cefprozil is prepared by using a supercritical carbon dioxide impregnation technology. The material has the advantages of being excellent in mechanical property, non-toxic, good in biological safety, non-irritant, easy to process and form, degradable, anti-inflammatory and the like, and is expected to be widely applied to the field of biomedical materials.