Preparation method of alginic acid hydrogel matrix for UV 3D printing

A 3D printing and alginic acid technology, applied in medical science, prosthesis, etc., can solve the problems of reduced 3D printing efficiency, difficulty in sol shaping, difficulty in maintaining size, etc., to achieve good adhesion, low cost, and increased grafting rate Effect

Inactive Publication Date: 2016-09-14
济南科乐医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method improves the strength of 3D printed alginic acid hydrogel, due to the long time of the first chemical crosslinking, the printed sol is difficult to shape, difficult to maintain size, and prone to collapse
In addition, the operation is cumbersome, which greatly reduces the efficiency of 3D printing

Method used

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  • Preparation method of alginic acid hydrogel matrix for UV 3D printing
  • Preparation method of alginic acid hydrogel matrix for UV 3D printing
  • Preparation method of alginic acid hydrogel matrix for UV 3D printing

Examples

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preparation example Construction

[0025] a. Preparation of gelatinized sodium alginate

[0026] Put sodium alginate and aprotic solvent in deionized water, the mass volume ratio of sodium alginate to deionized water is 1:10-200, the volume ratio of aprotic solvent to deionized water is 1:10-50, stir at room temperature For 1 to 24 hours, dry the mixed solution of sodium alginate, aprotic solvent, and deionized water at a temperature of -10 to -40°C and a pressure of 10 to 100 Pa for 18 to 72 hours to obtain gelatinized alginic acid sodium. The aprotic solvent is one of dimethylformamide, dimethylacetamide or dimethylsulfoxide.

[0027] Sodium alginate is also known as sodium alginate, kelp gum, and alginate. It is a natural polysaccharide isolated from brown seaweed such as kelp and sargassum. It is composed of different proportions of β-L-mannuronic acid (M unit) and α-D-guluronic acid (G unit) connected by 1,4-glycosidic linkages, composed of different GG, MM, GM segments. Due to its superior biocompatibi...

Embodiment 1

[0041] Weigh 5 g of sodium alginate, add it to 50 mL of deionized water, add 50 mL of dimethylformamide, stir at room temperature for 1 hour, place the solution at -10°C and a pressure of 10 Pa, and dry it for 18 hours to obtain a gelatinized sodium alginate.

[0042] Weigh 5g of gelatinized sodium alginate and 0.5g of maleic anhydride, add them to 50mL of dimethylformamide, stir well at room temperature, and react at 25°C for 12 hours. After the reaction, add anhydrous acetone until no precipitation Until the precipitation, the precipitate was collected and dried in vacuum at room temperature for 2 days to obtain maleic anhydride-grafted sodium alginate with a molar substitution of 0.05.

[0043] Weigh 1g of maleic anhydride grafted sodium alginate, 0.01g of calcium chloride, 0.05g of 2-hydroxy-2-methyl-1-p-hydroxyethyl ether phenylacetone, and add to 98.94g of Na with a pH of 7.0 2 HPO 4 -NaH 2 PO 4 In the buffer solution, mix uniformly at room temperature to obtain an a...

Embodiment 2

[0045] Weigh 5 g of sodium alginate, add it to 1000 mL of deionized water, add 250 mL of dimethylacetamide, stir at room temperature for 24 hours, place the solution at -40°C and a pressure of 100 Pa, and dry it for 72 hours to obtain gelatinization sodium alginate.

[0046] Weigh 5g of gelatinized sodium alginate and 50g of maleic anhydride, add them to 1000mL of dimethylacetamide, stir well at room temperature, and react at 90°C for 48 hours. After the reaction, add anhydrous acetone until no precipitation occurs The precipitate was collected and dried in vacuum at room temperature for 2 days to obtain maleic anhydride-grafted sodium alginate with a molar substitution of 0.8.

[0047] Weigh 10 g of maleic anhydride grafted sodium alginate, 0.05 g of zinc chloride, and 0.1 g of 1-hydroxycyclohexyl phenyl ketone, and add it to 89.85 g of K with a pH of 7.4. 2 HPO 4 -KH 2 PO 4 In the buffer solution, mix uniformly at room temperature to obtain an alginic acid hydrogel matri...

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Abstract

The invention relates to a preparation method of a hydrogel matrix, especially a preparation method of an alginic acid hydrogel matrix for UV 3D printing, and belongs to the technical field of biological material preparation. According to the preparation method, alkenyl groups are grafted on the molecular chains of sodium alginate prepared by a freeze-dry method, and after dissolving and mixing, the alginic acid hydrogel matrix for UV 3D printing is obtained. The preparation method adopts a freeze-drying technology under mixed solvent environment, the grafting rate of sodium alginate is largely improved, and the problem that the UV curing speed is slow due to low grafting rate is completely solved. The prepared alginic acid hydrogel matrix can rapidly solidify under the irradiation of UV light, the solid state can be maintained, moreover, in the presence of low concentration metal halides, the system can be further solidified, the hydrogel strength is improved, and the problems of conventional 3D printing hydrogel such as difficult moulding, collapse, and the like are overcome. The preparation method is simple, the cost is low, and the preparation method can be applied to industry easily.

Description

technical field [0001] The invention relates to a method for preparing a hydrogel matrix, in particular to a method for preparing an alginic acid hydrogel matrix for ultraviolet 3D printing, and belongs to the technical field of biomaterial preparation. Background technique [0002] Hydrogel is a kind of hydrophilic network polymer swelling body that can swell in water, absorb and retain a large amount of water without dissolving in water. Because of its high water content and structure similar to extracellular matrix, it is suitable for cell adhesion, growth and proliferation. It has become one of the preferred substrates for 3D tissue printing and artificial organ preparation, and has therefore become a major player in chemistry, materials and life medicine. research hotspots. [0003] 3D printing alginate hydrogel, generally using sodium alginate sol mixed with CaCl 2 The solution is sprayed and printed by layer-by-layer method. However, this method only prepares hydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08F251/00C08F222/06C08L51/02A61L27/20A61L27/50A61L27/52
CPCA61L27/20A61L27/50A61L27/52C08F251/00C08J3/075C08J3/24C08J2351/02C08L51/02C08F222/06C08L5/04
Inventor 周应山董齐张灿殷先泽杨红军柏自奎顾绍金陶咏真徐卫林
Owner 济南科乐医药有限公司
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