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Method for preparing high-activity nattokinase by taking black beans as raw material

A technology of nattokinase and high activity is applied in the field of preparing high-activity nattokinase, which can solve the problems of low enzyme activity and large ammonia odor, and achieve the effects of short fermentation period, low ammonia odor and rich nutrition of products.

Active Publication Date: 2013-07-17
湖北真福医药有限公司
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AI Technical Summary

Problems solved by technology

At present, the production of nattokinase by solid fermentation method mostly uses soybean as raw material for fermentation, which has the disadvantages of low enzyme activity and strong ammonia odor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Bacillus subtilis Cultivation of QK02 Seed Solution

[0033] The seed solution is prepared by culturing the slant strain in shake flasks. The culture conditions were as follows: LB medium was used as the seed medium, the pH was 7.2, the liquid volume in the Erlenmeyer flask was 25mL / 250mL, the culture temperature was 37°C, the shaker speed was 180r / min, and the culture period was 16h.

[0034] (2) Solid fermentation

[0035] Take 10kg of full-grained black beans for impurity removal and cleaning, add 30L of the above-mentioned nutrient solution to soak for 16 hours; drain the soaked black beans and put them in a cooking equipment for 40 minutes at 0.1MPa and 121°C; cool naturally to 50°C Inoculate 10% of the weight of black beans under sterile conditions Bacillus subtilis The QK02 seed liquid is then fermented in the fermentation equipment, the thickness of the material is controlled to be 3cm, the temperature is 37°C, the humidity is 75%, and the fermentation ...

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Abstract

The invention discloses a method for preparing high-activity nattokinase by taking black beans as raw material, belonging to the technical field of biology. The method comprises the following steps: soaking the black beans by using a special nutrient solution, cooking the black beans after soaking and further inoculating bacillus natto to perform fermentation, wherein the special nutrient solution comprises the following components: lactose, tryptone, Na2HPO4, NaH2PO4, MgSO4 and CaCl2. The method further comprises the steps of performing extraction separation and freeze-drying on a fermentation product. The nattokinase obtained by adopting the method has high enzyme activity, which can achieve 42500IU / g, and the nattokinase has the advantages of high enzyme activity, low ammonia smell and good taste in comparison with the nattokinase produced by taking yellow beans as the raw material and performing a traditional fermentation method; and the content of nutritional components in obtained fermented black bean residue is also obviously improved. The method has the advantages of short fermentation period, simple preparation process and high bioavailability, and is conductive to industrial production.

Description

[0001] technical field [0002] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing highly active nattokinase from black soybeans. Background technique [0003] Both black beans and soybeans belong to soybeans, but black beans have advantages in both nutritional quantity and quality. Black soybeans are rich in isoflavones and flavonoids, a large amount of dietary fiber, unsaturated fatty acids, rich in protein, various minerals and trace elements, especially the black seed coat contains a variety of pigments and saponins and unique active substances. In addition, black beans contain a large amount of soybean globulin, linoleic acid, and linolenic acid that can reduce neutral fat. These useful ingredients have the effects of softening blood vessels, dilating blood vessels, and promoting blood circulation. Vascular disease, anti-oxidation, anti-aging, increase gastrointestinal motility and other effects. Black soybeans are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/56C12R1/125
Inventor 王业富高丽
Owner 湖北真福医药有限公司
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