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Fibroin medical biological adhesive and preparation method thereof

A bio-adhesive, silk fibroin technology, applied in pharmaceutical formulations, surgical adhesives, applications, etc., can solve the problems of low adhesive strength, long coagulation time, troublesome storage, etc., to achieve super adhesion performance, good Biocompatible, easy-to-use effect

Active Publication Date: 2018-11-06
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention overcomes the problems of low bonding strength, long setting time and troublesome storage of existing bioadhesives, and the prepared silk fibroin medical bioadhesive can be used for almost all trauma wounds, and has the advantages of bonding tissues, covering Wounds, filling defects and gaps, hemostasis, antibacterial, etc.

Method used

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  • Fibroin medical biological adhesive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of silk fibroin solution

[0019] After heating 2L of deionized water to boiling, add 6 g of sodium carbonate and wait until it is fully dissolved. Put 5 g silkworm raw silk into it, and degummed it at 100 °C. Wash thoroughly with deionized water and dry in an oven at 60 °C. The dried degummed silk fibroin fibers were dissolved in LiBr solution at 60 °C for 6 h. The dissolved solution was put into a dialysis bag and dialyzed against deionized water for 72 h. The dialyzed solution was filtered and centrifuged to obtain a silk fibroin solution with a mass fraction of about 20% after repeated twice. 10 g of catechol was dissolved in slightly alkaline Tris-HCl buffer and stirred for 0.5 h to obtain a 10% catechol solution. At room temperature, 10% catechol solution and 20% silk fibroin solution were uniformly mixed for 0.5 h at a mass ratio of 800:1 to obtain a silk fibroin adhesive with adhesive properties. The adhesive can be used for hemostatic adhesion ...

Embodiment 2

[0021] After heating 10L of deionized water to boiling, add 50g of sodium carbonate and wait until it is fully dissolved. Put 25 g silkworm raw silk into it, and degumming process at 90 °C. Wash thoroughly with deionized water and dry in an oven at 50 °C. The dried degummed silk fibroin fibers were dissolved in LiBr solution at 80 °C for 3 h. The dissolved solution was put into a dialysis bag and dialyzed against deionized water for 60 h. The dialyzed solution was filtered and centrifuged to obtain a silk fibroin solution with a mass fraction of about 40% after repeated twice. 500 g of tannic acid was dissolved in slightly alkaline Tris-HCl buffer and stirred for 50 h to obtain a 500% tannic acid solution. At room temperature, a 500% tannic acid solution and a 40% silk fibroin solution were uniformly mixed for 30 h at a mass ratio of 1:1 to obtain a silk fibroin adhesive with adhesive properties. The adhesive can be used for hemostatic adhesion of organs, bones, joints and...

Embodiment 3

[0023] After heating 15L of deionized water to boiling, add 100 g of sodium carbonate and wait for it to fully dissolve. Put 15 g silkworm raw silk into it, and degummed it at 80 °C. Wash thoroughly with deionized water and dry in an oven at 70 °C. The dried degummed silk fibroin fibers were dissolved in a calcium chloride / absolute ethanol / water ternary solution at 60°C for 6 h. The dissolved solution was put into a dialysis bag and dialyzed against deionized water for 40 h. The dialyzed solution was filtered and centrifuged to obtain a silk fibroin solution with a mass fraction of about 5% after repeated twice. Dissolve 1 g of epicatechin in water and stir for 30 h to obtain a 1% epicatechin solution. At room temperature, a 1% epicatechin solution and a 5% silk fibroin solution were uniformly mixed for 50 h at a mass ratio of 1:600 ​​to obtain a silk fibroin adhesive with adhesive properties. The adhesive can be used for hemostatic adhesion of tissues such as nerves, muco...

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Abstract

The invention discloses a fibroin medical biological adhesive and a preparation method thereof, and belongs to the field of biomaterial preparation and biomedical application. The adhesive is preparedby uniformly mixing a fibroin solution and a polyphenolic polymer solution at room temperature. The fibroin biological adhesive has the advantages of high adhesion strength, high adhesion speed, safety, no toxicity, good biocompatibility and low price in a humid environment. The adhesive is simple to operate and easy to grasp, and suture removal and other secondary operations are not required after an operation to relieve the pain of a patient. The small or even scar-free wound surfaces have a cosmetic effect. The adhesive can be used on almost all wound surfaces, has the effects of bonding tissues, covering the wound surfaces, filling gaps and voids, stopping bleeding, inhibiting bacteria and the like, can be used as a hemostatic adhesive material for tissues such as skins, organs, bloodvessels, nerves, muscles, mucous membranes, bones, joints and eyes, and has extensive clinical application prospects.

Description

technical field [0001] The invention belongs to the field of biomaterial preparation and biomedical application, and in particular relates to a silk fibroin medical bioadhesive and a preparation method thereof. Background technique [0002] According to statistics, millions of people need surgical treatment every year due to trauma, which mainly includes skin, muscle, connective tissue, internal organs, bone and so on. The most commonly used method clinically is to suture the wound with sutures, rivets and other materials. This method ensures that the suture is firm and the wound is not easy to crack. Patients have thread-knot reactions to varying degrees, leaving "centipede"-like scars after surgery. With the rapid development of modern medicine, the clinical requirements for surgical methods and auxiliary materials are getting higher and higher, not only to reduce the pain of patients to the greatest extent, but also to restore the appearance perfectly while restoring the...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/00A61L24/04
CPCA61L24/001A61L24/046A61L24/108A61L2400/04C08L89/00
Inventor 白树猛张雪亮杨黄浩黄晓卫
Owner FUZHOU UNIV
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