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Piezoelectric composite dressing for promoting peripheral nerve repair and wound healing and loading exosome of traditional Chinese medicine and preparation method

A technology for wound healing and peripheral nerves, applied in the fields of medical formula, medical science, drug delivery, etc., can solve problems such as difficult to achieve therapeutic effect, blank, etc., achieve broad medical and commercial prospects, promote proliferation, and promote wound healing.

Active Publication Date: 2019-11-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is often difficult to achieve a good therapeutic effect simply by virtue of the properties of the material itself.
In clinical use, synergy with drugs is also required, but research in this field is still blank

Method used

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  • Piezoelectric composite dressing for promoting peripheral nerve repair and wound healing and loading exosome of traditional Chinese medicine and preparation method
  • Piezoelectric composite dressing for promoting peripheral nerve repair and wound healing and loading exosome of traditional Chinese medicine and preparation method
  • Piezoelectric composite dressing for promoting peripheral nerve repair and wound healing and loading exosome of traditional Chinese medicine and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: A piezoelectric-conductive gel that promotes peripheral nerve repair and a composite dressing loaded with traditional Chinese medicine exosomes and a preparation method thereof, comprising the following steps:

[0050] 1) react gelatin with methacrylic anhydride, then dialyze in a 8k-14kDa dialysis bag at room temperature for 1 week, and then freeze-dry to prepare methacrylated gelatin;

[0051] 2) First wash the privet, squeeze the juice, and pass through a 100-mesh sieve; then through the differential centrifugation method, centrifuge at 1000g and 12000g successively, take the supernatant and centrifuge at 100000g with an ultracentrifuge, and then use the Resuspended in PBS. Prepare a density gradient solution with sucrose, and then ultracentrifuge at 200,000g for 60 minutes; then take exosomes of different components and centrifuge at 50,000g for 60 minutes to prepare traditional Chinese medicine exosomes;

[0052] 3) by HAuCl 4 With CTAB, NaBH 4 Pre...

Embodiment 2

[0058] Embodiment 2: A piezoelectric-conductive gel that promotes peripheral nerve repair and a composite dressing loaded with traditional Chinese medicine exosomes and a preparation method thereof, comprising the following steps:

[0059] 1) reacting gelatin with methacrylic anhydride, then dialysis with 8k-14kDa dialysis bag at room temperature for 2 weeks, and then freeze-drying to prepare methacrylated gelatin;

[0060] 2) Wash the salvia miltiorrhiza, squeeze the juice, and pass through a 200-mesh sieve; then through the differential centrifugation method, centrifuge at 2000g and 10000g successively, take the supernatant and centrifuge at 120000g with an ultracentrifuge, and then wash the precipitate with PBS Resuspended. Use CsCl gradient solution, and then ultracentrifuge at 100,000g for 60 minutes; then take exosomes of different components and centrifuge at 100,000g for 60 minutes to prepare traditional Chinese medicine exosomes;

[0061] 3) by HAuCl 4 With CTAB, Na...

Embodiment 3

[0069] Embodiment 3: A piezoelectric-conductive gel that promotes peripheral nerve repair and a composite dressing loaded with traditional Chinese medicine exosomes and a preparation method thereof, comprising the following steps:

[0070] 1) react gelatin with methacrylic anhydride, then dialyze in a dialysis bag of 8k-14kDa for 1 week at room temperature, and then freeze-dry to prepare methacrylated gelatin;

[0071] 2) First wash the rehmannia glutinosa, squeeze the juice, and pass through a 150-mesh sieve; then through the differential centrifugation method, centrifuge at 2000g and 10000g successively, take the supernatant and centrifuge at 150000g in an ultracentrifuge, and then weigh the precipitate with PBS hanging. Use a sucrose gradient solution, and then ultracentrifuge at 150,000g for 120 minutes; then take exosomes of different components and centrifuge at 150,000g for 60 minutes to prepare traditional Chinese medicine exosomes;

[0072] 3) by HAuCl 4 With CTAB, ...

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Abstract

The invention provides a piezoelectric composite dressing for promoting peripheral nerve repair and wound healing and loading exosome of traditional Chinese medicine and a preparation method. The preparation method comprises the following steps that gelatin and methacrylic anhydride react to prepare methacrylate gelatin; the exosome of the Chinese medicine is prepared through differential centrifugation;a conductive pregel system is prepared through the recombination of gold nanorods, the exosome and the methacrylate gelatin; and the conductive pregel and a polyvinylidene fluoride ethylene piezoelectric thin film arerecombined, and the piezoelectric composite dressing is prepared. The dressing prepared by the preparation method is a novel dressing for piezoelectric stimulation composite medicine action, polyvinylidene fluoride ethylene produces electricity potential to stimulate a wound area through the conductive gel, wound healing and nerve regeneration are promoted with the exosomeof the traditional Chinese medicine, and the problems in the current wound repair and nerve repair process can be effectively solved. Therefore, the composite dressing has a wide application value inthe treatment of trauma and nerve repair.

Description

technical field [0001] The invention relates to a piezoelectric composite dressing that promotes peripheral nerve repair and wound healing and loads traditional Chinese medicine exosomes and a preparation method. Background technique [0002] The skin is the first line of defense of the human body and is also the largest organ of the human body. It has multiple functions such as sensation and protection. However, in daily life, the skin is often affected by various external factors, which often cause trauma and bring heavy pressure and burden to individuals and society. However, wound healing is a complex and dynamic process, including a variety of tissues and cells involved in the regulation. However, the uncontrolled adjustment of the wound repair process leads to serious scars after wound healing, and the inability to regenerate appendages, resulting in repair obstacles. At the same time, a complex neural network is also distributed on the surface of the skin, mainly in...

Claims

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Application Information

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IPC IPC(8): A61L15/40A61L15/32A61L15/18A61L15/24A61L15/42A61L15/44
CPCA61L15/18A61L15/24A61L15/325A61L15/40A61L15/42A61L15/44A61L2300/30A61L2300/412A61L2300/602A61L2400/12C08L27/16
Inventor 彭丽华许学寒
Owner ZHEJIANG UNIV
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