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Method for preparing bacterial cellulose composite fish collagen wound dressing

A technology of fish collagen and bacterial cellulose, applied in peptide preparation methods, chemical instruments and methods, medical science, etc., can solve problems such as restrictions on extracting collagen, doubts about the safety of livestock and poultry collagen products, etc., and achieve a simple preparation process Easy to operate, improve thermal stability and mechanical properties, and promote wound healing

Active Publication Date: 2013-10-23
HAINAN GUANGYU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in recent years, due to the outbreak of mad cow disease, foot-and-mouth disease, bird flu and other diseases, especially the emergence of new subtype influenza viruses such as H5N1 and H7N9, people have serious doubts about the safety of livestock and poultry collagen products
In addition, due to religious and customary reasons, the extraction of collagen from pigskin and cowhide is greatly restricted. Therefore, it is becoming more and more urgent to find new sources of collagen.

Method used

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  • Method for preparing bacterial cellulose composite fish collagen wound dressing
  • Method for preparing bacterial cellulose composite fish collagen wound dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Cut the fish body waste that has been cleaned of surface impurities into small pieces, and cut it into 50-mesh fish meal with a crusher; add distilled water and reaction enzymes that account for 3 times the mass of fish meal in a closed container and stir to make a slurry. The reaction enzyme is composed of neutral lipase and bromelain, the amount of reaction enzyme added accounts for 0.1% of the quality of fish meal, and the mass ratio of neutral lipase and bromelain is 1:4.

[0021] 2. Control the reaction temperature of the prepared slurry to 50° C., pH to 7, and stir for 2 hours to carry out the enzymatic hydrolysis reaction. Add 0.1% sodium hydroxide to the reacted slurry, heat to 90°C, and stir for 20 minutes; filter the slurry, and dialyze the filtrate for 7 days through a dialysis bag with a molecular weight cut-off of 100kDa, and evaporate and concentrate the retained solution to obtain fish collagen protein solution.

[0022] 3. The exudate after dialysis ...

Embodiment 2

[0026] 1. Cut the fish body waste that has been cleaned of surface impurities into small pieces, and cut it into 100-mesh fish meal with a crusher; add distilled water and reaction enzymes that account for 5 times the mass of fish meal in a closed container and stir to make a slurry. The reaction enzyme is composed of neutral lipase and alkaline protease, the amount of reaction enzyme added accounts for 0.3% of the fishmeal mass, and the mass ratio of neutral lipase and alkaline protease is 1:4.

[0027] 2. Control the reaction temperature of the prepared slurry to 55° C., pH to 7.5, and stir for 1.5 hours to carry out the enzymatic hydrolysis reaction. Add 0.2% sodium hydroxide to the reacted slurry, heat to 80°C, and stir for 30 minutes; filter the slurry, and dialyze the filtrate for 7 days through a dialysis bag with a molecular weight cut-off of 150kDa, and evaporate and concentrate the retained solution to obtain fish collagen protein solution.

[0028] 3. The exudate a...

Embodiment 3

[0032] 1. Cut the fish body waste that has been cleaned of surface impurities into small pieces, and cut it into 150-mesh fish meal with a crusher; add distilled water and reaction enzymes that account for 7 times the mass of fish meal in a closed container and stir to make a slurry. The reaction enzyme is composed of neutral lipase and trypsin, the added amount of reaction enzyme accounts for 0.5% of the mass of fish meal, and the mass ratio of neutral lipase and trypsin is 1:3.

[0033] 2. Control the reaction temperature of the prepared slurry to 70° C., pH to 7.5, and stir for 1 hour to carry out the enzymatic hydrolysis reaction. Add 0.3% sodium hydroxide to the reacted slurry, heat to 90°C, and stir for 30 minutes; filter the slurry, and dialyze the filtrate for 7 days through a dialysis bag with a molecular weight cut-off of 200kDa, and evaporate and concentrate the retained solution to obtain fish collagen protein solution.

[0034] 3. The exudate after dialysis is ev...

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Abstract

The present invention discloses a method for preparing bacterial cellulose composite fish collagen wound dressing and relates to a biological material preparation technology. The method comprises: selecting strains capable of secreting bacterial cellulose, activating and preparing seed mash, then inoculating the seed mash with a concentration of 30-90 wt% uniformly into a fermentation medium containing a fish collagen aqueous solution, fermenting for 3-7d and taking out a fermentation product. The fermentation product is purified and cut to obtain the bacterial cellulose composite fish collagen wound dressing which is evenly distributed with fish collagen macromolecules in three-dimensional network. The method is simple in preparation process, easy to operate, low in cost, and the resulting bacterial cellulose composite fish collagen wound dressing has the effects of stopping bleeding, moisturizing, breathability, absorbing wound exudate, and promoting wound healing, and can be used for treating skin wounds such as burn, scald and operative incision.

Description

technical field [0001] The invention relates to a biological material preparation technology, in particular to a preparation method of bacterial cellulose composite fish collagen wound dressing. Background technique [0002] Bacterial cellulose is an excellent biological material with a natural three-dimensional nano-network structure; high tensile strength and elastic modulus; high hydrophilicity, good air permeability, water absorption, water permeability, extraordinary water holding capacity and high wet strength . In addition, a large number of studies have shown that bacterial cellulose has good biocompatibility and biodegradability in vivo and in vitro, which makes bacterial cellulose itself suitable for biomedical applications. Bacterial cellulose is used as the base material of the dressing. Using its own water absorption performance, it can continuously and effectively absorb wound exudate and metabolites on the basis of ensuring biological safety, and can provide ...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/28C07K1/36C07K1/12C07K1/34
Inventor 钟春燕钟宇光
Owner HAINAN GUANGYU BIOTECH
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