Preparation method of composite gel micro-spheres as well as composite gel micro-spheres and application thereof

A technology of composite gel and microspheres, which is applied in the directions of inactive medical preparations, pharmaceutical formulations, inactive high molecular compounds, etc. glue and other problems, to achieve the effect of low cytotoxicity, increased strength, and short time

Pending Publication Date: 2019-08-02
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the method for preparing silk fibroin hydrogel in the prior art cannot produce silk fibroin hydrogel with good biocompatibility and mechanical properties

Method used

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  • Preparation method of composite gel micro-spheres as well as composite gel micro-spheres and application thereof
  • Preparation method of composite gel micro-spheres as well as composite gel micro-spheres and application thereof
  • Preparation method of composite gel micro-spheres as well as composite gel micro-spheres and application thereof

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Embodiment 1

[0050] This embodiment provides a method for preparing composite gel microspheres, wherein the composite gel microspheres are composite gel microspheres of silk fibroin and collagen, and the preparation method specifically includes the following steps:

[0051] S1, modifying the silk fibroin to obtain a methacrylic anhydride-treated silk fibroin solution

[0052] (1) Add 5 g of degummed silk to a 9.3 M lithium bromide solution for dissolution, heat treatment at 60° C. for 1 hour, and prepare a silk fibroin solution;

[0053] (2) Add 1.5ml of glycidyl methacrylate to the silk fibroin solution, stir at a speed of 3000rpm to mix the solution evenly, and then heat treatment at 60°C for 4h to obtain methacrylic acid anhydride silk fibroin solution.

[0054] S2, adding methacrylic anhydrided collagen solution and photoinitiator to the methacrylic anhydrided silk fibroin solution to obtain a prepolymer solution

[0055] (3) Place the methacrylic anhydrided silk fibroin solution in ...

Embodiment 2

[0062] This embodiment provides a method for preparing composite gel microspheres, wherein the composite gel microspheres are composite gel microspheres of silk fibroin and hyaluronic acid, and the preparation method specifically includes the following steps:

[0063] S1, modifying the silk fibroin to obtain a methacrylic anhydride-treated silk fibroin solution

[0064] (1) Add 5 g of degummed silk to a 9.3 M lithium bromide solution for dissolution, heat treatment at 60° C. for 1 hour, and prepare a silk fibroin solution;

[0065] (2) Add 2.25ml glycidyl methacrylate to the silk fibroin solution, stir at a speed of 3000rpm to mix the solution evenly, and then heat treatment at 60°C for 4h to obtain methacrylic acid anhydride silk fibroin solution.

[0066] S2, adding hyaluronic acid solution and photoinitiator of methacrylic anhydride to the silk fibroin solution of methacrylic anhydride to obtain prepolymer solution

[0067] (3) Place the methacrylic anhydrided silk fibroi...

Embodiment 3

[0074] This embodiment provides a method for preparing composite gel microspheres, wherein the composite gel microspheres are composite gel microspheres of silk fibroin and gelatin, and the preparation method specifically includes the following steps:

[0075] S1, modifying the silk fibroin to obtain a methacrylic anhydride-treated silk fibroin solution

[0076] (1) Add 5 g of degummed silk to a 9.3 M lithium bromide solution for dissolution, heat treatment at 60° C. for 1 hour, and prepare a silk fibroin solution;

[0077] (2) Add 1.5ml of glycidyl methacrylate to the silk fibroin solution, stir at a speed of 3000rpm to mix the solution evenly, and then heat treatment at 60°C for 4h to obtain methacrylic acid anhydride silk fibroin solution.

[0078] S2, adding methacrylic anhydrided gelatin solution and photoinitiator to the methacrylic anhydrided silk fibroin solution to obtain a prepolymer solution

[0079] (3) Place the methacrylic anhydrided silk fibroin solution in a ...

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Abstract

The invention discloses a preparation method of composite gel micro-spheres. The preparation method comprises the following steps: performing modification treatment on silk fibroins to obtain a methacrylate anhydride silk fibroin solution; adding a methacrylate anhydride bio-polymerized molecular solution and a photo-initiator to obtain a pre-polymer solution; preparing composite micro-droplets bythe pre-polymer solution, performing radiation treatment with blue light to obtain the silk fibroin protein and the bio-polymerized molecule composite gel microspheres. The composite gel microsphereswith high mechanical property and good biocompatibility can be prepared by the method. The invention further discloses a cell coating method based on the preparation method; composite gel microsphere-coated cells are small in damage and low in toxicity and can be obtained with good survival and proliferation. The invention also discloses the composite gel micro-spheres prepared by the method andapplication thereof to cell 3D culture, 3D printing and use as biomedical materials; the composite gel micro-spheres have a wide application to the biomedical field through good biocompatibility and mechanical property.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a preparation method of composite gel microspheres, a cell coating method based on the preparation method of composite gel microspheres, composite gel microspheres and applications of composite gel microspheres. Background technique [0002] Silk fibroin is a fibrous protein obtained by degumming silkworm silk. It is mainly composed of 18 kinds of amino acids arranged in a certain order, of which 85% of the amino acids are alanine (Ala), serine (Ser) and Glycine (Gly), etc., and form a certain crystalline region with a regular structure; the amorphous region is composed of a large number of tyrosine (Tyr) and tryptophan with large side groups that exist in an amorphous structure. (Try) and phenylalanine (Phe). Silk fibroin has high protein purity, less biological impurities, good biocompatibility and biodegradability, and has a wide range of applications from clothing raw m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/12C08L89/00C08L5/08A61L26/00A61L27/20A61L27/22A61L27/24A61L27/52A61K47/36A61K47/42
CPCA61K47/36A61K47/42A61L26/0023A61L26/0033A61L26/0038A61L26/0047A61L26/008A61L27/20A61L27/222A61L27/227A61L27/24A61L27/52C08J3/12C08J2389/00C08J2405/08C08J2489/00C08L89/00C08L5/08
Inventor 张涛黎海文蒋克明周武平刘聪张志强
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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