Nutrient black soya bean powder prepared by fermentation of bacillus natto and preparation method thereof
A technology of natto bacteria and black bean flour, which is applied in food preparation, application, food science and other directions, can solve the problems of low nutritional value, low degree of peptideization, and unfavorable circulation and preservation of commodities, and achieves rich nutrition, assists in dissolving thrombosis, and protects Effects of liver damage
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[0022] The materials used are: black soybean, papain (2000U / g), cellulase (2000U / g), lysozyme (5000U / g), Bacillus natto (CICC No.10459).
[0023] Weigh 500 grams of black soybeans, peel the black soybeans, and the peeling rate must be above 70%; add 5 times of deionized water (2500ml) to the peeled soybeans, and wet ultrafine grinding, so that the median diameter of the particles is below 20um; the crushed soybeans The material was sterilized at a temperature of 110-121°C for 30 minutes, and after cooling, it was inserted into Bacillus natto (Bacillus natto (CICC No. 10459) was expanded and cultivated in potato liquid medium for 24 hours, and the expanded Bacillus natto was inoculated). Fermented for 3 days at a temperature of 30°C and a shaker speed of 160rpm. After the fermentation, the nattokinase activity in the fermentation broth was detected by the fibrin plate method to be 10IU / ml.
[0024] Add 2500ml of deionized water to the fermentation broth to make the ratio of mat...
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