Preparation method of hyaluronic acid tissue adhesive

A technology of tissue adhesive and hyaluronic acid, which is applied in the preparation of tissue adhesive and hyaluronic acid tissue adhesive, can solve problems such as virus infection, achieve enhanced bonding strength, easy industrial production, The effect of simple preparation method

Active Publication Date: 2017-09-15
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although fibrin-based adhesives have good biocompatibility and degradability, there may be a potential risk of viral infection due to the source of allogeneic human or animal serum, and the safety of its clinical use has attracted widespread attention.

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0026] A preparation method of hyaluronic acid tissue adhesive, said preparation method is carried out according to the following steps:

[0027] a. Preparation of maleylated sodium hyaluronate

[0028] Put sodium hyaluronate and maleic anhydride in dimethylformamide, the mass-volume ratio of sodium hyaluronate to dimethylformamide is 1:4-100, the hydroxyl group on the molecular chain of sodium hyaluronate and maleic anhydride The molar ratio of the acid anhydride group is 1:0.01~0.1, stir evenly at room temperature, react at 25~80°C, and the reaction time is 12~48 hours. After the reaction, sodium hyaluronate, maleic anhydride, dimethyl Add 1mol / L alkali solution to the mixed solution of methyl formamide, adjust the pH value of the mixed solution to 7-8, dialyze the mixed solution after adjusting the pH value, and the dialysis time is 2 days to form maleylated hyaluronic acid sodium hyaluronate solution, freeze-dry the maleylated sodium hyaluronate solution at a temperature ...

Embodiment 1

[0048] Weigh 4g of hyaluronic acid and 0.04g of maleic anhydride, add them into 16mL of dimethylformamide, stir well at room temperature, and react at 25°C for 12 hours. After the reaction, add 1mol / L NaHCO 3 Solution, adjust the pH of the mixed solution to 7-8, dialyze the mixed solution, the dialysis time is 2 days, freeze-dry the dialysate at a temperature of -50°C and a pressure of 1Pa for 24 hours, and obtain the maleoyl mole Sodium maleylated hyaluronate with a degree of substitution of 0.05.

[0049] Weigh 4g of maleylated sodium hyaluronate, add it to 80mL of deionized water, stir at room temperature for 10 hours, add 0.86g of sodium periodate, stir well at room temperature, and react at 25°C for 1 hour , after the reaction, the mixed solution was dialyzed for 2 days, and the dialysate was freeze-dried for 48 hours at a temperature of -50°C and a pressure of 1 Pa to obtain an aldylation with a molar substitution of aldehyde groups of 0.2 Sodium maleylated hyaluronate;...

Embodiment 2

[0053] Weigh 4g of hyaluronic acid and 0.4g of maleic anhydride, add them into 400mL of dimethylformamide, stir well at room temperature, and react at 80°C for 48 hours. After the reaction, add 1mol / L of KHCO 3 solution, adjust the pH of the mixed solution to 7-8, dialyze the mixed solution for 2 days, freeze-dry the dialysate for 72 hours at a temperature of -50°C and a pressure of 20 Pa to obtain the maleoyl mole Sodium maleylated hyaluronate with a degree of substitution of 0.2.

[0054] Weigh 4g of maleylated sodium hyaluronate, add it to 400mL of deionized water, stir at room temperature for 10 hours, add 72.21g of sodium periodate, stir evenly at room temperature, and react at 60°C for 24 hours , after the reaction, the mixed solution was dialyzed for 2 days, and the dialysate was freeze-dried for 48 hours at a temperature of -50°C and a pressure of 20 Pa to obtain a aldehyde group with a molar substitution of aldehyde groups of 0.5. Sodium maleylated hyaluronate;

[0...

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Abstract

The present invention relates to a preparation method of a tissue adhesive, particularly to a preparation method of a hyaluronic acid tissue adhesive, and belongs to the technical field of biological material preparation. According to the preparation method, by modifying the sodium hyaluronate molecule with the maleoyl, the aldehyde group and the dopamine, the grafted dopamine content can be substantially improved, the adhesion effect of the high content dopamine can be completely provided, and the adhesion strength can be synergistically enhanced through the interaction between the residual aldehyde group and the amino in the tissue; with the preparation method, the low-content carbon-carbon double bond substitution can be achieved, and the problems of incomplete adhesion surface sealing and tissue fluid seepage caused by the shrinkage during the subsequent curing of the obtained tissue adhesive can be avoided; and the prepared tissue adhesive has advantages of rapid gelatin under the irradiation of ultraviolet light, safe gelatin, good biocompatibility, high bonding strength, and complete degradation and absorption.

Description

technical field [0001] The invention relates to a preparation method of a tissue adhesive, in particular to a preparation method of a hyaluronic acid tissue adhesive, belonging to the technical field of biomaterial preparation. Background technique [0002] Tissue adhesive is a biomedical material used for sealing and repairing soft tissue injuries such as local tissue adhesion and capillary hemostasis. Its use can replace traditional complicated and time-consuming surgical sutures to quickly seal wounds and avoid wound infection. , Effectively reduce the effect of scar. Ideal tissue adhesives generally have the following conditions: good biocompatibility; fast bonding speed and high bonding strength; low heat during bonding to avoid burning tissues; degradable and absorbable in tissues. [0003] At present, cyanoacrylate adhesives, fibrin adhesives, etc. are widely used clinically. The Chinese patent publication number is CN104958781A, the publication date is October 7, 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/00A61L24/08C08B37/08
CPCA61L24/001A61L24/0042A61L24/08A61L2400/04C08B37/0072C08L5/08
Inventor 周应山李军张灿梁凯利刘欣杨红军柏自奎顾绍金徐卫林
Owner WUHAN TEXTILE UNIV
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