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Hyaluronic acid supramolecular hydrogel for three-dimensional culture of chondrocytes, and preparation and application of hyaluronic acid supramolecular hydrogel

A technology of supramolecular hydrogel and hyaluronic acid, which is applied in the direction of cell culture support/coating, bone/connective tissue cells, animal cells, etc., can solve the problem of limiting chondrocyte proliferation, cartilage matrix secretion, and poor mechanical biocompatibility and other problems, to improve mechanical compatibility, prevent cell leakage, and achieve good results

Active Publication Date: 2020-11-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional chemically cross-linked hydrogels need to add cross-linking agents or initiators to form a static cross-linked network, which is often brittle and has poor mechanical and biocompatibility in the dynamic mechanical environment in vivo. Most importantly, studies have shown that Formation of a static elastic network limits chondrocyte proliferation and secretion of cartilage matrix

Method used

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  • Hyaluronic acid supramolecular hydrogel for three-dimensional culture of chondrocytes, and preparation and application of hyaluronic acid supramolecular hydrogel
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  • Hyaluronic acid supramolecular hydrogel for three-dimensional culture of chondrocytes, and preparation and application of hyaluronic acid supramolecular hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A method for preparing a hyaluronic acid supramolecular gel for three-dimensional culture of chondrocytes, comprising the steps of:

[0067] (1) Add 0.1g of methacrylic anhydride-modified hyaluronic acid (HAMA) into 180mL of deionized water, and stir at room temperature to dissolve completely;

[0068] (2) Add 0.1 g of double bond functionalized UPy macromonomer (UPyMA) to 5 mL of deionized water, add 200 μL of 5M sodium hydroxide solution, stir to dissolve completely, and add to step (1);

[0069] (3) 0.3 g of 2-(2-methoxyethoxy) ethyl methacrylate (DEGMA, purchased from Aladdin Co., Ltd.) was dissolved in 5 mL of deionized water, and added to step (2);

[0070] (4) Dissolve 20 mg of potassium persulfate (KPS) in 5 mL of deionized water and add to step (3);

[0071] (5) Nitrogen deoxygenation in the reaction solution of step (4) was carried out for 40min;

[0072] (6) Dissolve 100 μL of tetramethylethylenediamine (TEMED) in 5 mL of deionized water, and add to step (5...

Embodiment 2

[0078] A method for preparing a hyaluronic acid supramolecular gel for three-dimensional culture of chondrocytes, comprising the steps of:

[0079] (1) Add 0.4 g of methacrylic anhydride-modified hyaluronic acid (HAMA) into 180 mL of deionized water, and stir at room temperature to dissolve completely;

[0080] (2) Add 0.1 g of double bond functionalized UPy macromonomer (UPyMA) to 5 mL of deionized water, add 200 μL of 5M sodium hydroxide solution, stir to dissolve completely, and add to step (1);

[0081] (3) 0.6 g of 2-(2-methoxyethoxy) ethyl methacrylate (DEGMA, purchased from Aladdin Co., Ltd.) was dissolved in 5 mL of deionized water, and added to step (2);

[0082] (4) Dissolve 40 mg of potassium persulfate (KPS) in 5 mL of deionized water and add to step (3);

[0083] (5) Nitrogen deoxygenation in the reaction solution of step (4) was carried out for 40min;

[0084] (6) Dissolve 200 μL of tetramethylethylenediamine (TEMED) in 5 mL of deionized water, and add to step ...

Embodiment 3

[0090] A method for preparing a hyaluronic acid supramolecular gel for three-dimensional culture of chondrocytes, comprising the steps of:

[0091] (1) Add 0.2 g of methacrylic anhydride-modified hyaluronic acid (HAMA) into 180 mL of deionized water, and stir at room temperature to dissolve completely;

[0092] (2) Add 0.1 g of double bond functionalized UPy macromonomer (UPyMA) to 5 mL of deionized water, add 200 μL of 5M sodium hydroxide, stir to dissolve completely, and add to step (1);

[0093] (3) 0.5 g of 2-(2-methoxyethoxy) ethyl methacrylate (DEGMA, purchased from Aladdin Co., Ltd.) was dissolved in 5 mL of deionized water and added to step (2);

[0094] (4) Dissolve 40 mg of potassium persulfate (KPS) in 5 mL of deionized water and add to step (3);

[0095] (5) Nitrogen deoxygenation in the reaction solution of step (4) was carried out for 40min;

[0096] (6) Dissolve 200 μL of tetramethylethylenediamine (TEMED) in 5 mL of deionized water, and add to step (5) after ...

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Abstract

The invention belongs to the technical field of regenerative medical materials, and discloses hyaluronic acid supramolecular hydrogel for three-dimensional culture of chondrocytes, and preparation andapplication of the hyaluronic acid supramolecular hydrogel. The method comprises the following steps: with water as a reaction medium, carrying out a copolymerization reaction on methacrylic anhydride modified hyaluronic acid, a double-bond functionalized ureido pyrimidinone functional monomer and 2-(2-methoxyethoxy)ethyl methacrylate under the action of an initiating system, and carrying out subsequent treatment to obtain the hyaluronic acid supramolecular hydrogel. The hydrogel is used in the field of cartilage tissue engineering, and is used for three-dimensional culture of chondrocytes and preparation of cartilage repair materials. The hydrogel disclosed by the invention has sol-gel conversion and self-repairing properties, uniform dispersion of the chondrocytes can be easily realizedby sol at a low temperature, and the sol can undergo phase change to form gel after being conveyed to a cartilage injury part, so in-situ fixation of the chondrocytes is realized; and the formation of a dynamic three-dimensional microenvironment of the hydrogel is beneficial for the maintenance of chondrocyte functions and the secretion of cartilage characteristic matrixes.

Description

technical field [0001] The invention relates to the technical field of regenerative medical materials, in particular to a hyaluronic acid supramolecular hydrogel for three-dimensional culture of chondrocytes, a preparation method and application thereof. Background technique [0002] Articular cartilage damage caused by acute trauma, strain or disease is a common joint disease in orthopedics. If the articular cartilage injury is not treated in time, the degradation of the cartilage matrix will easily lead to osteoarthritis, and eventually only joint replacement can be accepted, and severe cases may even lead to permanent disability. Different from the huge regenerative potential of bone tissue, articular cartilage has very weak regeneration ability after cartilage injury due to the lack of blood vessel, nerve and lymphatic network structure. Therefore, in the field of regenerative medicine, cartilage defect repair has always been a research hotspot. Autologous chondrocyte ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08F251/00C08F220/36C08F220/28C12N5/077C08L51/02
CPCC08J3/075C08F251/00C12N5/0655C12N2513/00C12N2533/80C08J2351/02C08F220/36C08F220/282
Inventor 任力滕丽晶陈云华
Owner SOUTH CHINA UNIV OF TECH
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