A kind of preparation method of xylo-oligosaccharide

A technology of xylooligosaccharides and xylans, which is applied in the direction of fermentation, can solve the problems of resource waste, environmental pollution, and low overall use value, and achieve the effect of reducing pretreatment costs, reducing environmental pollution, and improving comprehensive economic benefits

Active Publication Date: 2021-10-15
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the bagasse is directly burned as boiler fuel, and a small part is used as raw materials for wood-based panels and pulp and paper making. The overall use value is low, which not only causes environmental pollution, but also leads to waste of resources.

Method used

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  • A kind of preparation method of xylo-oligosaccharide
  • A kind of preparation method of xylo-oligosaccharide
  • A kind of preparation method of xylo-oligosaccharide

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Embodiment 1

[0046] The present embodiment relates to a kind of preparation method of xylo-oligosaccharide, comprises the following steps:

[0047] 1) Wash and air-dry the bagasse, pulverize it with a pulverizer, and sieve it to 20 meshes to control its water content to ≤5%. On a dry basis, put 10kg of bagasse in a pressure-resistant reactor, and then add 100L of 50% to the reactor (v / v) ethanol aqueous solution, add 1.0kg sodium hydroxide at the same time, heat to 100 ℃ after sealing, process 2h, cool down, obtain filtrate and filter residue after filtering;

[0048] 2) After the filtrate is concentrated by rotary evaporation, the filtrate is subjected to alcohol precipitation with 3 times the volume of 95% (v / v) ethanol solution to obtain a xylan precipitation; the xylan is filtered to obtain a secondary filtrate, which is subjected to rotary concentration , recovery of ethanol; then the lignin is separated by acid precipitation, and the residue after the separation of lignin is burned t...

Embodiment 2

[0052] The present embodiment relates to a kind of preparation method of xylo-oligosaccharide, comprises the following steps:

[0053] 1) Wash and air-dry the bagasse, pulverize it with a pulverizer, and sieve it into 20 meshes to control its water content to ≤5%. On a dry basis, put 10kg of the bagasse in a pressure-resistant reaction kettle, and then add 100L of 50% (v / v) ethanol aqueous solution, add 1.5kg sodium hydroxide at the same time, heat to 110 ℃ after sealing, treat for 1.5h, cool down, obtain filtrate and filter residue after filtering;

[0054] 2) After the filtrate is concentrated by rotary evaporation, it is extracted with 3 times the volume of 95% (v / v) ethanol precipitation to obtain a xylan precipitate; the secondary filtrate obtained after filtering the xylan is subjected to rotary concentration and recovery Ethanol; then the lignin in it is separated by acid precipitation, and the residue after the separation of lignin is burned to recover alkali and heat ...

Embodiment 3

[0058] The present embodiment relates to a kind of preparation method of xylo-oligosaccharide, comprises the following steps:

[0059] 1) Wash and air-dry the bagasse, pulverize it with a pulverizer, and sieve it into 20 meshes to control its water content to be ≤5%. On a dry basis, put 10 kg of the bagasse in a pressure-resistant reaction kettle, add 100L of 50% (v / v) ethanol aqueous solution, add 0.5kg sodium hydroxide at the same time, heat to 100 ℃ after sealing, process 1h, cool down, obtain filtrate and filter residue after filtering;

[0060] 2) After the filtrate is concentrated by rotary evaporation, it is extracted with 3 times the volume of 95% (v / v) ethanol precipitation to obtain a xylan precipitate; the secondary filtrate obtained after filtering the xylan is subjected to rotary concentration and recovery Ethanol; then the lignin in it is separated by acid precipitation, and the residue after the separation of lignin is burned to recover alkali and heat energy. ...

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Abstract

The invention relates to a preparation method of xylo-oligosaccharides, the main innovations of which are the pretreatment of lignin fiber materials with an alkali-ethanol solution, and the extraction of xylans with a subcritical water extraction method. The pretreatment method of the present invention can remove a large amount of lignin, reduce the bondage of lignin to carbohydrates, destroy the internal compact structure that hinders the enzymatic hydrolysis and saccharification of endoxylanase; it can also destroy the crystal structure of carbohydrates, and make biomass The contact between the raw material and the biocatalyst is more sufficient, and the enzymatic hydrolysis efficiency of the endoxylanase is improved. The xylan prepared by the method of the present invention has high purity and high yield, and the amount of endoxylanase used in the preparation of xylo-oligosaccharides in the subsequent process is small; and the cellulose content of the residue after subcritical water extraction of xylan is high , is a high-quality raw material for the preparation of fiber materials.

Description

technical field [0001] The invention belongs to the field of biomass chemistry and chemical industry, and in particular relates to a preparation method of xylooligosaccharides. Background technique [0002] Raw materials such as poplar, eucalyptus and bagasse are rich in xylan, and the conversion of xylan into xylooligosaccharides can effectively increase the utilization value of biomass. Poplar and eucalyptus are widely planted plantation resources in my country. The leaves, branches and sawdust are rich in hemicellulose. Bagasse is the main by-product of sugarcane sugar industry, accounting for 24-27%. The fibrous residue after extracting cane juice from sugarcane through crushing and pressing process is a renewable agricultural solid waste. The main components of bagasse are cellulose (30-45%), hemicellulose (25-45%) and lignin (15-30%). As the third largest sugarcane planting country in the world after Brazil and India, my country has an annual output of 13.5 million ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
CPCC12P19/14
Inventor 蒋建新游艳芝张威伟杨淑娟朱莉伟
Owner BEIJING FORESTRY UNIVERSITY
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