Fibroin bone cement biological adhesive and preparation method thereof

A bioadhesive, silk fibroin technology, used in surgical adhesives, tissue regeneration, drug delivery, etc., can solve the problem of poor biocompatibility, non-biodegradable calcium phosphate bone cement, poor water solubility, and early bonding strength. Insufficient and other problems, to achieve the effect of promoting tissue repair, excellent wet adhesion, and low price

Active Publication Date: 2018-11-06
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of poor biocompatibility, non-biodegradability, poor water solubility of calcium phosphate bone cement, and insufficient early bonding strength of existing polymethyl methacrylate bone cement, and to provide a method that can be used in a wet physiological environment. Bone cement bioadhesive capable of producing immediate effective bonding and stable mechanical support performance at the injury site and preparation method thereof

Method used

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  • Fibroin bone cement biological adhesive and preparation method thereof

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Embodiment 1

[0019] After heating 2L of deionized water to boiling, add 6 g of sodium carbonate and wait until it is fully dissolved. Put 5 g silkworm raw silk into it, and degummed it at 100 °C. Wash thoroughly with deionized water and dry in an oven at 60°C. The dried degummed silk fibroin fibers were dissolved in LiBr solution at 60 °C for 6 h. The dissolved solution was put into a dialysis bag and dialyzed against deionized water for 72 h. The dialyzed solution was filtered and centrifuged to obtain a silk fibroin solution with a mass fraction of 20% after repeated twice. 10 g of catechol was dissolved in Tris-HCl buffer and stirred at room temperature for 1 h to prepolymerize catechol in air to obtain a catechol solution with a mass fraction of 10%. Add 10 g of hydroxyapatite nanoparticles into the catechol solution with a mass fraction of 10%, mix evenly according to the mass ratio of 600:1, and stir for 3 h to make the catechol adsorb on the surface of the nanoparticles to obtain...

Embodiment 2

[0021] After heating 10L of deionized water to boiling, add 50g of sodium carbonate and wait until it is fully dissolved. Put 25 g silkworm raw silk into it, and degumming process at 90 °C. Wash thoroughly with deionized water and dry in an oven at 50 °C. The dried degummed silk fibroin fibers were dissolved in LiBr solution at 80°C for 3 h. The dissolved solution was put into a dialysis bag and dialyzed against deionized water for 60 h. The dialyzed solution was filtered and centrifuged, and repeated twice to obtain a silk fibroin solution with a mass fraction of 40%. 500 g of tannic acid was dissolved in water and stirred at room temperature for 50 h to prepolymerize tannic acid in air to obtain a tannic acid solution with a mass fraction of 500%. Add 800g of calcium phosphate nanoparticles into the tannic acid solution with a mass fraction of 500%, mix evenly according to the mass ratio of 1:1, and stir for 60 h to make the tannic acid adsorb on the surface of the nanopa...

Embodiment 3

[0023] After heating 15L of deionized water to boiling, add 100 g of sodium carbonate and wait for it to fully dissolve. Put 15 g silkworm raw silk into it, and degummed it at 80 °C. Wash thoroughly with deionized water and dry in an oven at 70°C. The dried degummed silk fibroin fibers were dissolved in a calcium chloride / absolute ethanol / water ternary solution at 60°C for 6 h. The dissolved solution was put into a dialysis bag and dialyzed against deionized water for 40 h. The dialyzed solution was filtered and centrifuged, and repeated twice to obtain a silk fibroin solution with a mass fraction of 5%. 1 g of epicatechin was dissolved in Tris-HCl buffer, and stirred at room temperature for 10 h to achieve pre-polymerization of epicatechin in the air environment, and a solution of epicatechin with a mass fraction of 1% was obtained. Add 200 g of tricalcium phosphate nanoparticles into the epicatechin solution with a mass fraction of 1% and mix evenly according to the mass ...

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Abstract

The invention belongs to the field of biomaterial preparation and biomedical application, and particularly relates to a fibroin bone cement biological adhesive with excellent wet state adhesion and immediate high strength mechanical properties and a preparation method thereof. According to the preparation method, polyphenols / hydroxyapatite or polyphenols / calcium phosphate is introduced into a fibroin solution system to prepare the fibroin bone cement biological adhesive with super strong adhesion. The adhesive has excellent wet state adhesion, and the novel injectable bone cement adhesive is provided for bone and joint hard tissue injury caused by trauma and pathological diseases. The adhesive can also be used as a hemostatic adhesive material for tissues such as skins, organs, blood vessels, nerves, muscles and mucous membranes.

Description

technical field [0001] The invention belongs to the fields of biological material preparation and biomedical application, and in particular relates to synthesizing a silk fibroin bone cement bioadhesive with excellent wet-state bonding and immediate high-strength mechanical properties and a preparation method thereof. Background technique [0002] Hard tissue injuries such as bones and joints and soft tissue injuries caused by trauma and pathological diseases have become public health problems worldwide. It is an ideal means to cure bone injury diseases by filling the bone injury site with bone repair materials and rebuilding the integrity and stability of the bone structure. Autologous bone grafting and allogeneic bone grafting are considered to be the "gold standard" treatment options for bone defect repair, but there are disadvantages such as insufficient donors, immune rejection, and inability to provide immediate stability. At present, the clinical treatment methods fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/12A61L27/22A61L27/18
CPCA61L27/12A61L27/18A61L27/227A61L27/50A61L2400/04A61L2430/02A61L2430/24C08L89/00A61L24/0084
Inventor 杨黄浩白树猛黄晓卫
Owner FUZHOU UNIV
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