Preparation method of drug-carrying hydroxylapatite microspheres and bone cement composite porous microspheres

A technology of hydroxyapatite and nano-hydroxyapatite, which is applied in the fields of phosphorus compounds, chemical instruments and methods, and medical science, and can solve problems such as the influence of in-situ drug loading, difficult drug loading, and unstable drug release. , to achieve the effects of easy regulation of microsphere size, control of drug release rate, and adjustable drug loading

Inactive Publication Date: 2009-02-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the dissolution process, it is easy to cause the collapse of the microspheres, which has a certain impact on the drug loading in situ
Another type of drug carrier is sintered microspheres. By heat-treating microspheres without in-situ drug wrapping, sintered microspheres with high strength, high porosity, and through holes can be obtained. This type of calcium phosphate ceramic microspheres mainly passes Adsorption for drug loading, the drug loading dose is small, and it is difficult to precisely control the drug loading amount, and the drug release is unstable

Method used

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  • Preparation method of drug-carrying hydroxylapatite microspheres and bone cement composite porous microspheres
  • Preparation method of drug-carrying hydroxylapatite microspheres and bone cement composite porous microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1: Synthesis of nano-hydroxyapatite

[0019] Accurately weigh 23.66gCa(NO 3 ) 2 ·4H 2 O and 4.26g P 2 O 5 , The corresponding Ca(NO 3 ) 2 ·4H 2 O and P 2 O 5 Dissolved into 300ml and 125ml absolute ethanol, respectively, prepared into the molar concentration of 0.3mol / L and 0.24mol / L Ca(NO 3 ) 2 And P 2 O 5 Solution. Using 100ml absolute ethanol as the base liquid, put P 2 O 5 And Ca(NO 3 ) 2 The solution was dripped into the base liquid at the same time, stirred continuously to obtain a transparent solution, and stood for 24 hours to obtain a milky white gel-like system. It was then dried in an oven at 70°C to obtain a white powder. Then put the white powder obtained in a calcination furnace and heat to 300°C to obtain hydroxyapatite with a low degree of crystallinity. Calcined at 600°C, hydroxyapatite with complete crystals can be obtained, and the calcined powder has good dispersibility. .

[0020] Step two:

[0021] Accurately weigh 171.1 grams of calcium hydrogen p...

Embodiment 2

[0027] Step one and step two are the same as in Example 1.

[0028] Step 3: Preparation of hydroxyapatite porous microspheres

[0029] Dissolve 4.5g of gelatin in 30ml of deionized water at a temperature of 40°C and stir for 1 hour to prepare a gelatin solution with a concentration of 0.15g / ml; accurately weigh out 9g of nano-hydroxyapatite prepared in step 1 of Example 1. Stone (HAp) powder was added to the above gelatin solution to prepare a gelatin / HAp mixed suspension with a concentration of 0.3 g / ml. The gelatin / HAp mixed suspension was added to soybean oil at a stirring speed of 700 rpm, and the oil temperature was kept at 15°C. After stirring for 1 hour, let the microspheres settle on the bottom of the oil layer by standing for 24 hours; pour the upper layer of vegetable oil, wash the prepared microspheres with acetone and ethanol respectively, and dry them naturally in the air to obtain gelatin / HA composite microspheres; place the composite microspheres on the bottom of th...

Embodiment 3

[0033] Step one and step two are the same as in Example 1.

[0034] Step 3: Preparation of hydroxyapatite porous microspheres

[0035] Dissolve 8g of gelatin in 40ml of deionized water at a temperature of 40°C and stir for 1 hour to prepare a gelatin solution with a concentration of 0.2g / ml; accurately weigh 16g of the nano-hydroxyapatite prepared in step 1 of Example 1 ( HAp) powder is added to the above gelatin solution to prepare a gelatin / HAp mixed suspension with a concentration of 0.4 g / ml. The gelatin / HAp mixed suspension was added to soybean oil at a stirring speed of 700 rpm, and the oil temperature was kept at 15°C. After stirring for 1 hour, let the microspheres settle for 36 hours at the bottom of the oil layer; pour the upper layer of soybean oil, wash the prepared microspheres with acetone and ethanol, and dry them naturally in the air to obtain gelatin / HA composite microspheres; place the composite microspheres on the bottom of the oil layer. Sintering is carried ou...

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Abstract

The invention discloses a preparation method of porous microspheres composite with hydroxyapatite microspheres being capable of carrying drugs and bone cement, which belongs to the technical field of biological material preparation. The method comprises the following steps: the preparation of nano hydroxyapatite powders; the preparation of Alpha- tricalcium phosphate powders; the preparation of the hydroxyapatite microspheres; and the preparation of the porous microspheres composite with the hydroxyapatite microspheres and the bone cement. The method has the advantages that: the porosity of the porous microspheres composite with the hydroxyapatite microspheres and the bone cement, and the size of the microspheres can be easily controlled; the composite of the drugs can be carried out by the preparation process of the bone cement stuff, the drug-carrying rate is large and controllable; and the composite porous microspheres can be served as drug carriers or stents with integration of bone restoration and cure.

Description

Technical field [0001] The invention relates to a method for preparing drug-carrying hydroxyapatite microspheres and bone cement composite porous microspheres, and belongs to the technical field of biological material preparation. Background technique [0002] As a drug carrier material for orthopedics, inorganic biomaterials based on calcium phosphate have similar chemical composition to bone tissue and have osteoconducting properties. Compared with other orthopedic drug carrier materials, they have irreplaceable advantages. Calcium phosphate materials as drug carriers mainly include stents and powders. Scaffold materials are difficult to form according to the shape of the lesion, which limits their application, and powder materials also have obvious disadvantages due to their drug-carrying capacity and weak erosion resistance. The granular drug carrier has higher drug activity, such as the expected therapeutic effect, high drug utilization, long drug release cycle and controlla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/02C01B25/32A61L27/12A61L27/02A61L27/56A61L27/54
Inventor 蔡舒李树利许国华周维
Owner TIANJIN UNIV
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