62 results about "Biomaterial preparation" patented technology

The invention belongs to the technical field of preparation of biological materials, and particularly discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application of the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The preparation method comprises the following steps: dissolving freeze-dried sea cucumber flower powder in deionized water, adjusting the pH value to be alkaline, heating and stirring, centrifuging, discarding the precipitate, adjusting the pH value to be acidic, centrifuging, collecting the precipitate, and freeze-drying to obtain sea cucumber flower protein powder; preparing a reaction solution, adding simulated gastric juice and simulated intestinal juice, stirring for reaction, inactivating protease, centrifuging, taking supernatant, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; the preparation method comprises the following steps: preparing a solution from sea cucumber, adding FeCl2. 4H2O and ascorbic acid, adjusting the pH value to be acidic, carrying out heating reaction, centrifuging, discarding the supernatant, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The invention discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application thereof. The carrier can promote absorption of an organism to iron, a relatively high iron ion release rate is maintained in an intestinal environment, and the in-vivo bioavailability of iron ions is improved.

The invention relates to the technical field of biological material preparation, and particularly discloses a method and a system for industrial continuous production of silk fibroin. In order to solve the problems of high energy consumption, heavy pollution, low efficiency, poor product stability and the like in the prior art, efficient removal of sericin is realized through a gradient degumming synergistic treatment technology (high-temperature and high-pressure alkali treatment and directional enzymolysis), and desalination and concentration are synchronously completed in combination with a membrane separation directional purification technology (a hollow fiber ultrafiltration system). The system integrates the gradient degumming module, the dissolution-filtration coupling module, the membrane separation module and the resource circulation subsystem, the technological parameters (degumming rate, viscosity and transmembrane pressure difference) are regulated and controlled in real time through the intelligent control center, the continuous production stability is ensured, the purity of the obtained silk fibroin solution is larger than or equal to 99%, and the standard of beauty makeup materials is met. The method and the system for industrial continuous production of silk fibroin have the advantages of high efficiency, low consumption and environmental protection, and are suitable for large-scale production.

The invention provides recombinant III-type collagen and application thereof, and relates to the technical field of genetic engineering. According to the invention, the yield of the collagen is improved by optimizing the nucleotide sequence of the target gene and optimizing the culture conditions of pichia pastoris, so that the collagen is efficiently expressed in engineering bacteria. The recombinant engineering bacterium shows strong potential in the aspect of improving the yield of protein with high economic value, and is suitable for large-scale industrial production. The recombinant III-type collagen is high in expression quantity, simple in preparation process and suitable for preparation of biological materials for medical cosmetology, wound repair and the like, and has a wide application prospect.

This invention belongs to the field of biomaterial preparation technology, specifically disclosing a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. The preparation method includes the following steps: S1, dissolving β-alanyl-L-histidine in ultrapure water and stirring until homogeneous to obtain solution A; S2, dissolving heme chloride in dimethyl sulfoxide and stirring until homogeneous to obtain solution B; S3, mixing solution A and solution B until homogeneous, adjusting the pH, heating in a water bath, incubating at room temperature, centrifuging, discarding the precipitate, and retaining the supernatant; S4, lyophilizing and grinding the supernatant obtained in S3 to obtain the self-assembled supramolecular peptide nanozyme material. This invention discloses a self-assembled supramolecular peptide nanozyme material, its preparation method, and its application. This supramolecular peptide nanozyme, when applied to food quality and safety testing, can achieve visual and sensitive colorimetric detection of biogenic amines in spoiled food.

This invention relates to the field of biomaterial preparation technology, and in particular to a morphology-controllable mesoporous hydroxyapatite nanosphere, its preparation method, and its applications. The preparation method includes: S1, mixing an aqueous solution of pyridoxal phosphate with an aqueous solution of calcium chloride, adjusting the pH of the mixed solution to 7-11 to obtain a precursor solution; S2, placing the precursor solution in a microwave reactor for reaction to obtain mesoporous hydroxyapatite nanospheres. The preparation method provided by this invention is simple, the reaction conditions are easily controlled, and it is highly safe and reliable. It enables precise control of the morphology and particle size of the hydroxyapatite microspheres, and the prepared product exhibits excellent biocompatibility and structural regularity. It has good industrial application prospects in the field of biomedical implants such as bone tissue repair and is suitable for large-scale production.

The invention belongs to the technical field of biological material preparation, and particularly relates to a copper-based metal organic framework nanoparticle as well as a preparation method and application thereof. The invention relates to a copper-based metal organic framework nano-particle which is obtained by carrying out hydrothermal reaction on a mixed solution, polyvinylpyrrolidone, 1, 3, 5-benzene tricarboxylic acid and a copper compound, the mixed solution is obtained by mixing deionized water, absolute ethyl alcohol and N, N-dimethylformamide; the ratio of the polyvinylpyrrolidone to the mixed solution is (70mg-90mg): 9mL; the ratio of the 1, 3, 5-benzene tricarboxylic acid to the copper compound to the mixed solution is (7g-14g): 7g: 1500mL. The particle size of the copper-based metal organic framework nano-particles is 167 nm, the loading rate of the copper-based metal organic framework nano-particles to adriamycin within 24 h reaches 78.3%, the copper-based metal organic framework nano-particles have the pH response drug release characteristic in a tumor microenvironment with the pH being 5.2, the cumulative release rate within 72 h reaches 54.75%, and the cumulative release rate is increased by 6.3 times compared with the physiological condition with the pH being 7.4. Complete cell internalization is realized within 6 hours, and acid-triggered drug release is promoted.

This invention discloses a bimetallic complex with whitening and anti-aging effects, its preparation method, and its application, belonging to the field of biomaterial preparation technology. The preparation method includes S1, dissolving an amino acid in water to obtain solution A; S2, dissolving a metal salt in ethanol to obtain solution B; S3, mixing solutions A and B evenly, and then reacting them through a solvothermal reaction to obtain solution C; S4, washing and drying solution C to obtain the final product. The amino acid in step S1 is at least one of glutamic acid and aspartic acid; the metal salt in step S2 is copper acetate and zinc acetate, copper acetate and manganese chloride, or copper acetate and cobalt nitrate; the solvothermal reaction conditions in step S3 are: temperature 60-110℃, reaction time 2-48h. The prepared bimetallic complex has both whitening and anti-aging effects, providing a new direction for the application of BioMOFs materials.

The invention discloses a method for improving mineralized collagen performance based on discrete increase of ion concentration, and belongs to the technical field of biological material preparation, and the method comprises the following steps: step 1, preparing a reaction stock solution; 2, preparing a dynamically mineralized collagen solution; and step 3, preparing the bracket with dynamically changed ion concentration. According to the method for improving the performance of the mineralized collagen based on discrete increase of the ion concentration, the nonlinear process of mineralization of natural bones is simulated by dynamically regulating and controlling the ion concentration, more inorganic minerals can be attached to the collagen, and the mineralization degree of the prepared collagen is higher on the micron scale; on the macroscopic scale, the mechanical property of the mineralized collagen is better, the mineral substances are more, and the technical support can be provided for preparing the multi-scale bionic bone matrix material.

Provided are a biological material for restoring ovarian functions, a preparation method therefor and the use thereof, relating to the technical field of biological materials. A large number of experiments verify that: it is found that a specific biological protein component for reversing ovarian functions in extracellular vesicles is the growth differentiation factor 9 (GDF-9), and the mechanism of action thereof for restoring the ovary is determined, and therefore extracellular vesicles (GOEs) that overexpress GDF-9 serve as an active substance, and by means of extraction and purification of said component, the purpose of targeted therapy is achieved.

The application discloses a PAM-AA-NHS-based multifunctional biomimetic polymer adhesive for wound repair and a preparation method thereof, and belongs to the field of biomaterial preparation and biomedical application. N-hydroxysuccinimide acrylate copolymerized polyacrylamide hydrogel is used as a substrate and as a main chain network, and functionalized materials tannic acid and phenolic hydroxyl chitosan containing o-diphenol groups are introduced, so that effective adhesion can be formed at a biological tissue interface, and cross-linking can be generated with the main chain network through hydrogen bond action to form a secondary network. In addition, aldehyde group-rich oxidized dextran side chains are introduced, which can form Schiff base cross-linking with the main chain network and amino groups in the biological tissue interface to form long-acting adhesion performance. The adhesive has good tissue adhesion, biocompatibility, antibacterial activity and wound healing promotion capacity, can replace sutures, has a positive effect on the formation of new tissues and tissue healing, and is expected to be applied in clinical treatment of wound surfaces.

The invention discloses porous biological ceramic as well as a preparation method and application thereof, and belongs to the technical field of biological material preparation. The preparation method comprises the following steps: dropwise adding a phosphorus-containing compound solution into a calcium-containing compound solution under the condition that the pH value is 6-12, reacting to generate a biological ceramic precursor, centrifuging, drying, calcining, grinding and sieving the precursor to obtain biological ceramic powder, and then mixing the powder with a dispersing agent, a binder and water to prepare biological ceramic slurry; mixing the slurry with a pore-forming agent, and drying to obtain a biological ceramic blank; and finally, shaping and sintering the blank body to obtain the porous biological ceramic. According to the method, regulation and control of the pH value of a reaction system are taken as a main means, and the calcination temperature of the precursor is controlled, so that continuous, accurate and repeatable control on the proportion of two phases of hydroxyapatite and beta-tricalcium phosphate in the biological ceramic can be realized.

The present invention relates to a crosslinkable polymer composition comprising at least one calcium phosphate, at least one biocompatible and biodegradable organic polymer or one of its precursors, and Zn-ZnO core-shell particles, a biomaterial obtainable by photopolymerization of such a crosslinkable polymer composition, methods for preparing such a composition and such a biomaterial, and the use of such a biomaterial for various applications in the health sector.

The invention belongs to the field of biological material preparation, and particularly relates to degradable drug-loading hydrogel and a preparation method thereof.Acrylate groups are introduced into 6s-PLA, 6s-PLA-HA is synthesized, a hydrogel material is synthesized through a photopolymerization method, then cefprozil serves as a model drug, and drug loading is conducted on the hydrogel through the supercritical impregnation technology. The composite material is self-prepared six-arm star-shaped polylactic acid (6s-PLA), the 6s-PLA is terminated by using acryloyl chloride to synthesize 6s-PLA-HA, PLA-PEG hydrogel is prepared by using a photopolymerization method, and a hydrogel material loaded with cefprozil is prepared by using a supercritical carbon dioxide impregnation technology. The material has the advantages of being excellent in mechanical property, non-toxic, good in biological safety, non-irritant, easy to process and form, degradable, anti-inflammatory and the like, and is expected to be widely applied to the field of biomedical materials.

The application belongs to the technical field of biomaterial preparation, and particularly relates to a composite drug delivery system based on macrophage membranes and a preparation method and application thereof. The composite drug delivery system takes a lipid nanoparticle as a drug targeting delivery carrier, coats quercetin through an active phagocytosis mode, coats a macrophage membrane on the surface of the lipid nanoparticle, and constructs a quercetin lipid nanoparticle coated with a macrophage membrane (MCM@QU@LNP). The composite drug delivery system provided by the application can promote drug enrichment in RA lesions, improve local drug exposure, realize precise drug delivery in the RA part, has good biological safety, and reduces system toxicity.

The present application belongs to the field of biological medicine, and relates to an in-situ spraying particle-gel composite as well as a preparation method and application thereof. The present application prepares a drug-loaded particle-gel composite material, which can be rapidly gelled in-situ in the form of spraying. In a wound infection model of diabetic rats, the composite material exhibits good photothermal activation TRPV1 channel-mediated local analgesia, as well as anti-inflammatory, antibacterial, antioxidant, hemostatic and wound healing-promoting multifunctional therapeutic effects. Through in-vitro and in-vivo material characterization, as well as histopathological examination of animal experiments, it is confirmed that the composite gel material has good biological safety and degradability, and is a local anesthetic biomaterial preparation which is expected to be clinically transformed for chronic wound analgesia and healing-promoting therapeutic effects.

The application discloses a preparation method and system of a tendon / ligament-bone junction special trabecular zone biomechanical bidirectional biomimetic bone biological structure, and belongs to the technical field of bone biological material preparation, and comprises the following steps: extracting morphological information of trabeculae and mechanical parameters of a tendon / ligament-bone junction based on a target region of a human specimen slice; determining a biomimetic structure based on the morphological information of the trabeculae; determining a biological material for preparing the biomimetic structure based on the mechanical parameters of the tendon / ligament-bone junction; preparing the biomimetic bone biological structure based on the biomimetic structure and the bone biological material; and performing modification treatment on a surface of the biomimetic bone biological structure to obtain a final biomimetic bone biological structure. The application combines the construction information of the trabeculae of the tendon / ligament-bone junction with 3D printing technology, realizes the structure and material collaborative biomimetic of an implant when the tendon / ligament-bone junction is reconstructed, and provides important technical support for researching and developing a bone biological structure with better biocompatibility for the tendon / ligament-bone junction.

The application provides a preparation method and application of tremella polysaccharide intelligent hydrogel for inhibiting ferroptosis, and relates to the technical field of biomaterial preparation, wherein the tremella polysaccharide intelligent hydrogel for inhibiting ferroptosis is prepared from methacrylated tremella polysaccharide TrepMA, oxidized tremella polysaccharide O-Trep and ketone thioalcohol diamine TK-NH2. The tremella polysaccharide intelligent hydrogel prepared by the method has a three-dimensional network structure, good mechanical properties and can load growth factors or active molecules to accelerate bone regeneration. In addition, the tremella polysaccharide intelligent hydrogel can inhibit ferroptosis and promote bone defect healing with osteoporosis. The tremella polysaccharide intelligent hydrogel prepared by the method has multiple biological activities, can not only provide certain support for bone regeneration, but also can inhibit ferroptosis, improve local osteoporosis, has antioxidant / anti-inflammatory and osteogenic differentiation induction effects through excellent biocompatibility, biological activity and drug controlled release effect, and can effectively promote bone tissue regeneration.

The invention discloses a method for promoting coupling of DNA oligonucleotides and macromolecules by using ice-phase confinement and application of the method, and belongs to the technical field of biological material preparation. The method comprises the following steps: freezing single-stranded DNA containing a terminal reaction group and a buffer solution of macromolecules to be coupled under the condition of-20 DEG C, and enriching solutes in a micro water sac area between ice crystals, so that local concentration and reaction site accessibility are remarkably improved, and various coupling chemistry (SPAAC, CuAAC, biotin-streptavidin and the like) can be quickly and freely completed by a template at low temperature. The method is high in universality, mild in condition and low in reagent consumption, high-efficiency construction of long-chain DNA, 3 '-3' / 5 '-5' inverted connection and internal site modification can be achieved, the coupling efficiency of antibody-DNA and BSA-DNA can be remarkably improved under the condition that protein conformation and functions are maintained, a valence-controllable nucleic acid-protein composite structure is achieved, and the method is suitable for industrial production. The method is suitable for the fields of nucleic acid probe development, protein engineering, nano material construction, drug delivery and the like.

The present application belongs to the technical field of biomaterial preparation, and particularly relates to a preparation method of a self-oxygen-supplying hydrogel cell scaffold, the obtained product and application. The present application realizes stable and continuous release of oxygen and three-dimensional (3D) culture of neural stem cell spheres. The method is realized through the following steps: compounds P-MA, P-Cys, ColMA and PEG-CaO2 are added to a PBS solution, a photoinitiator is added, and a'self-oxygen-supplying' composite hydrogel is obtained by irradiation with ultraviolet light of a wavelength of 365 nm. The present application provides a composite hydrogel loaded with oxygen-producing nanoparticles for 3D cell sphere culture and a new method for continuous release of O2 in a slightly acidic environment, aiming at the problems of intermediate cell death caused by hypoxia in the process of 3D culture of existing cell spheres, unclear matrigel composition, high price and the like. The present application solves the problem of internal hypoxia in the process of cell sphere culture, and clearly defines the gel composition for studying the interaction between other components and cells. In addition, the self-oxygen-supplying hydrogel material prepared by the technology is expected to be applied to 3D culture of other cell spheres or organoid spheres.

The present invention relates to a programmable hydrogel, a preparation method thereof and its application in the preparation of a medical self-expanding stent, and belongs to the field of biomedical material technology. In order to solve the problem that existing programmable hydrogels cannot meet the requirement of mild induced stimulation of biomaterials, the present invention provides a programmable hydrogel, wherein formaldehyde-modified xylan is mixed with gelatin to prepare a gel, a shaped product is prepared by 3D printing or mold shaping, and the product is immersed in a salt solution for salting out to obtain a programmable hydrogel. The present invention utilizes the Hofmeister effect to make the editable hydrogel exhibit ion stimulation response and shape memory characteristics, and can achieve self-expansion under mild stimulation of low-concentration ion solution or distilled water. The editable hydrogel provided by the present invention meets the requirements of simple biomaterial preparation, suitable mechanical strength, mild induced stimulation, non-toxicity, and good biocompatibility, and has great application prospects in the preparation of self-expanding artificial stents.

The invention belongs to the technical field of preparation of bone repair biological materials, and particularly relates to a preparation method of large-size composite bone repair particles, which comprises the following steps: S1, proportionally mixing polymer powder, inorganic biological ceramic powder and a binder; s2, adding the mixed material obtained in the step S1 into rotary granulation equipment; s3, obtaining wet composite particles by using rotary granulation equipment; s4, curing the composite particles prepared in the step S3 at a high temperature for a period of time; and S5, screening the cured particles prepared in the step S4 to obtain the composite particles with the required size. The preparation method comprises the following steps: preparing wet particles from polymer powder and inorganic biological ceramic powder by using a binder, carrying out high-temperature treatment, and finally screening to obtain large-size organic / inorganic composite particles; compared with the prior art, the method has the advantages that the obtained particles are large in size and low in brittleness, the inorganic proportion can reach a high level, and the method is stable and controllable, can be effectively applied to bone filling and repairing, and has a good application prospect.

The invention discloses a bone biological structure preparation method and system for mechanical biology bidirectional bionic of a special bone trabecula area at a tendon / ligament bone joint, and belongs to the technical field of bone biological material preparation, and the method comprises the following steps: extracting morphological information of a bone trabecula and mechanical parameters of the tendon / ligament bone joint based on a human body specimen slice of a target area; determining a bionic structure based on the morphological information of the bone trabecula; determining a biological material for preparing a bionic structure based on mechanical parameters of the tendon / ligament bone joint; preparing a bionic bone biological structure based on the bionic structure and the bone biological material; and carrying out modification treatment on the surface of the bionic bone biological structure to obtain the final bionic bone biological structure. The construction information of the bone trabecula at the tendon / ligament bone joint is combined with the 3D printing technology, so that when the tendon / ligament bone joint is reconstructed, the structure and material of the implant are synergistically bionic, and important technical support is provided for research and development of a bone biological structure with better biocompatibility at the tendon / ligament bone joint.

The invention belongs to the technical field of biological material preparation, and particularly discloses a mackerel tetrapeptide-calcium nano-composite as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing mackerel flesh homogenate with an organic degreasing fluid, stirring in a constant-temperature water bath, carrying out suction filtration, air-drying and powdering to obtain mackerel protein powder; preparing a reaction solution, heating, cooling, preserving heat, carrying out alkaline enzymolysis, inactivating protease, centrifuging, taking supernatant, and freeze-drying to obtain lyophilized powder of the mackerel protein hydrolysate; identifying a polypeptide sequence, and screening a mackerel tetrapeptide amino acid sequence; the mackerel tetrapeptide is obtained by solid-phase synthesis of macromolecular resin and amino acid. The preparation method comprises the following steps: dissolving mackerel tetrapeptide in ultrapure water, adding a CaCl2 solution, heating under an alkaline condition, adding absolute ethyl alcohol, centrifuging and freeze-drying to obtain the mackerel tetrapeptide-calcium nanocomposite. The invention discloses a mackerel tetrapeptide-calcium nano-composite as well as a preparation method and application thereof. The mackerel tetrapeptide-calcium nano-composite has a self-assembly capability and a calcium absorption promoting effect.

The invention relates to the technical field of preparation of photocatalysts from biological materials, and particularly discloses a method for preparing a photocatalyst from a biological material, which comprises the following steps: step 1, selecting biomass rich in carbon, and performing pretreatment in manners of drying, crushing, cleaning, hydrolyzing and the like; 2, carbonizing the pretreated biomass; 3, preparing a photocatalyst precursor; step 4, mixing the biomass charcoal powder with the photocatalyst precursor solution, and doping the carbon element into the photocatalyst; 5, performing high-temperature calcination on the mixture; and step 6, cooling, cleaning and drying the calcined sample to obtain the carbon-doped photocatalyst, wherein the biomass raw material is placed in a drying oven at 60-80 DEG C to be dried for 2-3 hours during drying in the step 1. According to the preparation method for preparing the photocatalyst by using the biological material, the COD (Chemical Oxygen Demand) of high-concentration organic wastewater can be reduced from 1000 mg / L to 200 mg / L or below under ultraviolet light.

A system is provided.SOLUTION: A system comprising: means for measuring the constituents of a sample collected from the ocean; means for performing detailed constituent analysis using a production AI; means for performing evaluation based on parameters of constituent amount, sustainability, and production cost; means for selecting an optimal bio-raw material; and means for experimentally producing bio-plastics based on the selected bio-raw material and collecting the results thereof.SELECTED DRAWING: Figure 1

The present invention relates to polysaccharide-based polymeric crosslinkers, polysaccharide-based biomaterials, preparation methods, and uses. In the present invention, the polysaccharide-based polymeric crosslinker is a polysaccharide-based polymeric crosslinker modified with ortho-phthalaldehyde groups, which does not rely on a synthetic hydrophilic polymer backbone or complex chemical synthesis processes. The polysaccharide-based polymeric crosslinker provided by the present invention, together with water-soluble small molecules or polymers containing one or more groups selected from the group consisting of primary amine, hydrazine, hydrazide, hydroxylamine, and mercapto groups, forms a two-component polysaccharide-based hydrogel material in an aqueous medium. This two-component polysaccharide-based hydrogel material has the advantages of being injectable, having low swelling, and having high tissue adhesion. Furthermore, the two-component polysaccharide-based hydrogel material provided by the present invention can be further fabricated into polysaccharide-based biomaterials in the form of membranes, powders, sponges, and other forms, and can be used as tissue adhesives, sealants, hemostats, tissue engineering materials, and other applications, demonstrating its potential for a wide range of biomedical applications.

The invention provides a method for preparing a hydrogel film on a non-conductive surface, and belongs to the technical field of biological material preparation. According to the hydrogel film forming method independent of the electrode surface, a hydrophilic non-conductive substrate is introduced into an electrolytic tank, solutions with different compositions are arranged on the two sides of the non-conductive substrate respectively, an electric field is applied, directional migration of polyelectrolyte molecules is achieved, the polyelectrolyte molecules are deposited on a non-conductive interface, and even if the deposition time is only one minute, the deposition time is not longer than 1 minute. And the generated film still has good uniformity. According to the invention, the dependence limitation of the traditional electrodeposition technology on the conductive substrate is broken through, and a universal platform is provided for constructing functional hydrogel on the surfaces of non-conductive materials such as glass, polymer films and the like. Compared with a traditional method, the method is rapid in preparation, simple in equipment, mild in operation and suitable for various natural and synthetic polyelectrolyte systems, and has huge application potential in the field of biomimetic material preparation.

The application relates to the technical field of biomaterial preparation, and discloses a preparation device and a preparation method of hydrogel microspheres. The preparation device comprises: an aqueous phase preparation unit configured to prepare an aqueous phase solution, the aqueous phase preparation unit being connected with one end of a fluid conveying unit; a reaction emulsification unit configured to be used for oil phase preparation, emulsification and crosslinking reaction, the reaction emulsification unit being connected with the other end of the fluid conveying unit, and the aqueous phase solution being conveyed into the reaction emulsification unit; and a main control system electrically connected with the reaction emulsification unit and the aqueous phase preparation unit. The preparation device can integrate the preparation of the aqueous phase and the oil phase, uniform emulsification, crosslinking solidification and temperature control into one, and can significantly improve the particle size uniformity of the hydrogel microspheres, production efficiency and process stability.

Provided are a TKT gene mutant and an application thereof in the preparation of L-lysine. The TKT gene mutant is a DNA molecule as represented by SEQ ID NO: 3, 5, 7, 9, 11, or 13 in the sequence listing, which encodes a protein as represented by SEQ ID NO: 4, 6, 8, 10, 12, or 14, and a wild type TKT gene is a DNA molecule as represented by SEQ ID NO: 1, which encodes a protein represented by SEQ ID NO: 2. It has been experimentally verified that the TKT gene and the mutant thereof can improve the yield of L-lysine and can be used for the production of L-lysine, and same have good application prospects.

The invention discloses a preparation method and application of injectable photo-crosslinked GelMA / MPDA hydrogel, and belongs to the technical field of medical biological material preparation. The method comprises the following steps: preparing methacrylic anhydride gelatin (GelMA) and polydopamine beads (MPDA) with a mesoporous structure; the preparation method comprises the following steps: mixing a GelMA aqueous solution, MPDA and a photoinitiator, and stirring in a dark place to prepare the injectable photo-crosslinked GelMA / MPDA hydrogel. After being cured, the injectable hydrogel has a uniform honeycomb porous structure and has good injectability and biocompatibility, the photothermal conversion performance of the material is remarkably improved, tumor thermal therapy triggered by near-infrared light is achieved, drug loading and light-controlled release are achieved through mesoporous adsorption and chemical action, and the injectable hydrogel has a good application prospect. Therefore, a novel active controllable drug delivery platform is provided for photothermal-chemical synergistic treatment of tumors.