83 results about "Biomaterial preparation" patented technology

The invention discloses a preparation method of high-purity red date polysaccharide nano-selenium, and belongs to the field of biological material preparation. The preparation method of the high-purity red date polysaccharide nano-selenium comprises the following steps: adding water to dissolve red date polysaccharide to obtain a red date polysaccharide solution, then mixing the red date polysaccharide solution with a nano-selenium solution, reacting to obtain a mixed solution, and separating and purifying to obtain the red date polysaccharide nano-selenium. According to the preparation method disclosed by the invention, high-purity red date polysaccharide nano-selenium can be efficiently and stably prepared, the product has huge application potential in the fields of biomedicine, functional food and the like, and powerful technical support is provided for the development of related industries. Compared with a conventional process in the prior art, the preparation process for preparing the red date polysaccharide and the nano-selenium first and then compounding the red date polysaccharide and the nano-selenium to obtain the final product has obvious performance advantages in the aspects of product purity, storage stability and the like, and powerful technical support is provided for further application and development of the red date polysaccharide nano-selenium.

The invention belongs to the technical field of preparation of biological materials, and particularly discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application of the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The preparation method comprises the following steps: dissolving freeze-dried sea cucumber flower powder in deionized water, adjusting the pH value to be alkaline, heating and stirring, centrifuging, discarding the precipitate, adjusting the pH value to be acidic, centrifuging, collecting the precipitate, and freeze-drying to obtain sea cucumber flower protein powder; preparing a reaction solution, adding simulated gastric juice and simulated intestinal juice, stirring for reaction, inactivating protease, centrifuging, taking supernatant, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; the preparation method comprises the following steps: preparing a solution from sea cucumber, adding FeCl2. 4H2O and ascorbic acid, adjusting the pH value to be acidic, carrying out heating reaction, centrifuging, discarding the supernatant, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The invention discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application thereof. The carrier can promote absorption of an organism to iron, a relatively high iron ion release rate is maintained in an intestinal environment, and the in-vivo bioavailability of iron ions is improved.

The invention relates to the technical field of biological material preparation, and particularly discloses a method and a system for industrial continuous production of silk fibroin. In order to solve the problems of high energy consumption, heavy pollution, low efficiency, poor product stability and the like in the prior art, efficient removal of sericin is realized through a gradient degumming synergistic treatment technology (high-temperature and high-pressure alkali treatment and directional enzymolysis), and desalination and concentration are synchronously completed in combination with a membrane separation directional purification technology (a hollow fiber ultrafiltration system). The system integrates the gradient degumming module, the dissolution-filtration coupling module, the membrane separation module and the resource circulation subsystem, the technological parameters (degumming rate, viscosity and transmembrane pressure difference) are regulated and controlled in real time through the intelligent control center, the continuous production stability is ensured, the purity of the obtained silk fibroin solution is larger than or equal to 99%, and the standard of beauty makeup materials is met. The method and the system for industrial continuous production of silk fibroin have the advantages of high efficiency, low consumption and environmental protection, and are suitable for large-scale production.

The present invention discloses a method for preparing food-grade glassine paper from edible mushroom liquid fermentation mycelium and the food-grade glassine paper, which relates to a method for preparing glassine paper using natural biological materials. The preparation method of the food-grade glassine paper is as follows: Step 1: Preparation of the base paper of edible mushroom liquid fermentation mycelium; Step 2: Preparation of the surface paper of edible mushroom liquid fermentation mycelium; Step 3: Preparation of the food-grade glassine paper. The present invention prepares the food-grade glassine paper from edible mushroom liquid fermentation mycelium, which can replace the traditional papermaking method mainly using wood as the raw material, without adding plastics and chemical coating agents, and has the advantages of environmental protection, low carbon and degradability. It is a food-grade oil-proof, moisture-proof and semi-transparent glassine paper, and has broad application prospects in various fields such as food, medicine and express labels.

The invention belongs to the technical field of medical biological material preparation, and particularly relates to a sterile medical wound repair hydrogel dressing which takes dual-modified hyaluronic acid, polyvinyl alcohol, nano zinc, a humectant and a lubricant as main raw materials. The preparation method comprises the following steps: firstly, utilizing dual-modified hyaluronic acid as a core skeleton of a hydrogel system, and jointly constructing a double-layer network structure through free radical polymerization and crosslinking of boric acid ester bonds and carbon-carbon double bonds formed between phenylboronic acid groups and polyvinyl alcohol; meanwhile, polyvinyl alcohol and zinc ions form coordinate bonds which are interpenetrated in a network, so that the hydrogel dressing with a triple network structure is formed. The unique network structure can simulate an extracellular matrix microenvironment and is beneficial to promoting cell proliferation. In the using process, the hydrogel dressing can play a role in integrating multiple functions. Firstly, the hydrogel dressing can be attached to the surface of the skin to form a breathable physical barrier, meanwhile, invasion of bacteria is effectively isolated, secondly, moisture of the skin can be supplemented through the moisturizing performance of the hydrogel dressing, and due to the flexibility and elasticity of the hydrogel dressing, the use comfort of the hydrogel dressing is further improved, and painless dressing change is achieved. More importantly, long-acting release of the zinc ions can inhibit bacterial infection at the wound, promote wound repair and accelerate wound healing.

The invention provides recombinant III-type collagen and application thereof, and relates to the technical field of genetic engineering. According to the invention, the yield of the collagen is improved by optimizing the nucleotide sequence of the target gene and optimizing the culture conditions of pichia pastoris, so that the collagen is efficiently expressed in engineering bacteria. The recombinant engineering bacterium shows strong potential in the aspect of improving the yield of protein with high economic value, and is suitable for large-scale industrial production. The recombinant III-type collagen is high in expression quantity, simple in preparation process and suitable for preparation of biological materials for medical cosmetology, wound repair and the like, and has a wide application prospect.

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.

The present invention belongs to the field of biomaterial preparation, and discloses a thermosensitive hydrogel material and a preparation method thereof. The preparation method comprises: (1) adding chitosan to a dilute acetic acid solution, stirring and ultrasonically dispersing, to obtain solution A; (2) adding anhydrous citric acid to solution A, heating in a water bath, to obtain solution B; (3) dissolving gum arabic in deionized water, to obtain solution C; (4) adding solution C to solution B, blending and dispersing, and heating in a water bath, then pouring it into a 24-well plate, adding a certain amount of cross-linking agent, standing, to obtain a chitosan / anhydrous citric acid / gum arabic thermosensitive hydrogel material. The thermosensitive hydrogel preparation method adopted does not generate pollutants, is green and environmentally friendly, and is simple to operate; the prepared hydrogel material has chemical bonds formed by cross-linking inside, has good mechanical properties, has been greatly improved in terms of strength, biocompatibility, etc., has better mechanical strength and ductility, and is thermosensitive.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

The invention relates to the technical field of biological material preparation, in particular to a method for preparing carboxymethyl chitosan through irradiation treatment of chitin-enriched biomass and application. According to the method, chitin-enriched biomass is taken as a raw material and is sequentially subjected to pretreatment, irradiation pretreatment, decalcification treatment, degreasing and deproteinization, deacetylation and carboxymethylation; the irradiation absorbed dose of the irradiation pretreatment is greater than 0 and less than or equal to 1000kGy. According to the method, the structure porosity of chitin is improved through high-energy ray irradiation treatment, the permeation efficiency of subsequent reactions such as decalcification and deproteinization is remarkably improved, meanwhile, the dosage of chemical reagents is reduced, and the substitution degree and water solubility of the product are improved by further combining a multi-step synergistic chemical modification process such as freezing alkalization and gradient dialysis purification technologies; the molecular weight distribution is uniform. The high-purity carboxymethyl chitosan provides technical guarantee for development of novel medical materials.

This invention relates to the field of biomaterial preparation technology, and in particular to a morphology-controllable mesoporous hydroxyapatite nanosphere, its preparation method, and its applications. The preparation method includes: S1, mixing an aqueous solution of pyridoxal phosphate with an aqueous solution of calcium chloride, adjusting the pH of the mixed solution to 7-11 to obtain a precursor solution; S2, placing the precursor solution in a microwave reactor for reaction to obtain mesoporous hydroxyapatite nanospheres. The preparation method provided by this invention is simple, the reaction conditions are easily controlled, and it is highly safe and reliable. It enables precise control of the morphology and particle size of the hydroxyapatite microspheres, and the prepared product exhibits excellent biocompatibility and structural regularity. It has good industrial application prospects in the field of biomedical implants such as bone tissue repair and is suitable for large-scale production.

The invention belongs to the technical field of biological material preparation, and particularly relates to a copper-based metal organic framework nanoparticle as well as a preparation method and application thereof. The invention relates to a copper-based metal organic framework nano-particle which is obtained by carrying out hydrothermal reaction on a mixed solution, polyvinylpyrrolidone, 1, 3, 5-benzene tricarboxylic acid and a copper compound, the mixed solution is obtained by mixing deionized water, absolute ethyl alcohol and N, N-dimethylformamide; the ratio of the polyvinylpyrrolidone to the mixed solution is (70mg-90mg): 9mL; the ratio of the 1, 3, 5-benzene tricarboxylic acid to the copper compound to the mixed solution is (7g-14g): 7g: 1500mL. The particle size of the copper-based metal organic framework nano-particles is 167 nm, the loading rate of the copper-based metal organic framework nano-particles to adriamycin within 24 h reaches 78.3%, the copper-based metal organic framework nano-particles have the pH response drug release characteristic in a tumor microenvironment with the pH being 5.2, the cumulative release rate within 72 h reaches 54.75%, and the cumulative release rate is increased by 6.3 times compared with the physiological condition with the pH being 7.4. Complete cell internalization is realized within 6 hours, and acid-triggered drug release is promoted.

This invention discloses a bimetallic complex with whitening and anti-aging effects, its preparation method, and its application, belonging to the field of biomaterial preparation technology. The preparation method includes S1, dissolving an amino acid in water to obtain solution A; S2, dissolving a metal salt in ethanol to obtain solution B; S3, mixing solutions A and B evenly, and then reacting them through a solvothermal reaction to obtain solution C; S4, washing and drying solution C to obtain the final product. The amino acid in step S1 is at least one of glutamic acid and aspartic acid; the metal salt in step S2 is copper acetate and zinc acetate, copper acetate and manganese chloride, or copper acetate and cobalt nitrate; the solvothermal reaction conditions in step S3 are: temperature 60-110℃, reaction time 2-48h. The prepared bimetallic complex has both whitening and anti-aging effects, providing a new direction for the application of BioMOFs materials.

The invention discloses a method for improving mineralized collagen performance based on discrete increase of ion concentration, and belongs to the technical field of biological material preparation, and the method comprises the following steps: step 1, preparing a reaction stock solution; 2, preparing a dynamically mineralized collagen solution; and step 3, preparing the bracket with dynamically changed ion concentration. According to the method for improving the performance of the mineralized collagen based on discrete increase of the ion concentration, the nonlinear process of mineralization of natural bones is simulated by dynamically regulating and controlling the ion concentration, more inorganic minerals can be attached to the collagen, and the mineralization degree of the prepared collagen is higher on the micron scale; on the macroscopic scale, the mechanical property of the mineralized collagen is better, the mineral substances are more, and the technical support can be provided for preparing the multi-scale bionic bone matrix material.

Provided are a biological material for restoring ovarian functions, a preparation method therefor and the use thereof, relating to the technical field of biological materials. A large number of experiments verify that: it is found that a specific biological protein component for reversing ovarian functions in extracellular vesicles is the growth differentiation factor 9 (GDF-9), and the mechanism of action thereof for restoring the ovary is determined, and therefore extracellular vesicles (GOEs) that overexpress GDF-9 serve as an active substance, and by means of extraction and purification of said component, the purpose of targeted therapy is achieved.

The application discloses a PAM-AA-NHS-based multifunctional biomimetic polymer adhesive for wound repair and a preparation method thereof, and belongs to the field of biomaterial preparation and biomedical application. N-hydroxysuccinimide acrylate copolymerized polyacrylamide hydrogel is used as a substrate and as a main chain network, and functionalized materials tannic acid and phenolic hydroxyl chitosan containing o-diphenol groups are introduced, so that effective adhesion can be formed at a biological tissue interface, and cross-linking can be generated with the main chain network through hydrogen bond action to form a secondary network. In addition, aldehyde group-rich oxidized dextran side chains are introduced, which can form Schiff base cross-linking with the main chain network and amino groups in the biological tissue interface to form long-acting adhesion performance. The adhesive has good tissue adhesion, biocompatibility, antibacterial activity and wound healing promotion capacity, can replace sutures, has a positive effect on the formation of new tissues and tissue healing, and is expected to be applied in clinical treatment of wound surfaces.

The invention discloses porous biological ceramic as well as a preparation method and application thereof, and belongs to the technical field of biological material preparation. The preparation method comprises the following steps: dropwise adding a phosphorus-containing compound solution into a calcium-containing compound solution under the condition that the pH value is 6-12, reacting to generate a biological ceramic precursor, centrifuging, drying, calcining, grinding and sieving the precursor to obtain biological ceramic powder, and then mixing the powder with a dispersing agent, a binder and water to prepare biological ceramic slurry; mixing the slurry with a pore-forming agent, and drying to obtain a biological ceramic blank; and finally, shaping and sintering the blank body to obtain the porous biological ceramic. According to the method, regulation and control of the pH value of a reaction system are taken as a main means, and the calcination temperature of the precursor is controlled, so that continuous, accurate and repeatable control on the proportion of two phases of hydroxyapatite and beta-tricalcium phosphate in the biological ceramic can be realized.

The present invention relates to a crosslinkable polymer composition comprising at least one calcium phosphate, at least one biocompatible and biodegradable organic polymer or one of its precursors, and Zn-ZnO core-shell particles, a biomaterial obtainable by photopolymerization of such a crosslinkable polymer composition, methods for preparing such a composition and such a biomaterial, and the use of such a biomaterial for various applications in the health sector.

The present invention discloses an injectable dual - biomimetic bone adhesive for promoting fracture healing and its preparation method, belonging to the field of biomaterial preparation. Inspired by the underwater adhesion mechanisms of marine mussels and barnacles, it uses biomimetic marine mussel substances (such as tannic acid), biomimetic marine barnacle substances (such as sodium alginate), silk fibroin, calcium chloride, and magnesium particles to form a dual - biomimetic bone adhesive with wet adhesion. Based on the hydrogen bonds, metal - phenol coordination bonds, electrostatic interactions, and β β - sheet protein folding conformations formed among the components, the obtained dual - biomimetic bone adhesive not only has good biocompatibility, excellent wet adhesion, and mechanical properties matching bone tissue strength, can be used for fracture bone fixation and repair, but also has the ability of in - situ gelation and can be directly injected at the injury site. The raw materials used in the adhesive prepared by the present invention are safe and widely sourced. Therefore, it is expected to achieve potential clinical application value in the fixation and repair of bone injuries.

The invention belongs to the field of biological material preparation, and particularly relates to degradable drug-loading hydrogel and a preparation method thereof.Acrylate groups are introduced into 6s-PLA, 6s-PLA-HA is synthesized, a hydrogel material is synthesized through a photopolymerization method, then cefprozil serves as a model drug, and drug loading is conducted on the hydrogel through the supercritical impregnation technology. The composite material is self-prepared six-arm star-shaped polylactic acid (6s-PLA), the 6s-PLA is terminated by using acryloyl chloride to synthesize 6s-PLA-HA, PLA-PEG hydrogel is prepared by using a photopolymerization method, and a hydrogel material loaded with cefprozil is prepared by using a supercritical carbon dioxide impregnation technology. The material has the advantages of being excellent in mechanical property, non-toxic, good in biological safety, non-irritant, easy to process and form, degradable, anti-inflammatory and the like, and is expected to be widely applied to the field of biomedical materials.

The invention discloses an ROS (reactive oxygen species) sensitive hydrogel composite polycaprolactone stent as well as a preparation method and application thereof, and belongs to the technical field of biological material preparation. The preparation method comprises the following steps: reacting 4-(bromomethyl) phenylboronic acid and TMPDA in a solvent, and collecting precipitate to prepare a cross-linking agent; blending and cross-linking the PVA solution and the cross-linking agent solution to prepare PVA / TPA hydrogel; taking a mixed solution containing PCL and nHA as printing slurry, and obtaining a PCL / nHA stent through 3D printing; and pouring the PVA / TPA hydrogel into the PCL / nHA scaffold, and freeze-drying to obtain the hydrogel. The ROS sensitive hydrogel composite polycaprolactone scaffold prepared by the invention is a composite material with soft and hard combination and communicated large and small pores, has excellent biocompatibility, ROS removal performance, anti-inflammatory performance and osteogenic differentiation performance, can enhance the bone regeneration capacity in an inflammatory microenvironment, and has wide application prospects.

The invention belongs to the technical field of biological material preparation, and provides genipin cross-linked black bean peptidyl tea polyphenol sustained-release gel balls and a preparation method thereof. The preparation method comprises the following steps: dissolving black bean protein in water, carrying out microwave treatment, heating and incubating, adding protease for enzymolysis modification, and carrying out enzyme deactivation and precipitate removal to obtain a black bean peptide solution; the preparation method comprises the following steps: uniformly mixing black soybean peptide with a sodium alginate solution, adding tea polyphenol, uniformly stirring and mixing, adding a genipin aqueous solution, carrying out constant-temperature water bath, and dropwise adding the obtained solution into a calcium chloride solution to form gel balls; the prepared genipin cross-linked black bean peptidyl tea polyphenol sustained-release gel ball has a compact and stable wrinkled three-dimensional network structure and is high in biocompatibility and dispersity, and the stability of tea polyphenol is remarkably improved; the pH-responsive tea polyphenol slow release performance is achieved, accurate slow release of the tea polyphenol in intestinal juice is achieved, and the bioavailability of the tea polyphenol is improved.

The application belongs to the technical field of biomaterial preparation, and particularly relates to a composite drug delivery system based on macrophage membranes and a preparation method and application thereof. The composite drug delivery system takes a lipid nanoparticle as a drug targeting delivery carrier, coats quercetin through an active phagocytosis mode, coats a macrophage membrane on the surface of the lipid nanoparticle, and constructs a quercetin lipid nanoparticle coated with a macrophage membrane (MCM@QU@LNP). The composite drug delivery system provided by the application can promote drug enrichment in RA lesions, improve local drug exposure, realize precise drug delivery in the RA part, has good biological safety, and reduces system toxicity.

The present application belongs to the field of biological medicine, and relates to an in-situ spraying particle-gel composite as well as a preparation method and application thereof. The present application prepares a drug-loaded particle-gel composite material, which can be rapidly gelled in-situ in the form of spraying. In a wound infection model of diabetic rats, the composite material exhibits good photothermal activation TRPV1 channel-mediated local analgesia, as well as anti-inflammatory, antibacterial, antioxidant, hemostatic and wound healing-promoting multifunctional therapeutic effects. Through in-vitro and in-vivo material characterization, as well as histopathological examination of animal experiments, it is confirmed that the composite gel material has good biological safety and degradability, and is a local anesthetic biomaterial preparation which is expected to be clinically transformed for chronic wound analgesia and healing-promoting therapeutic effects.

The application discloses a preparation method and system of a tendon / ligament-bone junction special trabecular zone biomechanical bidirectional biomimetic bone biological structure, and belongs to the technical field of bone biological material preparation, and comprises the following steps: extracting morphological information of trabeculae and mechanical parameters of a tendon / ligament-bone junction based on a target region of a human specimen slice; determining a biomimetic structure based on the morphological information of the trabeculae; determining a biological material for preparing the biomimetic structure based on the mechanical parameters of the tendon / ligament-bone junction; preparing the biomimetic bone biological structure based on the biomimetic structure and the bone biological material; and performing modification treatment on a surface of the biomimetic bone biological structure to obtain a final biomimetic bone biological structure. The application combines the construction information of the trabeculae of the tendon / ligament-bone junction with 3D printing technology, realizes the structure and material collaborative biomimetic of an implant when the tendon / ligament-bone junction is reconstructed, and provides important technical support for researching and developing a bone biological structure with better biocompatibility for the tendon / ligament-bone junction.

The application provides a preparation method and application of tremella polysaccharide intelligent hydrogel for inhibiting ferroptosis, and relates to the technical field of biomaterial preparation, wherein the tremella polysaccharide intelligent hydrogel for inhibiting ferroptosis is prepared from methacrylated tremella polysaccharide TrepMA, oxidized tremella polysaccharide O-Trep and ketone thioalcohol diamine TK-NH2. The tremella polysaccharide intelligent hydrogel prepared by the method has a three-dimensional network structure, good mechanical properties and can load growth factors or active molecules to accelerate bone regeneration. In addition, the tremella polysaccharide intelligent hydrogel can inhibit ferroptosis and promote bone defect healing with osteoporosis. The tremella polysaccharide intelligent hydrogel prepared by the method has multiple biological activities, can not only provide certain support for bone regeneration, but also can inhibit ferroptosis, improve local osteoporosis, has antioxidant / anti-inflammatory and osteogenic differentiation induction effects through excellent biocompatibility, biological activity and drug controlled release effect, and can effectively promote bone tissue regeneration.

The invention discloses a method for promoting coupling of DNA oligonucleotides and macromolecules by using ice-phase confinement and application of the method, and belongs to the technical field of biological material preparation. The method comprises the following steps: freezing single-stranded DNA containing a terminal reaction group and a buffer solution of macromolecules to be coupled under the condition of-20 DEG C, and enriching solutes in a micro water sac area between ice crystals, so that local concentration and reaction site accessibility are remarkably improved, and various coupling chemistry (SPAAC, CuAAC, biotin-streptavidin and the like) can be quickly and freely completed by a template at low temperature. The method is high in universality, mild in condition and low in reagent consumption, high-efficiency construction of long-chain DNA, 3 '-3' / 5 '-5' inverted connection and internal site modification can be achieved, the coupling efficiency of antibody-DNA and BSA-DNA can be remarkably improved under the condition that protein conformation and functions are maintained, a valence-controllable nucleic acid-protein composite structure is achieved, and the method is suitable for industrial production. The method is suitable for the fields of nucleic acid probe development, protein engineering, nano material construction, drug delivery and the like.

The present application belongs to the technical field of biomaterial preparation, and particularly relates to a preparation method of a self-oxygen-supplying hydrogel cell scaffold, the obtained product and application. The present application realizes stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres. The method is realized through the following steps: compounds P-MA, P-Cys, ColMA and PEG-CaO2 are added to a PBS solution, a photoinitiator is added, and a'self-oxygen-supplying' composite hydrogel is obtained by irradiation with ultraviolet light of a wavelength of 365 nm. The present application provides a composite hydrogel loaded with oxygen-producing nanoparticles for 3D cell sphere culture and a new method for continuous release of O2 in a slightly acidic environment, aiming at the problems of intermediate cell death caused by hypoxia in the process of 3D culture of existing cell spheres, unclear matrigel composition, high price and the like. The present application solves the problem of internal hypoxia in the process of cell sphere culture, and clearly defines the gel composition for studying the interaction between other components and cells. In addition, the self-oxygen-supplying hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

The present invention belongs to the field of biocompatible material preparation, and specifically discloses a preparation method and application of an acryloylphenylalanine-N-vinylpyrrolidone chiral hydrogel film. Based on polyethylene glycol diacrylate as a crosslinking agent, the present invention obtains a chiral copolymer gel film based on acryloylphenylalanine and N-vinylpyrrolidone by ultraviolet light-induced copolymerization of monomer acryloylphenylalanine and monomer N-vinylpyrrolidone. The present invention introduces chirality into the gel film through a gel preparation method to obtain a chiral gel interface suitable for cell culture. The chiral gel interface has better advantages similar to the extracellular matrix (ECM), and the chiral gel surface containing a large amount of water can regulate cell adhesion and proliferation.

The present invention relates to a programmable hydrogel, a preparation method thereof and its application in the preparation of a medical self-expanding stent, and belongs to the field of biomedical material technology. In order to solve the problem that existing programmable hydrogels cannot meet the requirement of mild induced stimulation of biomaterials, the present invention provides a programmable hydrogel, wherein formaldehyde-modified xylan is mixed with gelatin to prepare a gel, a shaped product is prepared by 3D printing or mold shaping, and the product is immersed in a salt solution for salting out to obtain a programmable hydrogel. The present invention utilizes the Hofmeister effect to make the editable hydrogel exhibit ion stimulation response and shape memory characteristics, and can achieve self-expansion under mild stimulation of low-concentration ion solution or distilled water. The editable hydrogel provided by the present invention meets the requirements of simple biomaterial preparation, suitable mechanical strength, mild induced stimulation, non-toxicity, and good biocompatibility, and has great application prospects in the preparation of self-expanding artificial stents.