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Porous scaffold for bone repair and its rapid prototyping method

A porous scaffold and molding method technology, applied in the fields of biomedical materials technology and biomedical engineering, can solve problems such as single production components, complex and variable application requirements, and difficulty in meeting clinical treatment, so as to promote bone healing and good biocompatibility sexual effect

Active Publication Date: 2017-03-22
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing rapid prototyping technology is also limited to the production of bone scaffold materials with a single component, which is difficult to meet the complex and changeable application requirements of clinical treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Preparation of PLGA microspheres loaded with rhBMP-2

[0042] Take one volume part of 50mg / mL rhBMP-2 ultrapure aqueous solution to ten volume parts of PLGA (molecular weight 50kDa, lactic acid and glycolic acid polymerization molar ratio is 75 / 25) dichloromethane (DCM) solution, and use high-speed dispersion Emulsify at a speed of 3000rpm for 60 seconds to obtain an inner emulsion (w / o); add 0.3mL Tween 80 to 30mL deionized water, stir until uniformly dispersed, and obtain an outer water phase; add the inner emulsion to the outer water phase , use a high-speed disperser to emulsify at a speed of 5000rpm for 60 seconds to obtain a double emulsion (w / o / w); stir the liquid at a medium speed for 3-5 hours until the DCM is volatilized, and let it stand overnight. After being centrifuged, washed with water three times to obtain a suspension of PLGA microspheres loaded with rhBMP-2, and freeze-dried to obtain dry microspheres for future use.

[0043] 2. Preparation of PLA...

Embodiment 2

[0048] 1. Preparation of chitosan-loaded BMP-14 microspheres

[0049] Take 1500 parts by mass of chitosan (molecular weight 250kDa, degree of deacetylation 90%) dissolved in an appropriate amount of 20ml / L acetic acid solution, take 1 mass part of BMP-14 dissolved in an appropriate amount of 4mol / L HCl solution, mix Then slowly add 90~200ml span-80 (5ml) octanol solution, add 1~3ml genipin dropwise for cross-linking during magnetic stirring, continue stirring for 40~60min to stop the reaction, and use isopropanol, petroleum ether Wash with water repeatedly to obtain chitosan-loaded BMP-14 microsphere suspension. Dry microspheres were obtained after freeze-drying.

[0050] 2. Preparation of BMP-14-loaded microsphere composites

[0051] Dissolve three parts by mass of PLA (molecular weight 100kDa) in an appropriate amount of DCM solution, add one part by mass of β-tricalcium phosphate (TCP) powder and 50-100mg BMP-14 chitosan microspheres, stir and disperse to obtain a composi...

Embodiment 3

[0055] 1. Preparation of PLA microspheres loaded with TGF-α

[0056] Take the ultrapure aqueous solution of the TGF-α of one volume part of 50mg / mL to the DCM solution of the PLA of ten volume parts, emulsify 60 seconds with the rotating speed of 3000rpm with a high-speed disperser, make inner emulsion (w / o); In 30mL Add 0.3mL Tween 80 to deionized water, stir until uniformly dispersed to obtain the outer water phase; add the inner emulsion to the outer water phase, and emulsify with a high-speed disperser at a speed of 5000rpm for 60 seconds to obtain a double emulsion (w / o / w); the solution was stirred at a medium speed for 3-5 hours until the DCM was volatilized, and left to stand overnight. After centrifugation, wash with water three times to obtain PLA microsphere suspension loaded with TGF-α. Dry microspheres were obtained after freeze-drying.

[0057] 2. Preparation of PLG / HA three-dimensional scaffold matrix

[0058] Take three parts by mass of PLG (molecular weight...

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Abstract

The invention relates to a bone repair porous bracket, which comprises a substrate with a bionic porous structure and growth factor controlled-release microspheres, wherein the growth factor controlled-release microspheres are adsorbed into gaps of the bionic porous structure on the substrate or are dispersed into the substrate by being uniformly mixed with substrate raw material in a substrate forming process. The invention further provides a rapid forming method for the bone repair porous bracket. The method comprises the following steps of: preparing growth factor controlled-release microspheres; forming a substrate; and adsorbing. The invention further provides another rapid forming method for the bone repairing porous bracket. The method comprises a step for preparing growth factor controlled-release microspheres and a step for forming a substrate. The growth factor controlled-release microspheres are introduced into the bracket, bone growth can be induced under the action of the controlled release of growth factors, and inward bone tissue growth is facilitated through a porous structure formed by degradation of the controlled-release microspheres, so that bone tissue regeneration repair is achieved, and bone healing is facilitated effectively. Three-dimensional printing is performed by adding controlled-release microspheres and adopting a rapid forming technology, and a forming process is simple and rapid.

Description

technical field [0001] The invention relates to the fields of biomedical material technology and biomedical engineering, more precisely, the invention relates to a porous scaffold for bone repair and a rapid prototyping method thereof. Background technique [0002] At present, clinically, the incidence of bone defects due to trauma, tumor resection, infection and abnormal development is relatively high. In the existing methods, there are few sources of autologous bone transplantation, and it will bring certain pain to patients; allogeneic bone transplantation There are rejection problems and ethical constraints limit its application. Bone tissue engineering based on biomaterials has brought new ideas to the generation of new bone scaffold materials, and brought hope for orthopedic and repair of bone defects. However, the existing porous scaffolds for artificial bone repair have a single component, which is difficult to meet the requirements of bone repair. [0003] In addi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/50A61L27/54
Inventor 王明波储彬佘振定
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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