Functional lactobacillus plantarum and preparation method of compound bacterial powder of functional lactobacillus plantarum
A technology of Lactobacillus plantarum and compound bacteria powder, which is applied in the directions of drug combination, microorganism-based method, biochemical equipment and method, etc., can solve the problems such as compounding of natto and red yeast rice that are not involved
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Embodiment 1
[0034] Embodiment 1, the preparation method of Lactobacillus plantarum subsp. plant bacterial powder
[0035] 1. Preparation of Lactobacillus plantarum subsp. planta bacterial powder
[0036] (1) Strain activation: Inoculate the Lactobacillus plantarum subsp. Zhang-LL strain after activated and expanded culture into 2L modified MRS medium, and use a 5L fully automatic fermenter for high-density fermentation, with the temperature controlled at 34°C , the pH of the fermented liquid is 6.5, the stirring speed is 150r / min, and fermented for 20-28 hours to obtain the fermented liquid.
[0037]Improved MRS liquid medium formula: tryptone 10.0g, beef extract 10.0g, yeast extract powder 5.0g, triammonium citrate 2.0g, glucose 20.0g, Tween 801.0mL, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g , magnesium sulfate 0.5g, manganese sulfate 0.25g, water 1L, pH6.5, 0.1Mpa sterilization for 15min.
[0038] (2) Collecting the sludge: put the fermentation broth in a centrifuge, ce...
Embodiment 2
[0042] The preparation method of embodiment 2, bacillus natto natto powder
[0043] 1. Preparation of Bacillus natto natto powder
[0044] (1) Strain activation and expansion culture: put the slant strain of Bacillus natto into a 100mL Erlenmeyer flask containing 20mL of seed medium, shake the flask at 37°C and 200r / min for 24-36h; then take 1mL of seed The solution was transferred into a 250mL Erlenmeyer flask equipped with 50mL of fermentation medium, and cultured on a shaker at 37°C and 200r / min for 24-36h to obtain the expanded seed solution of Bacillus natto.
[0045] In the above method, the seed medium formula: 2g of peptone, 3g of beef extract, 3g of glucose, 5g of NaCl, 1L of water, pH 7.0, sterilized at 0.1Mpa for 15min.
[0046] (2) Fermentation: crush edible soybeans to 100 mesh, add water in an appropriate proportion, sterilize at 0.1Mpa for 60 minutes, cool to 40°C, inoculate Bacillus natto with expanded seed liquid, stir evenly, and ferment at 37°C for 24 hours...
Embodiment 3
[0051] Embodiment 3, the preparation method of Monascus purpura Monascus powder
[0052] 1. Preparation of Monascus purpura Monascus powder
[0053] (1) Strain activation and expansion culture: Inoculate the slant strain of Monascus purpura onto malt agar medium, culture at 30°C for 48 hours, and store at 4°C; pick the activated strain and inoculate it into the solid spore medium, After culturing at 30° C. for 5 days, the spores were washed with physiological saline to obtain Monascus purpura spore liquid.
[0054] (2) Solid-state fermentation: centrifuge the spore liquid at 10 000 r / min for 20 minutes, take the spore sludge, add it to the solid-state fermentation medium, stir evenly, and culture at 32°C for 12-15 days to obtain red yeast rice.
[0055] In the above method, the solid-state fermentation medium formula is: indica rice 70% + buckwheat 30% as the base medium, and 0.1% glucose, 0.3% 501 rapeseed peptone and 0.04% ZnSO 4 .
[0056] (3) Drying and crushing: after ...
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