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Soft and hard tissue integrated regeneration-repair gradient three-dimensional fibrous scaffold and preparation method thereof

A fiber scaffold and hard tissue technology, applied in tissue regeneration, fiber treatment, prosthesis, etc., can solve the problems of brittleness, poor plasticity, difficult to meet the requirements of tissue regeneration and repair, and affect the prospects The effect of maintaining appearance and improving mechanical properties

Active Publication Date: 2020-02-11
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inherent brittleness and poor plasticity of ceramic materials affect the prospect of their application as bone tissue engineering scaffolds alone.
[0004] It can be seen that it is difficult for a single material to meet the requirements of tissue regeneration and repair, especially when repairing soft and hard tissue defects with gradient changes in composition and function. Tissue engineering scaffolds with gradient structure put forward higher requirements for the selection of materials and scaffold preparation methods

Method used

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  • Soft and hard tissue integrated regeneration-repair gradient three-dimensional fibrous scaffold and preparation method thereof
  • Soft and hard tissue integrated regeneration-repair gradient three-dimensional fibrous scaffold and preparation method thereof
  • Soft and hard tissue integrated regeneration-repair gradient three-dimensional fibrous scaffold and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Weigh 0.5g of L-polylactic acid and gelatin respectively, add them together to 10ml of trifluoroethanol, stir in a water bath at 37°C for 24h, and rotate at 400rpm to obtain a stable polymer blend solution without obvious phase separation ;

[0039] (2) Spin the blended solution in step (1) by electrospinning process, and receive it on a metal plate to obtain a non-woven fiber membrane. The spinning parameters are: voltage 20kV, receiving distance 15cm, and flow rate 0.8ml / h;

[0040] (3) 0.4g of the fibrous film obtained in step (2) is cut into fragments of 0.5cm×0.5cm, suspended in 20ml of tert-butanol, and sheared at 14000rpm for 20min with a high-speed dispersing homogenizer to obtain uniformly dispersed Fiber suspension, add 8g sodium chloride as porogen and disperse evenly;

[0041] (4) Transfer the suspension obtained in step (3) to a mold (such as a 48-well plate), then freeze at -20°C for 24 hours, and then freeze-dry for 48 hours to obtain a disc-shaped t...

Embodiment 2

[0047] (1) With embodiment 1 step (1)-(5).

[0048] (2) Soak the obtained polylactic acid / gelatin three-dimensional fiber scaffold in the prepared 5-fold simulated body fluid, soak at 37°C for 24 hours, take out the scaffold and wash it with deionized water, then freeze at -20°C for 24 hours, and then freeze-dry After 48 hours, a three-dimensional fiber scaffold in the middle layer with a mineral deposition amount of 5-20 wt.% was obtained.

Embodiment 3

[0050] (1) With embodiment 1 step (1)-(5).

[0051] (2) Soak the obtained polylactic acid / gelatin three-dimensional fiber scaffold in the prepared 5-fold simulated body fluid, soak at 37°C for 48 hours, take out the scaffold and wash it with deionized water, then freeze at -20°C for 24 hours, and then freeze-dry After 48 hours, a three-dimensional fibrous scaffold with a mineral deposition amount of 20-40wt.% of the hard tissue layer was obtained.

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Abstract

The invention relates to a soft and hard tissue integrated regeneration-repair gradient three-dimensional fibrous scaffold and a preparation method thereof, and belongs to the technical field of preparation of medical biomaterials. According to the gradient three-dimensional fibrous scaffold, fiber lap joint is achieved, the porosity is greater than 90%, a perforated macroporous structure with thepore diameter being 30-150 [mu]m is included, the compressive strength reaches the MPa level, and the composition of the gradient three-dimensional fibrous scaffold from the top layer of the scaffoldto the bottom layer of the scaffold is of a bionic natural soft and hard tissue transition structure; and fibers are prepared from natural polymers with biocompatibility and synthetic polymers with synthetic polymer in a composited mode, wherein glycosaminoglycan components are added in composite fibers of the soft tissue layer scaffold, and hydroxylapatite components are deposited on the surfaces of composite fibers of a middle transition layer and a hard tissue layer scaffold. The gradient scaffold provided with different functional layers and meeting soft and hard tissue regeneration-repair demands is prepared, and cells and tissue in the scaffold can be induced in a layered mode to be simultaneously subjected to soft tissue differentiation and osteoblastic differentiation.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterial preparation, and relates to a three-dimensional scaffold for regeneration and repair of soft and hard tissues and a preparation method thereof, in particular to a bionic gradient three-dimensional fiber scaffold for integrated regeneration and repair of soft and hard tissues and a preparation method thereof. Background technique [0002] Due to sports trauma or disease, soft tissue and hard tissue damage may be caused at the same time in certain parts, such as osteochondral, tendon bone and other parts with complex gradient structures. The natural joint osteochondral structure is mainly divided into hyaline cartilage layer, calcified cartilage layer, and subchondral bone; the normal tendon-bone junction is composed of tendon tissue, uncalcified fibrocartilage, calcified fibrocartilage, and bone tissue. Different parts have obvious differences in composition, structure and cell componen...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/20A61L27/18A61L27/22A61L27/32D01D5/00D04H1/728
CPCA61L27/18A61L27/20A61L27/222A61L27/32A61L27/56A61L2430/02A61L2430/06A61L2430/10D01D5/003D04H1/728C08L5/00C08L67/04
Inventor 蔡晴方嘉锦赵文文杨小平
Owner BEIJING UNIV OF CHEM TECH
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