11 results about "Biomaterial preparation" patented technology

This invention relates to the field of biomaterial preparation technology, and in particular to a morphology-controllable mesoporous hydroxyapatite nanosphere, its preparation method, and its applications. The preparation method includes: S1, mixing an aqueous solution of pyridoxal phosphate with an aqueous solution of calcium chloride, adjusting the pH of the mixed solution to 7-11 to obtain a precursor solution; S2, placing the precursor solution in a microwave reactor for reaction to obtain mesoporous hydroxyapatite nanospheres. The preparation method provided by this invention is simple, the reaction conditions are easily controlled, and it is highly safe and reliable. It enables precise control of the morphology and particle size of the hydroxyapatite microspheres, and the prepared product exhibits excellent biocompatibility and structural regularity. It has good industrial application prospects in the field of biomedical implants such as bone tissue repair and is suitable for large-scale production.

The application belongs to the technical field of biomaterial preparation, and particularly relates to a composite drug delivery system based on macrophage membranes and a preparation method and application thereof. The composite drug delivery system takes a lipid nanoparticle as a drug targeting delivery carrier, coats quercetin through an active phagocytosis mode, coats a macrophage membrane on the surface of the lipid nanoparticle, and constructs a quercetin lipid nanoparticle coated with a macrophage membrane (MCM@QU@LNP). The composite drug delivery system provided by the application can promote drug enrichment in RA lesions, improve local drug exposure, realize precise drug delivery in the RA part, has good biological safety, and reduces system toxicity.

This application discloses a collagen microcapsule, its preparation method, and its application, belonging to the field of biomaterial preparation technology. The collagen microcapsule comprises: a collagen core material and a capsule material coating the surface of the collagen core material; the capsule material includes a mitochondrial membrane. By using a mitochondrial membrane as the encapsulating material, this application achieves precise coating of collagen, improving its biocompatibility and skin integration, and exhibiting good antioxidant properties, making it suitable for anti-aging applications. The prepared collagen microcapsules demonstrate good stability when dispersed in water at different temperatures, meeting the stability requirements for application in solution. When applied to skincare products, the beneficial elements in the collagen are better absorbed by the skin, improving the effectiveness of the skincare products.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a double-crosslinked AlgMA gel scaffold, its construction method, and its application. The construction method includes the following steps: S1, esterifying sodium alginate with methacrylic anhydride, dialyzing, and freeze-drying to obtain methacryloylated sodium alginate (AlgMA); S2, mixing the AlgMA obtained in S1 with a photoinitiator and performing photo-responsive crosslinking to obtain a photocrosslinked gel scaffold; S3, subjecting the photocrosslinked gel scaffold obtained in S2 to secondary ion-curing crosslinking to obtain a double-crosslinked AlgMA gel scaffold. This invention discloses a double-crosslinked AlgMA gel scaffold, its construction method, and its application. This double-crosslinked AlgMA gel scaffold exhibits high morphological fidelity, excellent mechanical properties, and controllable pore size distribution and porosity, meeting the requirements of biomimetic muscle tissue for scaffold mechanical adaptability.

This invention discloses a method and application for rapidly preparing humanized collagen using umbilical cord mesenchymal stem cell material, belonging to the field of biomaterial preparation technology. The key technical points of the method are as follows: S1, processing and passage-expanding umbilical cord tissue; S2, seeding the expanded umbilical cord mesenchymal stem cell suspension into a culture dish, then adding DMEM / F12 medium containing platelet lysis buffer for culture, followed by induction medium to obtain extracellular matrix; S3, enzymatically hydrolyzing the extracellular matrix, centrifuging the hydrolysate to obtain the supernatant, adjusting the pH, allowing it to stand, centrifuging again to obtain the precipitate, reconstituteing the precipitate, purifying it, centrifuging again, and then lyophilizing it; S4, forming the formulation to achieve efficient secretion and self-assembly of collagen, while utilizing extracellular matrix components (such as fibronectin and laminin) secreted by umbilical cord mesenchymal stem cells to assist collagen in forming an active product with a natural triple helix structure.

A photovoltaic apparatus prepared from a regenerative biomaterial, the apparatus comprising a carrier, one or more microalgae cells, a cathode, a permeable diaphragm, and an electrolyte. The carrier comprises a plurality of conductive sheets extending in a planar direction. The conductive sheets are electrically connected to each other. The microalgae cells are arranged on the carrier. The permeable diaphragm is arranged between the carrier and the cathode. The electrolyte is an algae culture medium and is in contact with the cathode and the microalgae cells. Each conductive sheet is provided with a first surface area for arranging the microalgae cells and in contact with the electrolyte. The cathode is provided with a second surface area in contact with the electrolyte. The microalgae cells capture sunlight in the electrolyte and grow a microalgae layer on the conductive sheets to serve as an anode.

The application provides a kind of recombinant collagen type III and its application, it is related to genetic engineering technical field.The application improves the yield of collagen by optimizing the nucleotide sequence of the gene of interest, optimizing the culture conditions of Pichia pastoris in multiple aspects, so that it is efficiently expressed in engineering bacteria.Recombinant engineering bacteria show strong potential in improving the yield of proteins with high economic value, and are suitable for large-scale industrial production.The recombinant collagen type III of the application has high expression, simple preparation process, is suitable for medical cosmetology, wound repair and other biological material preparation, and has broad application prospect.

The application discloses application of a viscoelastic biomaterial in the technical field of biological medicine, and aims to solve the problem of the influence mechanism of viscoelasticity on the metabolic state and pathway of nucleus pulposus cells in the prior art. The application comprises application of a viscoelastic double-network hydrogel of the viscoelastic biomaterial as a drug for promoting tissue repair and application of the viscoelastic biomaterial as a therapeutic drug for intervertebral disc degeneration; the application is suitable for development of a tissue repair drug, and a tissue repair drug is prepared from the viscoelastic biomaterial, so that a new route is provided for development of a tissue repair preparation.

The application belongs to the technical field of biomaterial preparation, and particularly relates to a polyvinyl alcohol biomimetic cornea and application thereof. The biomimetic cornea is prepared according to the following steps: after polyvinyl alcohol solution is cast into a film, pre-stretching, drying and water absorption are performed to obtain a single-layer hydrogel with fiber orientation; a plurality of the hydrogels with fiber orientation are stacked in a 90-degree orthogonal manner between adjacent layers, and polyvinyl alcohol solution is injected between the layers as an adhesive layer, and the obtained sample is subjected to multiple freeze-thaw cycles to form a multi-layer orthogonal structure hydrogel with a cornea-imitating structure. The application successfully prepares a polyvinyl alcohol biomimetic cornea with high strength, high transparency, good biocompatibility and excellent puncture resistance by pre-stretching induced orientation and multi-layer orthogonal structure design, and overcomes the contradiction between the mechanical properties and optical properties of the existing hydrogel materials, thereby providing an ideal candidate material for the clinical conversion of artificial corneas.

This invention provides a radioactive Cherenkov photoactivated intelligent therapeutic nanosensor, its preparation method, and its application, belonging to the field of biomaterials preparation technology. This invention achieves precise matching between the Cherenkov radiation spectrum and the absorption peak of a photosensitizer by constructing an intelligent therapeutic nanosensor, and establishes a dual-response release mechanism in the tumor microenvironment, realizing precise drug release under the synergistic triggering of acidic pH and overexpressed hydrogen sulfide. This invention utilizes the CR self-excitation effect to emit light in normal tissues, while simultaneously quenching fluorescence within the tumor where hydrogen sulfide is overexpressed, thereby achieving dual-locked Cherenkov radiation-mediated photodynamic therapy in the tumor microenvironment. The intelligent therapeutic nanosensor of this invention achieves the transformation from fluorescence emission in normal tissues to tumor-specific activated CR-PDT by forming deep hole trap energy levels, demonstrating excellent practicality.

This invention relates to the field of biomaterial preparation technology, specifically to a method for preparing polyhydroxyalkanoates (PHA) using fabric biotransformation. The method includes: (1) treating cotton-containing fabrics with alkali, followed by enzymatic hydrolysis to fully destroy the crystalline structure of cotton fibers in the fabrics; (2) sterilizing the hydrolysate obtained in step (1) and using it as a carbon source to inoculate PHA synthesizing bacteria for fermentation, allowing the PHA synthesizing bacteria to accumulate PHA during secondary metabolism. This invention treats cotton-containing fabrics with alkali and performs enzymatic hydrolysis to reduce the crystallinity of cellulose and destroy its dense structure, thereby improving the accessibility of the enzyme to the substrate. The resulting material is then directly used for the biosynthesis of PHA, thus realizing the high-value transformation of waste textiles from low-value waste to biodegradable polymer materials.